Analytical Science and Technology (분석과학)
- Volume 26 Issue 4
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- Pages.221-227
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- 2013
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- 1225-0163(pISSN)
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- 2288-8985(eISSN)
DOI QR Code
Validation of LC-MS/MS method for determination of ginsenoside Rg1 in human plasma
인체 혈장 중 Ginsenoside Rg1의 정량을 위한 LC-MS/MS 분석법 검증
- Kim, Yunjeong (Biomedical Research Institute of Chonbuk National University Hospital) ;
- Han, Song-Hee (Biomedical Research Institute of Chonbuk National University Hospital) ;
- Jeon, Ji-Young (Biomedical Research Institute of Chonbuk National University Hospital) ;
- Hwang, Min-Ho (Biomedical Research Institute of Chonbuk National University Hospital) ;
- Im, Yong-Jin (Biomedical Research Institute of Chonbuk National University Hospital) ;
- Lee, Sun Young (Biomedical Research Institute of Chonbuk National University Hospital) ;
- Chae, Soo-Wan (Biomedical Research Institute of Chonbuk National University Hospital) ;
- Kim, Min-Gul (Biomedical Research Institute of Chonbuk National University Hospital)
- 김윤정 (전북대학교병원 의생명연구원) ;
- 한송희 (전북대학교병원 의생명연구원) ;
- 전지영 (전북대학교병원 의생명연구원) ;
- 황민호 (전북대학교병원 의생명연구원) ;
- 임용진 (전북대학교병원 의생명연구원) ;
- 이선영 (전북대학교병원 의생명연구원) ;
- 채수완 (전북대학교병원 의생명연구원) ;
- 김민걸 (전북대학교병원 의생명연구원)
- Received : 2013.03.06
- Accepted : 2013.06.10
- Published : 2013.08.25
Abstract
A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the investigation of the ginsenoside Rg1 in human plasma. After addition of internal standard (digoxin), plasma was diluted with acetone and methanol (80:20), the supernatant was concentrated and analyzed by LC-MS/MS. The optimal chromatographic separation was achieved on an Agilent Eclipse XDB-C18 column (
Keywords
ginsenoside Rg1;LC-MS/MS;validation;human plasma
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