- Volume 26 Issue 4
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Separation and growth monitoring of oyster mushroom spores using gravitational field-flow fractionation (GrFFF)
중력 장-흐름 분획법을 이용한 느타리버섯 포자의 분리 및 성장 모니터링
- Kim, Sun Tae (Department of Chemistry, Hannam University) ;
- Ji, Eun Young (Department of Chemistry, Hannam University) ;
- Lee, Jae Chan (Jung-do mushroom laboratory) ;
- Lee, Seungho (Department of Chemistry, Hannam University)
- Received : 2013.07.25
- Accepted : 2013.08.03
- Published : 2013.08.25
Gravitational field-flow fractionation (GrFFF) is a separation technique that utilizes earth's gravity as the external field. GrFFF is a convenient tool for the size and/or density-based separation of micron-sized particles of various origins. In this study, GrFFF was employed for size-based separation of oyster mushroom spores. Oyster mushroom spores have smooth surface and are of cylindrical to narrow kidney-shapes with 5 to 12 im in longer dimension and 3 to 4 im in shorter dimension, as was confirmed by optical microscope (OM). GrFFF conditions were optimized for separation and characterization of spores by varying the channel flow rate from 0.5 to 1 mL/min. During the GrFFF elution of the spores, 3 fractions were collected to confirm the growth of oyster mushroom spore. The collected fractions were incubated for 30 days in water to examine the influence of the size on the growth of the spores. Results suggested that the oyster mushroom spores collected at the middle part of the GrFFF fractogram grew faster than those collected at the beginning or at the end of the fractogram.
gravitational field-flow fractionation (GrFFF);oyster mushroom spore;Size distribution
Supported by : Hannam University
- I. C. Jung and J. S. Lee, J Korean Society for Hygenic Science, 5(1), 19-24 (1999).
- Y. Yoshioka, T. Ikekawa, M. Noda and F. Fukuoka, Chem. Pharm. Bull., 20(6), 1175-1180 (1972). https://doi.org/10.1248/cpb.20.1175
- H. Wang, J. Gao, and T. B. Ng, Biochem. Biophys. Res. Commun., 275(3), 810-816 (2000). https://doi.org/10.1006/bbrc.2000.3373
- P. Bobek, L. Ozdín and S. Galbavý, Nutrition, 14(3), 282-286 (1998). https://doi.org/10.1016/S0899-9007(97)00471-1
- S.-t. Chang and P. G. Miles, 'Edible mushrooms and their cultivation', CRC Press, 1989.
- H. S. Choi and H. H. Shin, Mycologia, 90(4), 674-679 (1998). https://doi.org/10.2307/3761226
- P. Stamets, 'Growing Gourmet and Medicinal Mushrooms', Ten Speed Press, 2000.
- R. Scrase, Mycologist, 9(2), 53-56 (1995). https://doi.org/10.1016/S0269-915X(09)80207-3
- S. Rasouli, E. Assidjo, T. Chianéa and P. J. P. Cardot, J. Chromatogr. B, 754(1), 11-21 (2001). https://doi.org/10.1016/S0378-4347(00)00581-8
- P. Reschiglian, D. Melucci, G. Torsi and A. Zattoni, Chromatographia, 51(1-2), 87-94 (2000). https://doi.org/10.1007/BF02490701
- C. Contado, P. Reschiglian, S. Faccini, A. Zattoni and F. Dondi, J. Chromatogr. A, 871(1-2), 449-460 (2000). https://doi.org/10.1016/S0021-9673(99)01191-7
- R. Sanz, L. Puignou, M. T. Galceran, P. Reschiglian, A. Zattoni and D. Melucci, Anal. Bioanal. Chem., 379(7-8), 1068-1075 (2004).
- E. Urbankova, A. Vacek and J. Chmelik, J. Chromatogr. B, 687(2), 449-452 (1996). https://doi.org/10.1016/S0378-4347(96)00249-6
- P. Reschiglian, A. Zattoni, B. Roda, S. Casolari, M. H. Moon, J. Lee, J. Jung, K. Rodmalm and G. Cenacchi, Anal. Chem., 74(19), 4895-4904 (2002). https://doi.org/10.1021/ac020199t
- S. B. Cho, In 'Oyster Mushroom Cultivation', pp 1-3, MushWorld, Seoul, Korea, 2004.
- S. T. Kim, S. Y. Seo and S. Lee, Anal. Sci. Technol., 24(4), 249-255 (2011). https://doi.org/10.5806/AST.2011.24.4.249
- M. R. Park, D. Y. Kang, J. Chmelik, N. Kang, J. S. Kim and S. Lee, J. Chromatogr. A, 1209(1-2), 206-211 (2008). https://doi.org/10.1016/j.chroma.2008.09.014