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Synergistic Anticancer Activity of 5-Aminolevulinic Acid Photodynamic Therapy in Combination with Low-dose Cisplatin on Hela Cells

  • Wei, Xiao-Qiang (Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University) ;
  • Ma, Hui-Qing (Department of Gynecology and Obstetrics, The Second Affiliated Hospital of Qingdao University Medical College) ;
  • Liu, Ai-Hong (Department of Gynecology and Obstetrics, Qingdao Haici Medical Group) ;
  • Zhang, You-Zhong (Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University)
  • Published : 2013.05.30

Abstract

Objective: Photodynamic therapy (PDT ) is a promising modality for the treatment of various tumors. In order to assist in optimizing treatment, we applied 5-ALA/PDT in combination with low-dose cisplatin to evaluate cytotoxicity in Hela cells. Methods: Antiproliferative effects of 5-ALA/PDT and cisplatin, alone and in combination, were assessed using MTT assay. To examine levels of apoptosis, Hela cells treated with 5-ALA/PDT, and combination treatment were assessed with Annexin-V/PI by flow cytometry. To investigate the molecular mechanisms underlying alterations in cell proliferation and apoptosis, Western blot analysis was conducted to determine the expression of p53, p21, Bax and Bcl-2 proteins. Results: MTT assays indicated that combination treatment obviously decreased the viability of Hela cells compared to individual drug treatment. In addition, it was confirmed that exposure of Hela cells to 5-ALA/PDT in combination with low-dose cisplatin resulted in more apoptosis in vitro. Synergistic anticancer activity was related to upregulation p53 expression and alteration in expression of p21, Bcl-2 and Bax. Conclusion: Our findings suggest that administration of 5-ALA/PDT in combination with the low-dose cisplatin may be an effective and feasible therapy for cervical cancer.

Keywords

5-ALA/PDT;low-dose cisplatin;combination treatment;synergestic anticancer activity

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