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Upregulation of MicroRNA 181c Expression in Gastric Cancer Tissues and Plasma

  • Cui, Mei-Hua (Department of Gastroenterology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University) ;
  • Hou, Xiao-Lin (Department of Gastroenterology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University) ;
  • Lei, Xiao-Yan (Department of Gastroenterology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University) ;
  • Mu, Fang-Hong (Department of Gastroenterology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University) ;
  • Yang, Gui-Bin (Department of Gastroenterology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University) ;
  • Yue, Lin (Department of Gastroenterology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University) ;
  • Fu, Yi (Department of Gastroenterology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University) ;
  • Yi, Guo-Xing (Department of Gastroenterology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University)
  • Published : 2013.05.30

Abstract

Objective: To test the microRNA-181c (miR-181c) expression in tissues and plasma of gastric cancer (GC) cases, analyze any correlations, and explore the possibility of miR-181c as a potential molecular marker for GC diagnosis. Materials and Methods: Relative miR-181c expression levels in cancers and plasma from 30 GC patients was tested using reverse transcription-real-time fluorescent quantitation PCR and compared to that in samples from 30 gastric ulcer and 30 chronic gastritis patients. Results: The miR-181c expression level in the GC tissues was significantly higher than that in the gastric ulcer and chronic gastritis tissues (P = 0.000), as was the miR-181c expression level in the GC plasma (P = 0.000). We determined that miR-181c expression in GC plasma was positively correlated to its expression in the GC tissues (P = 0.000). Conclusions: The expression of miR-181c is upregulated in GC tissues and plasma, and the miR-181c expression level in GC plasma is positively correlated to that in the corresponding cancer tissues. Plasma miR-181c is possibly a new serological marker for GC diagnosis.

Keywords

MicroRNA;181c (miR-181c);gastric cancer;plasma;cancer tissue

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