- Volume 14 Issue 3
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Quantification of Her-2/Neu Gene in Breast Cancer Patients using Real Time-Polymerase Chain Reaction (Q-PCR) and Correlation with Immunohistochemistry Findings
- Abdul Murad, Nor Azian (UKM Medical Molecular Biology Institute) ;
- Razak, Zuraini Abdul (UKM Medical Molecular Biology Institute) ;
- Hussain, Rosniza Muhammmad (UKM Medical Molecular Biology Institute) ;
- Syed Hussain, Sharifah Noor Akmal (Department of Pathology, UKM Medical Centre, Universiti Kebansaan Malaysia) ;
- Ching Huat, Clarence Ko (Department of Pathology, UKM Medical Centre, Universiti Kebansaan Malaysia) ;
- Siti Aishah, Che Md. Ali (Department of Pathology, UKM Medical Centre, Universiti Kebansaan Malaysia) ;
- Abdullah, Norlia (Department of Surgery, UKM Medical Centre, Universiti Kebansaan Malaysia) ;
- Muhammad, Rohaizak (Department of Surgery, UKM Medical Centre, Universiti Kebansaan Malaysia) ;
- Ibrahim, Naqiyah (Department of Surgery, UKM Medical Centre, Universiti Kebansaan Malaysia) ;
- Jamal, Rahman (UKM Medical Molecular Biology Institute)
- Published : 2013.03.30
Background: HER-2/neu is a proto-oncogene that encodes a transmembrane tyrosine kinase growth factor which is crucial for stimulating growth and cellular motility. Overexpression of HER-2/neu is observed in 10-35% of human breast cancers and is associated with pathogenesis, prognosis as well as response to therapy. Given the imperative role of HER-2/neu overexpression in breast cancer, it is important to determine the magnitude of amplification which may facilitate a better prognosis as well as personalized therapy in affected patients. In this study, we determined HER-2/neu protein expression by immunohistochemistry (IHC) concurrently with HER-2/neu DNA amplification by quantitative real time-polymerase chain reaction (Q-PCR). Materials and Methods: A total of 53 paired tissue samples from breast cancer patients were frozen-sectioned to characterize the tumour and normal tissues. Only tissues with 80% tumour cells were used in this study. For confirmation, Q-PCR was used to determine the HER-2/neu DNA amplification. Results: We found 20/53 (37.7%) of the tumour tissues to be positive for HER-2/neu protein overexpression using IHC. Out of these twenty, only 9/53 (17%) cases were in agreement with the Q-PCR results. The concordance rate between IHC and Q-PCR was 79.3%. Approximately 20.7% of positive IHC cases showed no HER-2/neu gene amplification using Q-PCR. Conclusion: In conclusion, IHC can be used as an initial screening method for detection of the HER-2/neu protein overexpression. Techniques such as Q-PCR should be employed to verify the IHC results for uncertain cases as well as determination of HER-2/neu gene amplification.
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