Anti-inflammatory and Anticancer Activities of Ethanol Extract of Pendulous Monkshood Root in vitro

  • Huang, Xian-Ju (College of Pharmacy, South-Central University for Nationalities) ;
  • Ren, Wei (College of Pharmacy, South-Central University for Nationalities) ;
  • Li, Jun (College of Pharmacy, South-Central University for Nationalities) ;
  • Chen, Lv-Yi (College of Pharmacy, South-Central University for Nationalities) ;
  • Mei, Zhi-Nan (College of Pharmacy, South-Central University for Nationalities)
  • Published : 2013.06.30


Aim: Pendulous monkshood root is traditionally used for the treatment of several inflammatory pathologies such as rheumatisms, wounds, pain and tumors in China. In this study, the anti-inflammatory and anticancer activities and the mechanism of crude ethanol extract of pendulous monkshood root (EPMR) were evaluated and investigated in vitro. Materials and Methods: The cytotoxic effects of EPMR on different tumor cell lines were determined by the MTT method. Cell apoptosis and cell nucleus morphology were assessed by Hoechst 33258 staining. Moreover, nitric oxide (NO) levels and intracellular oxidative stress in peritoneal macrophages were determined to further elucidate mechanisms of action. Results: The data showed that EPMR could produce significant dose-dependent toxicity on three kinds of tumor cells. Furthermore, EPMR displayed obvious anti-inflammatory effects on LPS-induced mouse peritoneal macrophages at the dosage of 4 - 200 ${\mu}g/mL$. The results demonstrated the therapeutic potential of Pendulous Monkshood Root on cancer and inflammatory diseases. Conclusion: Our results indicate that EPMR has anti-inflammatory and anticancer properties, suggesting that pendulous monkshood root may be a useful anti-tumor and anti-inflammatory reagent in the clinic.


Pendulous monkshood root;anti-inflammatory;anticancer;mouse peritoneal macrophages


Supported by : National Natural Science Foundation of China


  1. AMERI A (1998). The effects of aconitum alkaloids on the central nervous system. Prog Neurobiol, 56, 211-35.
  2. Araki T, Yamamoto A, Yamada M (1987). Accurate determination of DNA content in single cell nuclei stained with Hoechst 33258 fluorochrome at high salt concentration. Histochem J, 87, 331-8.
  3. Balkwill FR, Mantovani A (2012). Cancer-related inflammation: Common themes and therapeutic opportunities. Cancer Immunotherapy, 22, 33-40.
  4. Duckworth CA, Clyde D, Pritchard DM (2012). CD24 is expressed in gastric parietal cells and regulates apoptosis and the response to Helicobacter felis infection in the murine stomach. Am J Physiol Gastrointest Liver Physiol, 303, 915-26.
  5. Grivennikov SI, Greten FR, Karin M (2010). Immunity, inflammation, and cancer. Cell, 140, 883-99.
  6. Grote VA, Kaaks R, Nieters A, et al (2012). In flammation marker and risk of pancreatic cancer: a nested case control study within the EPIC cohort. Br J Cancer, 106, 1866-74.
  7. Hikino H, Ito T, Yamada C, et al (1979). Analgesic principles of Aconitum roots. J Pharmaco Dyn, 2, 78-83.
  8. Huang X, Li Q, Zhang Y, et al (2008). Neuroprotective effects of cactus polysaccharide on oxygen and glucose deprivation induced damage in rat brain slices. Cell Mol Neurobiol, 28, 559-68.
  9. Hussain SP, Trivers GE, Hofseth LJ, et al (2004). Nitric oxide, a mediator of inflamma tion, suppresses tumorigenesis. Cancer Research, 64, 6849-53.
  10. Kim YH, Woo KJ, Lim JH, et al (2005). 8-Hydroxyquinoline inhibits iNOS expression and nitric oxide production by down-regulating LPS-induced activity of NF-kapp aB and C/EBPbeta in Raw 264.7 cells. Biochem Biophys Res Commun, 329, 591-7.
  11. Kipanyula MJ, Etet PFS, Vecchio L, et al (2012). Signaling pathways bridging microbial-triggered inflammation and cancer. Cell Signal, 25, 403-6.
  12. Kundu JK, Surh YJ (2012). Emerging avenues linking inflammation and cancer. Free Radic Biol Med, 52, 2013-37.
  13. Liu RH, Hotchkiss JH (1995). Potential genotoxicity of chronically elevated nitric oxide: a review. Mutat Res, 339, 73-89.
  14. Mantovani A, Allavena P, Sica A, et al (2008). Cancer-related inflammation. Nature, 454, 436-44.
  15. Meiners S, Marone M, Rittenhouse JL, et al (1993). Regulation of astrocytic tenascin by basic fibroblast growth factor. Dev Biol, 160, 480-93.
  16. Meiners S, Powell EM, Geller HM (1995). A distinct set of tenascin/CS-6-PG-rich astrocytes restricts neuronal growth in vitro. J Neurosci, 15, 8096-108.
  17. Narayan C, Kumar A (2012). Constitutive over expression of IL-1beta, IL-6, NF-kappa B, and Stat3 is a potential cause of lung tumor genesis in urethane (ethyl carbamate) induced Balb/c mice. J Carcinog, 11, 9.
  18. Ramana KV, Reddy ABM, Tammali R, et al (2007). Aldose reductase mediates endotoxin-induced production of nitric oxide and cytotoxicity in murine macrophages. Free Radic Biol Med, 42, 1290-302.
  19. Sato H, Ohizumi Y, Hikino H (1979). Mechanisms of mesaconitine-induced contractile response in guinea pig. vas deferens. Eur J Pharmac, 55, 83-92.
  20. Singhuber J, Zhu M, Prinz S, et al (2009). Aconitum in traditional Chinese medicine: a valuable drug or an unpredictable risk? J Ethnopharmacol, 126, 18-30.
  21. Sodir NM, Swigart LB, Karnezis AN, et al (2011). Endogenous Myc maintains the tumor microenvironment. Genes Dev, 25, 907-16.
  22. Tamir S, Tannenbaum SR (1996). The role of nitric oxide (NO-) in the carcinogenic process. Biochim Biophys Acta, 1288, F31-6.
  23. Xiao P, Wang F, Gao F, et al (2006). A pharmacophylogenteic study of Aconitum L (Ranunculaceae) from China. Acta Phytotaxonomica Sinica, 44, 1-46.
  24. Yao G, Yang L, Hu Y, et al (2006). Nonylphenol-induced thymocyte apoptosis involved caspase-3 activation and mitochondrial depolarization. Mol Immunol, 43, 915-26.

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