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A Comparative Study of Memory Improving Effects of Stachys Rhizome and Lycopi Rhizome on Scopolamine-induced Amensia in mice

시판 초석잠 기원식물의 기억력개선효과 비교연구

  • Received : 2013.08.15
  • Accepted : 2013.09.22
  • Published : 2013.09.30

Abstract

Objectives : The purpose of this study was to characterize the effect of the Ethanolic extract of Stachys sieboldii and Lycopus lucidus on the learning and memory impairments induced by scopolamine. Methods : The genetic difference of Stachys sieboldii and Lycopus lucidus were observed with RAPD analysis. The cognition-enhancing effect of Stachys sieboldii and Lycopus lucidus was investigated using a passive avoidance test, Y-maze test and the Morris water maze test in mice. Drug-induced amnesia was induced by treating animals with scopolamine (1 mg/kg, i.p.). Results : As a result of RAPD analysis, Stachys sieboldii and Lycopus lucidus Radix was found to be genetically different and The results of learning memory analysis showed that Stachys sieboldii extract-treated group (500 mg/kg, p.o.) and the tacrine-treated group (10 mg/kg, p.o.) significantly ameliorated scopolamine-induced amnesia based on the Passive avoidance Y-maze test and Water maze test. And these results are same manner in DPPH radical scavenger effect and Acetylcholineseterase inhibition effect. These results suggest that Stachys sieboldii extract maybe a useful cognitive impairment treatment, and its beneficial effects are depending on the origin plants. Conclusions : Commercially available Stachys sieboldii Radix consists of two original plant, one of them people misuse. To clarify the origin of the plant Memory tests were performed. These results suggest that 80% Ethanol extract of Stachys sieboldii showed significant anti-amnestic and cognitive-enhancing activities related to the memory processes, and these activities were parallel to treatment duration and dependent of the learning models.

Keywords

Stachys sieboldii;Memory enhancing;scopolamine

References

  1. Min SG. The Latest Psychiatry. Seoul : IlJoGak. 2006 : 203.
  2. Frank B, Gupta S. A review of antioxidants and Alzheimer's disease. Ann Clin Psychiatry. 2005 ; 17 : 269-86. https://doi.org/10.1080/10401230500296428
  3. Hermona S, Shlomo S. Acetylcholinesterase-new roles for an old actor. Nat Rev Neurosci. 2001 ; 2 : 294-302. https://doi.org/10.1038/35067589
  4. Talesa VN. Acetylcholine esterase in Alzheimer's disease. Mech Ageing Dev. 2001 ; 122 : 1961-9. https://doi.org/10.1016/S0047-6374(01)00309-8
  5. Kasa P, Rakonczay Z, Gulya K. The cholinergic system in Alzheimer's disease. Prog Neurobiol. 1997 ; 52 : 511-35. https://doi.org/10.1016/S0301-0082(97)00028-2
  6. Hong WS. Sophisticated translation version, su wen in Yellow Emperor's internal classic. Seoul : The Institute of Oriental Medicine. 1981 : 218.
  7. Park SK, Lee HJ, Kim HT. An experimental study of driental medicine on cure for dementia : the effect of Jowiseungcheongtang and Hyungbangjihwangtang on cure for aged rats. J Oriental Neropsychiatry 1998 ; 9(2) : 19-35.
  8. Lee DW, Shin GJ, Lee WC. A Comparative Consideration of Treatment on Dementia in Oriental and Occidental Medicine. J Dong Guk Oriental Medicine. 1995 ; 3 : 67-80.
  9. Lee CB. Illustrated flora of Korea. Seoul : Hyang Moon Sa. 1999 : 652.
  10. Yin J, Yang G, Wang S, Chen Y. Purification and determination of stachyose in Chinese artichoke (Stachys Sieboldii Miq.) by high-performance liquid chromatography with evaporative light scattering detection. Talanta. 2006 ; 70 : 208-12. https://doi.org/10.1016/j.talanta.2006.03.027
  11. Yamahara G. Studies on the Stachys sieboldii Miq. II. Anti-anoxia action and the active constituents. Med Mega. 1990 ; 110 : 932-5.
  12. Takeda Y, Fujita T, Satoh T, Kakegawa H. On the glycoside constituents of Stachys sieboldii Miq. and their effects on hyarulonidase activity. Yakugaku Zasshi. 1985 ; 105 : 955-9. https://doi.org/10.1248/yakushi1947.105.10_955
  13. Ryu BH. Park BG. Song SK. Antitumor effects of the hexane extract of Stachys sieboldii Miq. Kor J Biotechnol Bioeng. 2002 ; 17 : 520-4.
  14. Hayashi K, Nagamatsu T, Ito M, Yagita H, Suzuki Y. Acteoside, a component of Stachys sieboldii Miq, may be a promising antinephritic agent. Jpn J Pharmacol. 1996 ; 70 : 157-68. https://doi.org/10.1254/jjp.70.157
  15. Shin JH, Shin YW. Illustrated book of Medicinal herbs from Hyang Yak Jip Seong Bang. Daegu : KMU Press. 2006 : 123, 147.
  16. Woo ER, Piao MS. Antioxidative Constituents from Lycopus lucidus Arch. Pharm Res. 2004 ; 27 : 173-6.
  17. Shin SH, Sim Y, Kim YS, Chi HJ, Lee EB. Studies on Essential Oils of Lycopus lucidus Turcz. Kor J Pharmacogn. 1992 ; 23 : 29-33.
  18. Bak JP, Kim JB, Park JH, Yang YJ, Kim IS, Choung ES, Kang SC. Screening and Compound Isolation from Natural Plants for Anti-allergic Activity. J Korean Soc Appl Biol Chem. 2011 ; 54 : 367-75. https://doi.org/10.3839/jksabc.2011.058
  19. Kim MH. The antidiabetic properties of fractions of Lycopus lucidic Turcz in streptozotocin diabetic rats. Korean J Soc Food Sci. 2000 ; 16 : 644-51.
  20. Brinker ND. Inhibition of endocrine function by botanical agents. J Naturopathic Med. 1990 ; 1 : 10-5.
  21. Ryu BH, Kim SO. Effect of Methanol Extract of Stacys sieboldii MIQ on Acetylchloline Esterase and Monoamine Oxidase in Rat Brain. Korean J Food Nutr. 2004 ; 17(4) : 347-55.
  22. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958 ; 181 : 1199-200. https://doi.org/10.1038/1811199a0
  23. Ellman GL, Courtney KD, Andres Jr V, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961 ; 7 : 88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  24. Ingkaninan K, de Best CM, van der Heijden R, Hofte AJ, Karabatak B, Irth H, Tjaden UR, van der Greef J, Verpoorte R. High-performance liquid chromatography with on-line coupled UV, mass spectrometric and biochemical detection for identification of acetylcholinesterase inhibitors from natural products. J Chromatogr A. 2000 ; 872 : 61-73. https://doi.org/10.1016/S0021-9673(99)01292-3
  25. LeDoux JE. Emotional memory: in search of systems and synapses. Ann N Y Acad Sci. 1993 ; 702 : 149-57. https://doi.org/10.1111/j.1749-6632.1993.tb17246.x
  26. Sarter M, Bodewitz G, Stephens DN. Attenuation of scopolamine–induced impairment of spontaneous alternation behavior by antagonist but not inverse agonist and beta–carboline. Psycopharmacology. 1998 ; 94(4) : 491-5.
  27. Morris RG. Development of a water maze procedure for studying spatial learning in the rat. J Neurosci Meth. 1984 ; 11 : 47-60. https://doi.org/10.1016/0165-0270(84)90007-4
  28. Choi HY, Kim DW, Kim DE, Suh YB, Ham IH. The Relative Identification of C. officinale and L. chuanxiong by PCR- Mediated Fingerprinting. Kor J Herbology. 2005 ; 20(4) : 151-61.
  29. Coyle, JT. Puttfarcken P. Oxidative stress, glutamate and neurodegenerative disorders. Science. 1993 ; 262 : 689-95. https://doi.org/10.1126/science.7901908
  30. Hsieh HM, Wu WM, Hu ML. Genistein attenuates D-galactose-induced oxidative damage through decreased reactive oxygen species and NF-$\kappa{B}$ binding activity in neuronal PC12 cells. Life Sci. 2011 ; 88 : 82-8. https://doi.org/10.1016/j.lfs.2010.10.021
  31. Satoh T, Lipton SA. Redox regulation of neuronal survival mediated by electrophilic compounds. Trends Neurosci. 2007 ; 30 : 37-45. https://doi.org/10.1016/j.tins.2006.11.004
  32. Jung SH. The development and application of enzyme inhibitors. seoul : Shinil Pub. 1997 : 7-11.
  33. Choi CH. The effect of Gardenia Jasminoides Extract and geniposide ameliorate learning and memory impairment. Daegu Haany University. 2008.
  34. McEwen BS. Streess and hippocampal plasticity. Annu Rev Neurosci 1999 ; 22 : 105-22. https://doi.org/10.1146/annurev.neuro.22.1.105
  35. Tanabe F, Miyasaka N, Kubota T, Aso T. Estrogen and progesterone improve scopolamine-in duced impairment of spatial memory. J Med Dent Sci. 2004 ; 51 : 89-98.

Cited by

  1. Antioxidant Properties of Hot Water Extract of Lycopus lucidus Trucz Tubers vol.28, pp.1, 2017, https://doi.org/10.7856/kjcls.2017.28.1.103
  2. Nutritional Components and Antioxidant Activities of Various Stachys Sieboldii Miq Parts vol.28, pp.2, 2017, https://doi.org/10.7856/kjcls.2017.28.2.203

Acknowledgement

Supported by : 지식경제부