- Volume 14 Issue 5
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In Vitro Antitumor Properties of an Isolate from Leaves of Cassia alata L
- Olarte, Elizabeth Iglesias (Institute of Biology, College of Science, University of the Philippines) ;
- Herrera, Annabelle Aliga (Institute of Biology, College of Science, University of the Philippines) ;
- Villasenor, Irene Manese (Institute of Chemistry, College of Science, University of the Philippines) ;
- Jacinto, Sonia Donaldo (Institute of Biology, College of Science, University of the Philippines)
- Published : 2013.05.30
Leaf extracts of Cassia alata L (akapulko), traditionally used for treatment of a variety of diseases, were evaluated for their potential antitumor properties in vitro. MTT assays were used to examine the cytotoxic effects of crude extracts on five human cancer cell lines, namely MCF-7, derived from a breast carcinoma, SK-BR-3, another breast carcinoma, T24 a bladder carcinoma, Col 2, a colorectal carcinoma, and A549, a nonsmall cell lung adenocarcinoma. Hexane extracts showed remarkable cytotoxicity against MCF-7, T24, and Col 2 in a dose-dependent manner. This observation was confirmed by morphological investigation using light microscopy. Further bioassay-directed fractionation of the cytotoxic extract led to the isolation of a TLC-pure isolate labeled as f6l. Isolate f6l was further evaluated using MTT assay and morphological and biochemical investigations, which likewise showed selectivity to MCF-7, T24, and Col 2 cells with
Cassia alata L;in vitro antitumor property;MTT formazan assay;TUNEL;caspase assay
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