Inhibitory Effects of Syk Transfection on Lung Cancer Cell Invasion

  • Peng, Chuan-Liang (Department of Thoracic, The Second Hospital of Shandong University) ;
  • Zhang, Ying (Department of Respiratory Medicine, The Second Hospital of Shandong University) ;
  • Sun, Qi-Feng (Department of Thoracic, The Second Hospital of Shandong University) ;
  • Zhao, Yun-Peng (Department of Thoracic, The Second Hospital of Shandong University) ;
  • Hao, Ying-Tao (Department of Thoracic, The Second Hospital of Shandong University) ;
  • Zhao, Xiao-Gang (Department of Thoracic, The Second Hospital of Shandong University) ;
  • Cong, Bo (Department of Thoracic, The Second Hospital of Shandong University)
  • Published : 2013.05.30


Objective: Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro. Methods: A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined. Results: The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05). Conclusions: A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.


  1. Hoellenriegel J, Coffey GP, Sinha U, et al (2012). Selective, novel spleen tyrosine kinase (Syk) inhibitors suppress chronic lymphocytic leukemia B-cell activation and migration. Leukemia, 26, 1576-83.
  2. Layton T, Stalens C, Gunderson F, et al (2009). Syk tyrosine kinase acts as a pancreatic adenocarcinoma tumor suppressor by regulating cellular growth and invasion. Am J Pathol, 175, 2625-36.
  3. Li X, Martin F, Oliver AM, Kearney JF, Birmingham AL (2001). Antigen receptor proximal signaling in splenic B-2 cell subsets. J Immunol, 166, 3122- 9.
  4. Nakashima H, Natsugoe S, Ishigami S, et al (2006). Clinical significance of nuclear expression of spleen tyrosine kinase (Syk) in gastric cancer. Cancer Lett, 8, 89-94.
  5. Peng C, Sun Q, Hao Y, et al (2013). Syk is low-expressed in nonsmall-cell lung cancer and inversely correlates with patient's survival. Acta Biochim Biophys sin (Shanghai), 45, 149-51.
  6. Sung YM, Xu X, Sun J, et al (2009). Tumor suppressor function of Syk in human MCF10A in vitro and normal mouse mammary epithelium in vivo. PloS One, 4, 7445.
  7. Toyama T, Iwase H, Yamashita H, et al (2003). Reduced expression of the Syk gene is correlated with poor prognosis in human breast cancer. Cancer Lett, 189, 97-102.
  8. Ulanova M, Puttagunta L, Marcet-Palacios M, et a1 (2005). Syk tyrosine kinase participates in betal-integrin signaling and inflammatory responses in airway epithelial cells. Am J Physiol Lung Cell Mol Physiol, 288, 497-507.
  9. Yang Z, Huo L, Chen H, et al (2013). Hypermethylation and prognostic implication of Syk gene in human colorectal cancer. Med Onclo, 30, 586.
  10. Coopman PJ, Mueller SC (2006). The Syk tyrosine kinase: a new negative regulator in tumor growth and progression. Cancer Lett, 241, 159-73.
  11. Hoeler C, Thallinger C, Pratscher B, et a1 (2005). The nonreceptor-associated tyrosine kinase Syk is a regulator of metastatic behavior in human nlelanonla cells. J Invest Dermatol, 124, 1293-9.

Cited by

  1. Alternative splicing of spleen tyrosine kinase differentially regulates colorectal cancer progression vol.12, pp.3, 2016,