- Volume 19 Issue 2
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The Complete Nucleotide Sequence of a Korean Isolate Bean yellow mosaic virus from Freesia sp. and Comparison to Other Potyviruses
- Choi, Sun-Hee (Department of Horticulture and Landscape, College of Natural Science, Seoul Women's University) ;
- Yoon, Ju-Yeon (Department of Horticulture and Landscape, College of Natural Science, Seoul Women's University) ;
- Ryu, Ki-Hyun (Department of Horticulture and Landscape, College of Natural Science, Seoul Women's University) ;
- Choi, Seung-Kook (Virology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration)
- Received : 2013.04.01
- Accepted : 2013.05.02
- Published : 2013.06.30
Bean yellow mosaic virus (BYMV; genus Potyvirus, family Potyviridae) causes severe losses to various legume species and a number of non-legume species, particularly freesia plants. In a survey of virus diseases in Gyeonggi province, Korea, BYMV isolates were identified from many cultivated freesia species. Here, we determined the complete nucleotide sequences of a BYMV freesia isolate (BYMV-Fr; accession number FJ492961). BYMV-Fr genome consists of 9,545 nucleotides (nt) excluding the poly (A) tail and encodes 3,057 amino acid (aa), with an AUG start and UAG stop codon, containing one open reading frame typical of a potyvirus polyprotein. The polyprotein of BYMV-Fr was divided to ten proteins and the cleavage sites of each protein were determined. The coat protein (CP) and polyprotein of BYMV-Fr were compared at the aa level with those of the previously reported 4 BYMV isolates. BYMV-Fr shared 90.1 to 97.1 and 91.0 to 92.5% at the CP and polyprotein homology. Interestingly, BYMV-Fr showed identities of a lower level at the nt level of 5' noncoding region (61.4 to 67.6%) and at the aa level of P1 (71.4 to 72.8%), comparing with four BYMV isolates. Based on the aa sequence diversity of CP and polyprotein, phylogenetic analysis with the four BYMV isolates showed two distinct groups and BYMV-Fr and most BYMV isolates were most closely related to the clover yellow vein virus among 52 potyviruses. To our knowledge, this is the first report of the complete genome sequence of BYMV freesia strain.
Bean yellow mosaic virus;Freesia;Genetic diversity;Potyvirus;RT-PCR;Sequence analysis
Supported by : Rural Development Administration
- Allison, R., Johnston, R. E. and Dougherty, W. G. 1986. The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9−20. https://doi.org/10.1016/0042-6822(86)90425-3
- Bos, L. 1970. Descriptions of Plant Viruses, Bean yellow mosaic virus, CMI/AAB, UK.
- Brunt, A. A. 1995. Virus and Virus-like Diseases of Bulb and Flower Crops. pp. 274-280. John Wiley & Sons, UK.
- Chang, C. A., Hiebert, E. and Purcifull, D. E. 1988a. Analysis of in vitro translation of Bean yellow mosaic virus RNA: Inhibition of proteolytic processing by antiserum to the 49K nuclear inclusion protein. J. Gen. Virol. 69: 1117−1122. https://doi.org/10.1099/0022-1317-69-5-1117
- Chang, C. A., Hiebert, E. and Purcifull, D. E. 1988b. Purification, characterization, and immunological analysis of nuclear inclusions induced by bean yellow mosaic and clover yellow vein potyviruses. Phytopathology 78: 1266−1275. https://doi.org/10.1094/Phyto-78-1266
- Derks, A. F. L. M., Vink-van den Abeele, J. L. and Muller, P. J. 1980. Bean yellow mosaic virus in some iridaceous plants. Acta Hort. 110: 31−37.
- Dougherty, W. G., Cary, S. M. and Parks, T. D. 1989. Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. Virology 171: 356−364. https://doi.org/10.1016/0042-6822(89)90603-X
- Gal-On, A. 2000. A point mutation in the FRNK motif of the potyvirus helper component-protease gene alters symptom expression in cucurbits and elicits protection against the severe homologous virus. Phytopathology 90: 467−473. https://doi.org/10.1094/PHYTO.2000.90.5.467
- Ganesh-Selvaraj, D. G., Pokorný, R. and Holková, L. 2009. Variability of Bean yellow mosaic virus isolates in the Czech Republic. Acta Virol. 53: 277−280. https://doi.org/10.4149/av_2009_04_277
- Guyatt, K. J., Proll, D. F., Menssen, A. and Davidson, A. J. 1996. The complete nucleotide sequence of bean yellow mosaic potyvirus RNA. Arch. Virol. 141: 1231−1246. https://doi.org/10.1007/BF01718827
- Hammond, J. and Hammon, R. W. 2003. The complete nucleotide sequence of isolate BYMV-GDD of Bean yellow mosaic virus, and comparison to other potyviruses. Arch. Virol. 148: 2461−2470. https://doi.org/10.1007/s00705-003-0185-7
- Kumar, Y., Hallan, V. and Zaidi, A. A. 2009. Identification and characterization of Bean yellow mosaic virus infecting freesia. J. Plant Biochem. Biotech. 18: 253−255. https://doi.org/10.1007/BF03263331
- Lopez-Moya, J. J., Wang, R. Y. and Pirone, T. P. 1999. Context of the coat protein DAG motif affects potyviurs transmissibility by aphids. J. Gen. Virol. 80: 3281−3288. https://doi.org/10.1099/0022-1317-80-12-3281
- Magie, R. O. and Poe, S. L. 1972. Disease and pest associates of bulbs and plants. In: The World of Gladiolus, pp. 155-167. Edgewood Press, USA.
- Parrella, G. and Lanave, C. 2009. Identification of a new pathotype of Bean yellow mosaic virus (BYMV) infecting blue passion flower and some evolutionary characteristics of BYMV. Arch. Virol. 154: 1689-1694. https://doi.org/10.1007/s00705-009-0485-7
- Randle, R. W., Davies, C., Gibbs, A. J. and Hatta, T. 1980. Amino acid composition of capsid protein as a taxonomic criterion for classifying the atypical S strain of Bean yellow mosaic virus. Aust. J. Biol. Sci. 33: 243-254.
- Shukla, D. D., Strike, P. M., Tracy, S. L., Gough, K. H. and Ward, C. W. 1988. The N and C terminal of the coat protein Potyviruses are surface located and the N-terminus contains major virus specific epitopes. J. Gen. Virol. 69: 1497-1508. https://doi.org/10.1099/0022-1317-69-7-1497
- Shukla, D. D., Ward, C. W. and Brunt, A. A. 1994. The Potyviridae. CAB International, UK, 516 pp.
- Takahashi, T., Uyeda, I., Ohshima, K. and Shikata, E. 1990. Nucleotide sequence of the capsid protein gene of Bean yellow mosaic virus chlorotic spot strain. J. Fac. Agr. Hokkaido Univ. 64: 152-163.
- Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731-2739. https://doi.org/10.1093/molbev/msr121
- Urcuqui-Inchima, S., Haenni, A. and Bernardi, F. 2001. Potyvirus proteins: a wealth of functions. Virus Res. 74: 157−175. https://doi.org/10.1016/S0168-1702(01)00220-9
- Vaira, A. M., Lisa, V., Costantini, A., Masenga, V., Rapetti, S. and Milne, R. G. 2006. Ophioviruses infecting ornamentals and a probable new species associated with a severe disease in freesia. Acta Hort. 722: 191−199.
- Vance, V. B., Moore, D., Turpen, T. H., Bracker, A. and Hollowell, V. C. 1992. The complete nucleotide sequence of pepper mottle virus genomic RNA: comparison of the encoded polyprotein with those of other sequenced potyviruses. Virology 191: 19−30. https://doi.org/10.1016/0042-6822(92)90162-I
- Wada, Y., Iwai, H., Ogawa, Y. and Arai, K. 2000. Comparison of pathogenicity and nucleotide sequences of 30-terminal regions of Bean yellow mosaic virus isolate from gladiolus. J. Gen. Plant Pathol. 66: 345−352. https://doi.org/10.1007/PL00012976
- Wang, L. 2006. Freesia. In: Flower Breeding and Genetics, ed. N. O. Anderson, pp. 665-693. Springer, The Netherlands.
- Wylie, S. J., Kueh, J., Welsh, B., Smith, L. J., Jones, M. G. K. and Jones, R. A. C. 2002. A non-aphid-transmissible isolate of bean yellow mosaic potyvirus has an altered NAG motif in its coat protein. Arch. Virol. 147: 1813-1820. https://doi.org/10.1007/s00705-002-0846-y
- Wylie, S. J., Coutts, B. A., Jones, M. G. K. and Jones, R. A. C. 2008. Phylogenetic analysis of Bean yellow mosaic virus isolates from four continents: Relationship between the seven groups found and their hosts and origins. Plant Dis. 92: 1596-1603. https://doi.org/10.1094/PDIS-92-12-1596
- Observation of Mitotic and Cytological Effects Induced by Bean Yellow Mosaic Virus in Affecting Leguminous Plants vol.11, pp.3, 2015, https://doi.org/10.3923/ijv.2015.123.132
- Nucleotide Sequence of Capsid Protein Gene of Bean yellow mosaic potyvirus in Bean Plants from Al-Makhwah Governorate, Saudi Arabia vol.11, pp.2, 2015, https://doi.org/10.3923/ijv.2015.41.53