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Associations of IL-10 Gene Polymorphisms with Acute Myeloid Leukemia in Hunan, China

  • Yao, Chen-Jiao (Department of Hematology, The Third Xiangya Hospital of Central South University) ;
  • Du, Wei (Department of Hematology, The Third Xiangya Hospital of Central South University) ;
  • Chen, Hai-Bing (Department of Hematology, The Third Xiangya Hospital of Central South University) ;
  • Xiao, Sheng (Department of Hematology, The Third Xiangya Hospital of Central South University) ;
  • Wang, Cheng-Hong (Department of Hematology, The Third Xiangya Hospital of Central South University) ;
  • Fan, Zi-Li (Department of Hematology, The Third Xiangya Hospital of Central South University)
  • 발행 : 2013.04.30

초록

We investigated the possible association of interleukin-10 (IL-10) single nucleotide polymorphisms (SNPs) and susceptibility to acute myeloid leukemia (AML) in 115 patients and 137 healthy controls. Genetic analysis of IL-10 SNPs at -819 and -592 was carried out with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach. The IL-10 mRNA expression of AML patients and controls with different genotype was detected by real-time quantitative polymerase chain reaction (RT-PCR). Genetic analysis of IL-10 revealed that the -819AA genotype frequencies and the -819A allele frequencies in the AML group were higher than in the controls (59.1% vs 40.9%; 75.6% vs 63.9%, respectively); there were remarkable differences in -819T/C and -592A/C gene distribution (P<0.05) and the TA haploid frequencies were higher in the AML group (75.6% vs 63.9%, P<0.05). IL-10 mRNA expression in incipient AML patients was obvious higher than the non-tumor group and the remission group ($7.78{\times}10^{-3}$ vs $2.43{\times}10^{-3}$, $3.64{\times}10^{-3}$, P<0.05).The study suggested that the haploid TA and genotype TA/TA may be associated with AML in Han people in Hunan province.The IL-10 SNPs at -819 and -592 sites were associated with AML and may affect IL-10 mRNA expression in AML patients.

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