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Validation of LC-MS/MS method for determination of ginsenoside Rg1 in human plasma

인체 혈장 중 Ginsenoside Rg1의 정량을 위한 LC-MS/MS 분석법 검증

  • Kim, Yunjeong (Biomedical Research Institute of Chonbuk National University Hospital) ;
  • Han, Song-Hee (Biomedical Research Institute of Chonbuk National University Hospital) ;
  • Jeon, Ji-Young (Biomedical Research Institute of Chonbuk National University Hospital) ;
  • Hwang, Min-Ho (Biomedical Research Institute of Chonbuk National University Hospital) ;
  • Im, Yong-Jin (Biomedical Research Institute of Chonbuk National University Hospital) ;
  • Lee, Sun Young (Biomedical Research Institute of Chonbuk National University Hospital) ;
  • Chae, Soo-Wan (Biomedical Research Institute of Chonbuk National University Hospital) ;
  • Kim, Min-Gul (Biomedical Research Institute of Chonbuk National University Hospital)
  • 김윤정 (전북대학교병원 의생명연구원) ;
  • 한송희 (전북대학교병원 의생명연구원) ;
  • 전지영 (전북대학교병원 의생명연구원) ;
  • 황민호 (전북대학교병원 의생명연구원) ;
  • 임용진 (전북대학교병원 의생명연구원) ;
  • 이선영 (전북대학교병원 의생명연구원) ;
  • 채수완 (전북대학교병원 의생명연구원) ;
  • 김민걸 (전북대학교병원 의생명연구원)
  • Received : 2013.03.06
  • Accepted : 2013.06.10
  • Published : 2013.08.25

Abstract

A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the investigation of the ginsenoside Rg1 in human plasma. After addition of internal standard (digoxin), plasma was diluted with acetone and methanol (80:20), the supernatant was concentrated and analyzed by LC-MS/MS. The optimal chromatographic separation was achieved on an Agilent Eclipse XDB-C18 column ($4.6{\times}150mm$, $5{\mu}m$) with a mobile phase of 0.1% formic acid in water and 0.1% formic acid in methanol at a flow rate of 0.9 mL/min gradient mode. The standard calibration curve for ginsenoside Rg1 was linear ($r^2=0.9995$) over the concentration range 1~500 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of ginsenoside Rg1 was lower than 7.53% (correlation of variance, CV), and accuracy exceeded 98.28%. This LC-MS/MS assay of ginsenoside Rg1 in human plasma is applicable for quantifying in the pharmacokinetic study.

Keywords

ginsenoside Rg1;LC-MS/MS;validation;human plasma

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