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The KIF1B (rs17401966) Single Nucleotide Polymorphism is not Associated with the Development of HBV-related Hepatocellular Carcinoma in Thai Patients

  • Sopipong, Watanyoo (Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University) ;
  • Tangkijvanich, Pisit (Research Unit of Hepatitis and Liver Cancer, Faculty of Medicine, Chulalongkorn University) ;
  • Payungporn, Sunchai (Department of Biochemistry, Faculty of Medicine, Chulalongkorn University) ;
  • Posuwan, Nawarat (Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University) ;
  • Poovorawan, Yong (Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University)
  • Published : 2013.05.30

Abstract

Hepatitis B virus (HBV) infection can become chronic and if left untreated can progress to hepatocellular carcinoma (HCC).Thailand is endemic for HBV and HCC is one of the top five cancers, causing deaths among Thai HBV-infected males. A single nucleotide polymorphism (SNP) at the KIF1B gene locus, rs17401966, has been shown to be strongly associated with the development of HBV-related HCC. However, there are no Thai data on genotypic distribution and allele frequencies of rs17401966. Thai HBV patients seropositive for HBsAg (n=398) were therefore divided into two groups: a case group (chronic HBV with HCC; n=202) and a control group (HBV carriers without HCC; n=196). rs17401966 was amplified by polymerase chain reaction (PCR) and analyzed by direct nucleotide sequencing. The genotypic distribution of rs174019660 for homozygous major genotype (AA), heterozygous minor genotype (AG) and homozygous minor genotype (GG) in the case group was 49.5% (n=100), 40.1% (n=81) and 10.4% (n=21), respectively, and in controls was 49.5% (n=97), 42.3% (n=83) and 8.2% (n=16). Binary logistic regression showed that rs17401966 was not statistically associated with the risk of HCC development in Thai chronic HBV patients (p-value=0.998, OR=1.00 and 95% CI=0.68-1.48). In conclusion, the KIF1B gene SNP (rs174019660) investigated in this study showed no significant association with HBV-related HCC in Thai patients infected with HBV, indicating that there must be other mechanisms or pathways involved in the development of HCC.

Keywords

KIF1B;rs17401966;chronic hepatitis B infection (HBV);HCC;Thailand

References

  1. Abdo AA, Al-Jarallah BM, Sanai FM et al (2006). Hepatitis B genotypes: relation to clinical outcome in patients with chronic hepatitis B in Saudi Arabia. World J Gastroenterol, 12, 7019-24
  2. Al-Qahtani A, Al-Anazi M, Viswan NA, et al (2012). Role of single nucleotide polymorphisms of KIF1B gene in HBVassociated viral hepatitis. PloS one, 7, 45128. https://doi.org/10.1371/journal.pone.0045128
  3. Bagchi A, Mills AA (2008). The quest for the 1p36 tumor suppressor. Cancer Res, 68, 2551-56. https://doi.org/10.1158/0008-5472.CAN-07-2095
  4. El-Serag HB, Rudolph KL (2007). Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology, 132, 2557-76. https://doi.org/10.1053/j.gastro.2007.04.061
  5. Fattovich G, Stroffolini T, Zagni I, et al (2004). Hepatocellular carcinoma in cirrhosis: incidence and risk factors. Gastroenterology, 127, 35-50.
  6. Groopman JD, Johnson D, Kensler TW (2005). Aflatoxin and hepatitis b virus biomarkers: a paradigm for complex environmental exposures and cancer risk. Cancer Biomarkers, 1, 5-14.
  7. Kim H, Jee YM, Song BC, et al (2007). Molecular epidemiology of hepatitis B virus (HBV) genotypes and serotypes in patients with chronic HBV infection in Korea. Intervirology, 50, 52-7. https://doi.org/10.1159/000096313
  8. Lee AT, Lee CG (2007). Oncogenesis and transforming viruses: the hepatitis B virus and hepatocellular carcinoma?the etiopathogenic link. Front Biosci, 12, 234-45. https://doi.org/10.2741/2061
  9. Matsushita M, Tanaka S, Nakamura N, et al (2004). A novel kinesin-like protein, KIF1Bbeta3 is involved in the movement of lysosomes to the cell periphery in nonneuronal cells. Traffic, 5, 140-51. https://doi.org/10.1111/j.1600-0854.2003.00165.x
  10. Matsuura K, Tanaka Y, Hige S, et al (2009). Distribution of hepatitis B virus genotypes among patients with chronic infection in Japan shifting toward an increase of genotype A. J Clin Microbiol, 47, 1476-83. https://doi.org/10.1128/JCM.02081-08
  11. Mok H, Shin H, Kim S, et al (2002). Association of the kinesin superfamily motor protein KIF1Balpha with postsynaptic density-95 (PSD-95), synapse-associated protein-97, and synaptic scaffolding molecule PSD-95/discs large/zonaoccludens-1 proteins. J Neurosci, 22, 5253-8.
  12. Montesano R, Hainaut P, Wild CP (1997). Hepatocellular carcinoma: from gene to public health. J Natl Cancer Inst, 89, 1844-51. https://doi.org/10.1093/jnci/89.24.1844
  13. Morgan TR, Mandayam S, Jamal MM (2004). Alcohol and hepatocellular Carcinoma. Gastroenterology, 127, 87-96. https://doi.org/10.1053/j.gastro.2004.09.020
  14. Munirajan AK, Ando K, Mukai A (2008). KIF1Bbeta functions as a Haploinsufficient tumor suppressor gene mapped to chromosome 1p36.2 by inducing apoptotic cell death. J Biol Chem, 283, 24426-34. https://doi.org/10.1074/jbc.M802316200
  15. Nangaku M, Sato-Yoshitake R, Okada Y, et al (1994). KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria. Cell, 79, 1209-20. https://doi.org/10.1016/0092-8674(94)90012-4
  16. Persson EC, Schwartz LM, Park Y, et al (2013). Alcohol consumption, folate intake, hepatocellular carcinoma, and liver disease mortality. Cancer Epidemiol Biomarkers Prev, 22, 415-21. https://doi.org/10.1158/1055-9965.EPI-12-1169
  17. Sawai H, Nishida N, Mbarek H, et al (2012). No association for Chinese HBV-related Hepatocellular carcinoma susceptibility SNP in order East Asian populations. BMC Med Genet, 13, 47.
  18. Schlisio S, Kenchappa RS, Vredeveld LC, et al (2008). The kinesin KIF1Bbeta acts downstream from EglN3 to induce apoptosis and is a potential 1p36 tumor suppressor. Genes Dev, 22, 884-93. https://doi.org/10.1101/gad.1648608
  19. Srivatanakul P, Sriplung H, Deerasamee S (2004). Epidemiology of liver cancer: an overview. Asian Pac J Cancer Prev, 5, 118-25.
  20. Tangkijvanich P, Hirsch P, Theamboonlers A, et al (1999). Association of hepatitis viruses with hepatocellular carcinoma in Thailand. J Gastroenterol, 34, 227-33. https://doi.org/10.1007/s005350050248
  21. Tangkijvanich P, Mahachai V, Komolmit P, et al (2005). Hepatitis B virus genotypes and Hepatocellular carcinoma in Thailand. World J Gastroenterol, 11, 2238-43. https://doi.org/10.3748/wjg.v11.i15.2238
  22. Tangkijvanich P, Theamboonlers A, Sriponthong M, et al (2003). SEN virus infection and the risk of hepatocellular carcinoma: a case-control study. Am J Gastroenterol, 98, 2500-4. https://doi.org/10.1111/j.1572-0241.2003.07689.x
  23. Wong CM, Ng IO (2008). Molecular pathogenesis of hepatocellular carcinoma. Liver Int, 28, 160-74.
  24. Yang HI, Yeh SH, Chen PJ, et al (2008). Associations between hepatitis B virus genotype and mutants and the risk of hepatocellular carcinoma. J Natl Cancer Inst, 100, 1134-43. https://doi.org/10.1093/jnci/djn243
  25. Yang HW, Chen YZ, Takita J, et al (2001). Genomic structure and mutational analysis of the human KIF1B gene which is homozygously deleted in neuroblastoma at chromosome 1p36.2. Oncogene, 20, 5075-83. https://doi.org/10.1038/sj.onc.1204456
  26. Zhang H, Zhai Y, Hu Z, et al (2010). Genome-wide association study identifies1p36.2 as a new susceptibility locus for hepatocellular carcinoma in Chronic hepatitis B virus carriers. Nat Genet, 42, 755-8. https://doi.org/10.1038/ng.638
  27. Zhao C, Takita J, Tanaka Y, et al (2001). Charcot-Marie-Tooth disease type 2A caused by mutation in a microtubule motor KIF1Bbeta. Cell, 105, 587-97. https://doi.org/10.1016/S0092-8674(01)00363-4
  28. Zhu CT, Dong CL (2009). Characteristics of hepatitis B virus genotypes in China. Hepatobiliary Pancreat Dis Int, 8, 397-401.

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