Effect of Xeroderma Pigmentosum Complementation Group F Polymorphisms on Gastric Cancer Risk and Associations with H.pylori Infection

  • Zhang, Ji-Shun (Department of Gastroenterology, the Affiliated Beijing Chaoyang Hospital, Capital Medical University) ;
  • Zhang, Chuan (Department of Gastroenterology, the Affiliated Beijing Chaoyang Hospital, Capital Medical University) ;
  • Yan, Xue-Yan (Department of Gastroenterology, the Affiliated Beijing Chaoyang Hospital, Capital Medical University) ;
  • Yuan, Zhi-Fang (Department of Gastroenterology, the Affiliated Beijing Chaoyang Hospital, Capital Medical University) ;
  • Duan, Zhuo-Yang (Department of Gastroenterology, the Affiliated Beijing Chaoyang Hospital, Capital Medical University) ;
  • Gao, Hui (Department of Gastroenterology, the Affiliated Beijing Chaoyang Hospital, Capital Medical University)
  • 발행 : 2013.03.30


We conducted a hospital case-control study by genotyping four potential functional single nucleotide polymorphisms (SNPs) to assess the association of Xeroderma pigmentosum complementation group F (XPF) with gastric cancer susceptibility, and role of XPF polymorphisms in combination with H.pylori infection in risk definition. A total of 331 patients with gastric cancer and 355 controls were collected. Four SNPs of XPF, rs180067, rs1799801, rs2276466 and rs744154, were genotyped by Taqman real-time PCR method with a 7900 HT sequence detector system. The gastric cancer patients were more likely to have smoking habit, a family history of cancer and H.pylori infection. We did not find any significant difference in the genotype distributions of XPF rs180067, rs1799801, rs2276466 and rs744154 between cases and controls. However, multivariate logistic analysis showed a non-significant decreased risk in patients carrying rs180067 G allele, rs1799801 T allele or rs2276466 T allele genotypes. A non-significant increased risk of gastric cancer was found in individuals carrying the rs744154 GG genotype. Stratification by H.pylori infection and smoking was not significantly different in polymorphisms of XPF rs180067, rs1799801, rs2276466 and rs744154. The four XPF SNPs did not show significant interaction with H.pylori infection and smoking status (P for interaction was 0.35 and 0.18, respectively). Our study indicated that polymorphisms in rs180067, rs1799801, rs2276466 and rs744154 may affect the risk of gastric cancer but further large sample size studies are needed to validate any association.


연구 과제 주관 기관 : Capital Medical University


  1. Agalliu I, Kwon EM, Salinas CA, et al (2010). Genetic variation in DNA repair genes and prostate cancer risk: results from a population-based study. Cancer Causes Control, 21, 289-300.
  2. Chang JS, Wrensch MR, Hansen HM, et al (2008). Nucleotide excision repair genes and risk of lung cancer among San Francisco Bay Area Latinos and African Americans. Int J Cancer, 123, 2095-104.
  3. Cleaver JE (2000). Common pathways for ultraviolet skin carcinogenesis in the repair and replication defective groups of xeroderma pigmentosum. J Dermatol Sci, 23, 1-11.
  4. Crew KD, Gammon MD, Terry MB, et al (2007). Polymorphisms in nucleotide excision repair genes, polycyclic aromatic hydrocarbon-DNA adducts, and breast cancer risk. Cancer Epidemiol Biomarkers Prev, 16, 2033-41.
  5. De Silva IU, McHugh PJ, Clingen PH, Hartley JA (2000). Defining the roles of nucleotide excision repair and recombination in the repair of DNA interstrand cross-links in mammalian cells. Mol Cell Biol, 20, 7980-90.
  6. Friedberg EC (2001). How nucleotide excision repair protects against cancer. Nat Rev Cancer, 1, 22-33.
  7. Friedberg EC, Bond JP, Burns DK, et al (2000). Defective nucleotide excision repair in XPC mutant mice and its association with cancer predisposition. Mutat Res, 459, 99-108.
  8. Ghoshal UC, Tiwari S, Dhingra S, et al (2008). Frequency of Helicobacter pylori and CagA antibody in patients with gastric neoplasm and controls: the Indian enigma. Dig Dis Sci, 53, 1215-22.
  9. Ghoshal UC, Tripathi S, Ghoshal U (2007). The Indian enigma of frequent H. pylori infection but infrequent gastric cancer:is the magic key in Indian diet, host's genetic make up, or friendly bug? Am J Gastroenterol, 102, 2113-4.
  10. Gil J, Ramsey D, Stembalska A, Karpinski P, et al. (2011). The C/A polymorphism in intron 11 of the XPC gene plays a crucial role in the modulation of an individual's susceptibility to sporadic colorectal cancer. Mol Biol Rep, 39, 527-34.
  11. Graham DY, Adam E, Reddy GT, et al (1991). Seroepidemiology of Helicobacter pylori infection in India; comparison of developing and developing countries. Dig Dis Sci, 36, 1084-8.
  12. He J, Qiu LX, Wang MY, et al (2012). Polymorphisms in the XPG gene and risk of gastric cancer in Chinese populations. Hum Genet, 131, 1235-44.
  13. He J, Xu Y, Qiu LX, et al (2012). Polymorphisms in ERCC1 and XPF genes and risk of gastric cancer in an eastern Chinese population. PLoS One, 7, e49308.
  14. International Agency for Research on Cancer (2008). Globocan 2008: Stomach Cancer incidence, Mortality and Prevalence Worldwide in 2008. IARC.
  15. Mueser TC, Nossal NG, Hyde CC (1996). Structure of bacteriophage T4 RNase H, a 50 to 30 RNA-DNA and DNADNA exonuclease with sequence similarity to the RAD2 family of eukaryotic proteins. Cell, 85, 1101-12.
  16. Parsonnet J, Friedman GD, Orentreich N, et al (1997). Risk for gastric cancer in people with CagA positive or CagA negative Helicobacter pylori infection. Gut, 40, 297-301.
  17. Wood RD, Mitchell M, Sgouros J, et al (2001). Human DNA repair genes. Science, 291, 1284-9.
  18. Yu H, Liu Z, Huang YJ, et al (2012). Association between single nucleotide polymorphisms in ERCC4 and risk of squamous cell carcinoma of the head and neck. PLoS One, 7, e41853.
  19. Zhou RM, Niu CX, Wang N, et al (2012). ERCC1 gene +262A/C polymorphism associated with risk of gastric cardiac adenocarcinoma in nonsmokers. Arch Med Res, 43, 67-74.

피인용 문헌

  1. XPF-673C>T Polymorphism Effect on the Susceptibility to Esophageal Cancer in Chinese Population vol.9, pp.4, 2014,
  2. Association of XPF Levels and Genetic Polymorphism with Susceptibility to Ischemic Stroke vol.59, pp.1, 2016,