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Benzochloroporphyrin Derivative Induced Cytotoxicity and Inhibition of Tumor Recurrence During Photodynamic Therapy for Osteosarcoma

  • Gong, Hai-Yang (Musculoskeletal Oncology Center, Shanghai Tenth People's Hospital, Tongji University, School of Medicine) ;
  • Sun, Meng-Xiong (Musculoskeletal Oncology Center, Shanghai Tenth People's Hospital, Tongji University, School of Medicine) ;
  • Hu, Shuo (Musculoskeletal Oncology Center, Shanghai Tenth People's Hospital, Tongji University, School of Medicine) ;
  • Tao, Ying-Ying (Department of Pharmacology, Jinling Hospital) ;
  • Gao, Bo (Musculoskeletal Oncology Center, Shanghai Tenth People's Hospital, Tongji University, School of Medicine) ;
  • Li, Guo-Dong (Musculoskeletal Oncology Center, Shanghai Tenth People's Hospital, Tongji University, School of Medicine) ;
  • Cai, Zheng-Dong (Musculoskeletal Oncology Center, Shanghai Tenth People's Hospital, Tongji University, School of Medicine) ;
  • Yao, Jian-Zhong (Department of Medicinal Chemistry, School of Pharmacy, Second Military Medical University)
  • Published : 2013.05.30

Abstract

Photodynamic therapy (PDT) is a promising cancer treatment modality that uses dye-sensitized photooxidation of biologic matter in target tissue. This study explored effects of the photosensitizer BCPD-17 during PDT for osteosarcoma. LM-8 osteosarcoma cells were treated with BCPD-17 and cell viability after laser irradiation was assessed in vitro with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of BCPD-17 during PDT recurrence were then examined on tumor-bearing mice in vivo. BCPD-17 had dosedependent cytotoxic effects on LM-8 osteosarcoma cells after laser irradiation which also had energy-dependent effects on the cells. The rate of local recurrence was reduced when marginal resection of mice tumors was followed by BCPD-17-mediated PDT. Our results indicated BCPD-17-mediated PDT in combination with marginal resection of tumors is a potentially new effective treatment for osteosarcoma.

Keywords

Inhibiting rate;photochemotherapy;apoptosis/drug effects;photosensitizing agents

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