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miRNA-218 Inhibits Osteosarcoma Cell Migration and Invasion by Down-regulating of TIAM1, MMP2 and MMP9

  • Jin, Jie (Department of Orthopedics, Zhongnan Hospital of Wuhan University) ;
  • Cai, Lin (Department of Orthopedics, Zhongnan Hospital of Wuhan University) ;
  • Liu, Zhi-Ming (Department of Orthopedics, Tongren Hospital of Wuhan) ;
  • Zhou, Xue-Song (Department of Orthopedics, Tongren Hospital of Wuhan)
  • Published : 2013.06.30

Abstract

Deregulated miRNAs participate in osteosarcoma genesis. In this study, the expression of miRNA-218 in human osteosarcomas, adjacent normal tissues and Saos-2 human osteosarcoma cells was first assessed. Then the precise role of miRNA-218 in osteosarcoma cells was investigated. Upon transfection with a miR-218 expression vector, the proliferation of Saos-2 human osteosarcoma cells determined using the ATPlite assay was significantly suppressed, whilw migration of Saos-2 cells detected by wound healing and invasion determined using transwells were dramatically inhibited. Potential target genes of miR-218 were predicted and T-cell lymphoma invasion and metastasis 1 (TIAM1) and matrix metalloproteinase 2 (MMP2) and 9 (MMP9) were identified. This was confirmed by western blotting, which showed that miR-218 expression inhibited TIAM1, MMP2 and MMP9 protein expression. Collectively, these data suggest that miR-218 acts as a tumor suppressor in osteosarcomas by down-regulating TIAM1, MMP2 and MMP9 expression.

Keywords

miRNA-218;osteosarcoma;TIAM1;proliferation;migration;invasion;MMP

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