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Changes in Microbial Diversity, Methanogenesis and Fermentation Characteristics in the Rumen in Response to Medicinal Plant Extracts

  • Kim, Eun Tae (Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science)) ;
  • Moon, Yea Hwang (Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology) ;
  • Min, Kwan-Sik (Animal Biotechnology, GSBIT, Hankyong National University) ;
  • Kim, Chang-Hyun (Department of Animal Life and Environment Science, Hankyong National University) ;
  • Kim, Sam Churl (Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science)) ;
  • Ahn, Seung Kyu (Sancheong Oriental Medicinal Herb Institute) ;
  • Lee, Sung Sill (Division of Applied Life Science, Graduate School of Gyeongsang National University (Institute of Agriculture and Life Science))
  • Received : 2013.01.29
  • Accepted : 2013.06.13
  • Published : 2013.09.01

Abstract

This study evaluated the in vitro effect of medicinal plant extracts on ruminal methanogenesis, four different groups of methanogens and ruminal fermentation characteristics. A fistulated Holstein cow was used as a donor of rumen fluid. Licorice and mugwort extracts (Glycyrrhiza uralensis and Artemisia capillaris, 0.5% and 1% of total substrate DM, respectively), previously used as folk remedies, were added to an in vitro fermentation incubated with buffered-rumen fluid. Total gas production in Glycyrrhiza uralensis extract treatment was not significantly different between treatments (p<0.05) while total gas production in the Artemisia capillaris extract treatment was lower than that of the control. Artemisia capillaris extract and Glycyrrhiza uralensis extract reduced $CH_4$ emission by 14% (p<0.05) and 8% (p<0.05), respectively. Ciliate-associated methanogens population decreased by 18% in the medicinal plant extracts treatments. Medicinal plant extracts also affected the order Methanobacteriales community. Methanobacteriales diversity decreased by 35% in the Glycyrrhiza uralensis extract treatment and 30% in the Artemisia capillaris extract treatment. The order Methanomicrobiales population decreased by 50% in the 0.5% of Glycyrrhiza uralensis extract treatment. These findings demonstrate that medicinal plant extracts have the potential to inhibit in vitro ruminal methanogenesis.

Keywords

Methane Emission;Medicinal Plant Extracts;Real-time PCR;Relative Quantification;Ruminal Methanogenesis

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