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Ellagic Acid Inhibits Migration and Invasion by Prostate Cancer Cell Lines

  • Pitchakarn, Pornsiri (Department of Biochemistry, Faculty of Medicine, Chiang Mai University) ;
  • Chewonarin, Teera (Department of Biochemistry, Faculty of Medicine, Chiang Mai University) ;
  • Ogawa, Kumiko (Department of Experimental Pathology and Tumor Biology, Nagoya City University, Graduate School of Medical Sciences) ;
  • Suzuki, Shugo (Department of Experimental Pathology and Tumor Biology, Nagoya City University, Graduate School of Medical Sciences) ;
  • Asamoto, Makoto (Department of Experimental Pathology and Tumor Biology, Nagoya City University, Graduate School of Medical Sciences) ;
  • Takahashi, Satoru (Department of Experimental Pathology and Tumor Biology, Nagoya City University, Graduate School of Medical Sciences) ;
  • Shirai, Tomoyuki (Department of Experimental Pathology and Tumor Biology, Nagoya City University, Graduate School of Medical Sciences) ;
  • Limtrakul, Pornngarm (Department of Biochemistry, Faculty of Medicine, Chiang Mai University)
  • Published : 2013.05.30

Abstract

Polyphenolic compounds from pomegranate fruit extracts (PFEs) have been reported to possess antiproliferative, pro-apoptotic, anti-inflammatory and anti-invasion effects in prostate and other cancers. However, the mechanisms responsible for the inhibition of cancer invasion remain to be clarified. In the present study, we investigated anti-invasive effects of ellagic acid (EA) in androgen-independent human (PC-3) and rat (PLS10) prostate cancer cell lines in vitro. The results indicated that non-toxic concentrations of EA significantly inhibited the motility and invasion of cells examined in migration and invasion assays. The EA treatment slightly decreased secretion of matrix metalloproteinase (MMP)-2 but not MMP-9 from both cell lines. We further found that EA significantly reduced proteolytic activity of collagenase/gelatinase secreted from the PLS-10 cell line. Collagenase IV activity was also concentration-dependently inhibited by EA. These results demonstrated that EA has an ability to inhibit invasive potential of prostate cancer cells through action on protease activity.

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