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KAI1/CD82 and MRP1/CD9 Serve as Markers of Infiltration, Metastasis, and Prognosis in Laryngeal Squamous Cell Carcinomas

  • Zhang, Bing-Hui (Department of Otolaryngology/Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University) ;
  • Liu, Wei (Experiment Center, the Second Affiliated Hospital of Harbin Medical University) ;
  • Li, Liang (Department of Otolaryngology/Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University) ;
  • Lu, Jian-Guang (Department of Otolaryngology/Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University) ;
  • Sun, Ya-Nan (Department of Otolaryngology/Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University) ;
  • Jin, De-Jun (Department of Otolaryngology/Head and Neck Surgery, the Second Affiliated Hospital of Harbin Medical University) ;
  • Xu, Xiu-Yu (Head and Neck Surgery, the Third Affiliated Hospital of Harbin Medical University)
  • Published : 2013.06.30

Abstract

Objective: The current study explored the expression of KAI1/CD82 and MRP1/CD9 and its significance in laryngeal squamous cell carcinoma (LSCC). Methods: The expression levels of KAI1/CD82 and MRP1/CD9 in 100 LSCC tissue specimens, as well as in 30 para-LSCC non-carcinomatous tissue specimens randomly taken from the patients, were assessed using the quantitative polymerase chain reaction (Q-PCR) and immunohistochemistry and correlations with pathological parameters of LSCC and their influence on survival function were analyzed. Results: KAI1/CD82 and MRP1/CD9 showed basically consistent changes in both mRNA and protein expression. Their expression in the 30 LSCC specimens was significantly lower compared with that in the corresponding non-carcinous tissues (P < 0.01 or 0.05), notably correlating with TNM stage, differentiation degree, clinical stage, and lymphatic metastasis (P < 0.01 or 0.05), but not gender, age, and LSCC growth sites (P > 0.05). The median survival of patients with positive KAI1/CD82 and MRP1/CD9 protein expression was longer than that of patients with negative protein expression (P < 0.01 or 0.05). KAI1/CD82 protein expression negatively correlated with MRP1/CD9 protein expression in LSCC (${\chi}^2$= 31.25, P < 0.01). Conclusion: KAI1/CD82 and MRP1/CD9 may jointly participate in the development of LSCC. They may serve as the markers for judging the infiltration, metastasis, and prognosis of LSCC.

Keywords

Laryngeal neoplasms;KAI1/CD82;MRP1/CD9;metastasis marker

Acknowledgement

Supported by : Natural Science Foundation

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