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Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

  • Khalilpour, Akbar (Institute for Research in Molecular Medicine, Universiti Sains Malaysia) ;
  • Santhanam, Amutha (Institute for Research in Molecular Medicine, Universiti Sains Malaysia) ;
  • Lee, Chun Wei (Institute for Research in Molecular Medicine, Universiti Sains Malaysia) ;
  • Saadatnia, Geita (Institute for Research in Molecular Medicine, Universiti Sains Malaysia) ;
  • Velusamy, Nagarajan (Hospital Seberang Jaya) ;
  • Osman, Sabariah (Institute for Research in Molecular Medicine, Universiti Sains Malaysia) ;
  • Mohamad, Ahmad Munir (Institute for Research in Molecular Medicine, Universiti Sains Malaysia) ;
  • Noordin, Rahmah (Institute for Research in Molecular Medicine, Universiti Sains Malaysia)
  • Published : 2013.03.30

Abstract

Helicobacter pylori antigen was prepared from an isolate from a patient with a duodenal ulcer. Serum samples were obtained from culture-positive H. pylori infected patients with duodenal ulcers, gastric ulcers and gastritis (n=30). As controls, three kinds of sera without detectable H. pylori IgG antibodies were used: 30 from healthy individuals without history of gastric disorders, 30 from patients who were seen in the endoscopy clinic but were H. pylori culture negative and 30 from people with other diseases. OFF-GEL electrophoresis, SDS-PAGE and Western blots of individual serum samples were used to identify protein bands with good sensitivity and specificity when probed with the above sera and HRP-conjugated anti-human IgG. Four H. pylori protein bands showed good (${\geq}$ 70%) sensitivity and high specificity (98-100%) towards anti-Helicobacter IgG antibody in culture-positive patients sera and control sera, respectively. The identities of the antigenic proteins were elucidated by mass spectrometry. The relative molecular weights and the identities of the proteins, based on MALDI TOF/TOF, were as follows: CagI (25 kDa), urease G accessory protein (25 kDa), UreB (63 kDa) and proline/pyrroline-5-carboxylate dehydrogenase (118 KDa). These identified proteins, singly and/or in combinations, may be useful for diagnosis of H. pylori infection in patients.

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