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Root Colonization and Quorum Sensing of the Antagonistic Bacterium Pseudomonas fluorescens 2112 involved in the Red-pepper Rhizosphere

생물방제균 Pseudomonas fluorescens 2112의 고추 근권정착능과 Quorum-sensing 기능

  • Jung, Byung-Kwon (Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University) ;
  • Kim, Yo-Hwan (Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University) ;
  • Kim, Sang-Dal (Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University)
  • 정병권 (영남대학교 미생물생명공학과) ;
  • 김요환 (영남대학교 미생물생명공학과) ;
  • 김상달 (영남대학교 미생물생명공학과)
  • Received : 2012.10.17
  • Accepted : 2012.11.15
  • Published : 2013.03.28

Abstract

Biofilm formation of multifunctional plant growth promoting rhizobacterium (PGPR), Pseudomonas fluorescens 2112 is necessary for P. fluorescens 2112 to have a positive impact on the rhizosphere of red-pepper. This study investigated whether signal molecules of the quorum sensing AHLs are produced in order to confirm biofilm formative ability. Through the use of Petri dish bioassays a blue circle formed evidence of AHLs. It was confirmed that P. fluorescens 2112 produced six-carbon-chain-long AHLs by TLC bioassay. The bacterial density of P. fluorescens 2112 on the top and bottom of pepper plant roots was estimated as $3{\times}10^5$ and $8{\times}10^3$ CFU/g root, respectively. P. fluorescens 2112 exist more with high-density of $3.5{\times}10^6$ CFU/g soil at a depth of 1 cm but at a low-density of $1.1{\times}10$ CFU/g soil at a depth of 5 cm, from the surface of rhizosphere soil. In addition, biofilm formation of P. fluorescens 2112 on the epidermises and the tips of the red-pepper roots were confirmed visually by SEM. Thus, the production of AHLs by P. fluorescens 2112 brings about quorum sensing signaling and the formation of biofilm on the roots which has a positive effect on economically important crops such as red-pepper by additionally producing a variety of antifungal substances and auxin.

Keywords

Quorum sensing;biofilm;PGPR;biological control;P. fluorescens 2112

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