Expression and Significance of Hypoxia Inducible Factor-1α and Lysyl Oxidase in Non-small Cell Lung Cancer

  • Ping, Wei (Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Jiang, Wen-Yang (Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Chen, Wen-Shu (Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Sun, Wei (Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology) ;
  • Fu, Xiang-Ning (Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology)
  • Published : 2013.06.30


Object: To detect expression of hypoxia inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$) and lysyl oxidase (LOX) in non-small cell lung cancer (NSCLC) and explore their roles in prognosis. Methods: The mRNA levels of HIF-$1{\alpha}$ and LOX were investigated by real-time reverse-transcriptase polymerase chain reaction in 40 cases of tumour and paired normal tissues. In addition, protein expression of HIF-$1{\alpha}$ and LOX was examined by immunohistochemistry in 82 cases of tumour and 45 paired normal tissues. The relationship between HIF-$1{\alpha}$ or LOX and clinicopathologic characteristics, as well as the correlation between HIF-$1{\alpha}$ and LOX, were also examined. Kaplan-Meier survival curves and the log-rank test were used to analyze progression-free survival. Results: HIF-$1{\alpha}$ or LOX mRNA levels in tumor tissues was significantly higher than those in paired normal tissues (p<0.01). Positive HIF-$1{\alpha}$ or LOX protein expression in tumor tissues was noted in 46/82 (56.1%) and 49/82 (59.8%) of the cases, respectively, being significantly higher than those in paired normal tissues (p<0.05). There was significant correlation between the expression of HIF-$1{\alpha}$ or LOX and tumor size, lymph node metastasis and pathological stage (p<0.05). The expression of HIF-$1{\alpha}$ and LOX had a significant inverse impact on survival of patients with NSCLC. Conclusion: HIF-$1{\alpha}$ and LOX may play a pivotal role in the development of NSCLC, and may act in synergy to promote the progression of NSCLC.


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