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MicroRNA-497 Suppresses Proliferation and Induces Apoptosis in Prostate Cancer Cells

  • Wang, Li (Department of Urology, Affiliated Hospital of Hebei, University of Engineering) ;
  • Li, Bo (Department of Urology, Affiliated Hospital of Hebei, University of Engineering) ;
  • Li, Lei (Department of General Surgery, Affiliated Hospital of Hebei, University of Engineering) ;
  • Wang, Te (School of Government of Beijing Normal University)
  • Published : 2013.06.30

Abstract

MicroRNAs (miRNAs) are a class of endogenously expressed small, non-coding, single-stranded RNAs that negatively regulate gene expression, mainly by binding to 3'- untranslated regions (3'UTR) of their target messenger RNAs (mRNAs), which cause blocks of translation and/or mRNA cleavage. Recently, miRNAprofiling studies demonstrated the microRNA-497 (miR-497) level to be down-regulated in all prostate carcinomas compared with BPH samples. The purpose of this study was to investigate the potential role of miR-497 in human prostate cancer. Proliferation, cell cycle and apoptosis assays were conducted to explore the potential function of miR-497 in human prostate cancer cells. Results showed that miR-497 suppressed cellular growth and initiated G0/G1 phase arrest of LNCaP and PC-3 cells. We also observed that miR-497 increased the percentage of apoptotic cells by increasing caspase-3/7 activity. Taken together, our results demonstrated that miR-497 can inhibit growth and induce apoptosis by caspase-3 activation in prostate cancer cells, which suggest its use as a potential therapeutic target in the future.

Keywords

MiR-497;prostate cancer;apoptosis;growth reduction

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