The Toxicological Effects of Ahnjon-Yichun-Tang in Pregnant Rats and Fetuses

안존이천탕 추출물이 흰쥐의 모체 및 태자에 미치는 영향

  • Kim, Bum Hoi (Department of Anatomy, College of Oriental Medicine and Research Institute of Oriental Medicine, Dong-Eui University)
  • 김범회 (동의대학교 한의과대학 한의학과,동의대학교 한의학연구소)
  • Received : 2013.04.10
  • Accepted : 2013.08.13
  • Published : 2013.08.31


The objective of this study was to characterize the adverse effects of Ahnjon-Yichun-Tang during early pregnancy. Following successful mating, female Sprague-Dawley rats were given Ahnjon-Yichun-Tang(AYT) extract by oral administration daily with dose of 150mg (n=10), 300mg(n=10), 450mg(n=10) for 20 days of pregnancy. The rats in Control group(n=10) were orally administrated with Saline. All pregnant rats of Ahnjon-Yichun-Tang-treated and Control groups were sacrificed on day 20 of pregnancy. The pregnancy outcome was determined and the internal and reproductive organs of pregnant rat were observed. The fetuses were examined for the presence of various developmental toxic endpoints and stained with alcian blue and alizarin red S, and observed skeletal malformations. The results obtained in this study represent that there is no significant changes between Control and Ahnjon-Yichun-Tang-treated groups in body weight, organ weight, blood chemistry values, hematological values and pregnancy indexes of pregnant rat. The skeletal malformation of fetus was not observed as well. These results suggest that oral administration of Ahnjon-Yichun-Tang does not produce either maternal or developmental toxicity.


Supported by : 동의대학교


  1. Westfall RE. Herbal heal in ginpregnancy: women's experiences. J Herb Pharmacother. 2003;3(4):17-39
  2. Fakeye TO, Adisa R, Musa IE. Attitude and use of herbal medicines among pregnant women in Nigeria. BMC Complement Altern Med. 2009 Dec 31;9:53.
  3. 이현희, 김윤상, 임은미. 姙娠중 한약복용 환자에 관한 논문 23례 분석. 대한한방부인과학회지. 2007;20(3):185-199.
  4. 한의부인과학교재편찬위원회. 한의부인과학 하. 서울: 도서출판 정담. 2001:97-100.126.151.
  5. 조현주, 임은미. 습관성 유산 치료 14례 임상보고. 2005;18(2):159-168.
  6. 김성란. 安尊二天湯을 中心으로 한 胎漏및 胎動不安의 文獻的考察. 대전대학교 한의학연구소 논문집. 1998;13:609-624.
  7. 박귀례, 신재호, 김판기, 이유미, 장성재. 방사선 조사 인삼이 랫드의 기형유발에 미치는 영향에 관한 연구. 한국독성학회지. 2001; 17(1):27-32.
  8. 한용주, 신헌태, 이선동. 보중익기탕과 귀비탕 투여가 임신랫드의 모체 및 태자에 미치는 연구. 대한예방한의학회지. 2010;14(2):91-104.
  9. 한상백. 십전대보탕과 육미지황탕이 실험동물의 모체와 태자에 미치는 생식독성학적 연구. 상지대학교대학원. 2008.
  10. 전성진. 사물탕 용량별 투여가 랫드의 모체와 태자에 미치는 영향. 상지대학교대학원, 2009.
  11. Chen FP, Kung YY, Chen YC, Jong MS, Chen TJ, Chen FJ, Hwang SJ. Frequency and pattern of Chinese herbal medicine prescriptions for chronic hepatitis in Taiwan. J Ethnopharmacol. 2008;117(1):84-91.
  12. McRae CA, Agarwal K, Mutimer D, Bassendine MF. Hepatitis associated with Chinese herbs. Eur J Gastroenterol Hepatol. 2002; 14(5):559-562.
  13. Stedman C. Herbal hepatotoxicity. Semin Liver Dis. 2002;22(2):195-206.
  14. 박병욱, 허금정, 고흥, 이은. 한약과 민간약물의 독성 및 부작용에 대한 고찰. 대한한방내과학회지. 2002;23(2):222-227.
  15. 윤영주. 한약이 간손상을 유발할 가능성이 있는가 : 한약 관련 간손상에 관한 국내 연구의 체계적 고찰. 경희대학교 박사학위논문. 2009.
  16. Ozer J, Ratner M, Shaw M, Bailey W, Schomaker S. The current state of serum biomarkers of hepatotoxicity. Toxicology. 2008 Mar 20;245(3):194-205.
  17. Niemela O, Alatalo P. Biomarkers of alcohol consumption and related liver disease. Scand J Clin Lab Invest. 2010 Sep;70(5):305-312.
  18. Herlong HF. Approach to the patient with abnormal liver enzymes. Hosp Pract(Off Ed). 1994 Nov 15;29(11):32-8.
  19. 식품의약품안전청. 의약품 등의 독성시험 기준 해설서. 1999:1-254.
  20. Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, Van Rompay M, Kessler RC. Trends in alternative medicine use in the United States,1990-1997: results of a followup national survey. JAMA.1998 Nov 11; 280(18):1569-1575.
  21. Banfi G, Colombini A, Lombardi G, Lubkowska A. Metabolic markers in sports medicine. Adv Clin Chem. 2012;56:1-54
  22. Mapanga RF, Musabayane CT. The renal effects of blood glucose-lowering plantderived extracts in diabetes mellitus-an overview. Ren Fail. 2010 Jan;32(1):132-8
  23. Lyman JL. Blood urea nitrogen and creatinine. Emerg Med Clin North Am. 1986 May;4(2):223-233.
  24. Edelstein CL. Biomarkers of acute kidney injury. Adv Chronic Kidney Dis. 2008 Jul; 15(3):222-234.
  25. Poole JH, Thorsen MS. Acute renal failure in pregnancy. MCN Am J Matern Child Nurs. 1999 Mar-Apr;24(2):66-72.
  26. Alexopoulos E, Tampakoudis P, Bili H, Mantalenakis S. Acute uric acid nephropathy in pregnancy. Obstet Gynecol. 1992 Sep;80(3 Pt 2):488-9.
  27. McCredie J, Loewenthal J. Pathogenesis of congenital malformations. An hypothesis. Am J Surg.1978:135(3):293-297.
  28. O'Halloran K, Spickett JT. The interaction of lead exposure and pregnancy. Asia Pac J Public Health. 1992;6(2):35-39.