- Volume 41 Issue 1
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Antibiotic Resistance and Bacterial Biofilm Formation by Staphylococcus aureus Strains Isolated from Various Foods
식품에서 분리한 황색포도상구균의 항생제 내성 특징 및 균막 형성
- Lee, Joo-Young (Food Analysis Center, Korea Food Research Institute) ;
- Wang, Hae-Jin (Food Analysis Center, Korea Food Research Institute) ;
- Shin, Dong-Bin (Food Analysis Center, Korea Food Research Institute) ;
- Cho, Yong-Sun (Food Analysis Center, Korea Food Research Institute)
- Received : 2012.08.30
- Accepted : 2012.11.13
- Published : 2013.03.28
Staphylococcus aureus is a major human pathogen that produces a wide array of toxins, leading to a number of adverse symptoms. We examined 275 strains of Staphylococcus aureus isolated from various foods between 2006 and 2008 for antimicrobial susceptibility. At least 259 (94.2%) of the tested strains showed antibiotic resistant properties, and 106 (40.7%) of them showed multiple antibiotic resistance. Eleven of the tested strains were resistant to oxacillin and mec A-positive. Moreover, oxacillin-resistant strains were significantly more likely to be multi-drug resistant (p < 0.01). Of the 275 isolates tested, 24.4% were noted as being positive for slime production and 30.5% were positive for biofilm assay. Antibiotic resistance was not associated with a significantly higher prevalence of biofilm formation. Twenty strains were classified using the DiversiLab system. Most of the strains could be classified into 2 clusters and 4 unique types. All 10 mec A-positive strains (cluster I) were grouped together into the same sub-cluster. Cluster II (6 strains) was not found to be resistant to oxacillin in this study. Although the prevalence of methicillin-resistant S. aureus in food is currently low, the risk of its transmission through the food chain cannot be disregarded.
Staphylococcus aureus;antibiotic resistance;biofilm;MRSA
- Chery, K. S., J. I. Pounder, S. R. Page, B. J. Schaecher, and G. L. Woods. 2005. Clinical Evaluation of the DiversiLab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains. J. Clin. Microbiol. 43: 1187-1192. https://doi.org/10.1128/JCM.43.3.1187-1192.2005
- Chmielewski, R. A. N. and J. F. Frank. 2003. Biofilm formation and control in food processing facilities. Compr. Rev. Food Sci. Food Saf. 2: 22-32. https://doi.org/10.1111/j.1541-4337.2003.tb00012.x
- Cho, K. J., S. Jin, J. H. Cui, T. R. Yoon, and P. Y. Ryu. 2008. Effects of biofilm formation on the antimicrobial susceptibility of Staphylococcus aureus. J. Bacteriol. Virol. 38: 197-205. https://doi.org/10.4167/jbv.2008.38.4.197
- Freeman, D. J., F. R. Falkiner, and C. T. Keane. 1989. New method for detecting slime production by coagulase negative staphylococci. J. Clin. Pathol. 42: 872-874. https://doi.org/10.1136/jcp.42.8.872
- Hwang, I. G., H. S. Kwak, and S. H. Yoon. 2010. Methicillinresistant Staphylococcus aureus (MRSA) as a foodborne biological hazard. J. Food Hyg. Safety 50: 26-36.
- Johannes, K. M., A. H. Matthiab, R. Hologer, and M. Dietrich. 2002. Evaluation of different detection methods of biofilm formation in Staphylococcus aureus. Med. Microbiol. Immunol. 191: 101-106. https://doi.org/10.1007/s00430-002-0124-3
- KFDA. 2009. Repot of 2009 Estabililshment of a risk profile for methicillin-resistant Staphylococcus aureus (MRSA). Korea Food and Drug Administration, Cheongwon, Korea.
- KFDA. 2010. Statistical yearbook. Food - borne disease outbreaks, Korea Food and Drug Administration, Cheongwon, Korea.
- Kim, J. S., H. S. Kim, W. Song, H. C. Cho, K. M Lee, and E. C. Kim. 2004. Antimicrobial ressistat profiles of Staphylococcus aureus isolated in 13 Korea hospitals. Korean J. Lab. Med. 24: 223-229.
- Kumar, C. G. and S. K. Anand. 1998. Significance of microbial biofilms in food industry. Int. J. Food Microbiol. 42: 9-27. https://doi.org/10.1016/S0168-1605(98)00060-9
- Kwon, A.S., G. C. Park,, S. Y. Ryu, D. H. Lim, D. Y. Lim, C. H. Choi, Y. K. Park, and Y. Lim. 2008. Higher biofilm formation in multidrug-resistant clinical isolates of Staphylococcus aureus. Int. J. Antimicrob. Agents 32: 68-72. https://doi.org/10.1016/j.ijantimicag.2008.02.009
- Kwon, Y. I., T. W. Kim, H. Y. Kim, Y. H. Chang, H. S. Kwak, and G. J. Woo. 2007. Monitoring of methicillin resistant Staphylococcus aureus from medical environment in Korea. Korean J. Microbiol. Biotechnol. 35: 158-162.
- Lee, W. C., T. Sakai, M. J. Lee, M. Hamakawa, S. M. Lee, and I. M. Lee. 1996. An epidemiological study of food poisoning in Korea and Japan. Int. J. Food Microbiol. 29: 141-148 . https://doi.org/10.1016/0168-1605(95)00075-5
- Lim, S. K., H. M. Nam, H. J. Park, H. S. Lee, M. J. Choi, S. C. Jung, J. Y. Lee, Y. C. Kim, S. W. Song, and S. H. Wee. 2010. Prevalence and characterization of methicillin -resistant Staphylococcus aureus in raw meat in Korea. J. Microbiol. Biotechnol. 20: 775-778.
- Marco, L., B. Cinzia, G. B. Maria, F. Maria, Z. Jessica, and F. Roberta. 2002. Evaluation of the VITEK 2 system for identification and antimicrobial susceptibility testing of medically relevant gram-positive cocci. J. Clin. Microbiol. 40: 1681-1686. https://doi.org/10.1128/JCM.40.5.1681-1686.2002
- Park J. I. and N. L. Lee. 1998. Comparison of susceptibility test and meca detection for determination of methicillin resistance in Staphylococcus epidermidis. Korean J. Clin. Pathol. 18: 391-395.
- Perez-Roth, E., F. C. Martyin, J. Villar, and S. M. Alvarez. 2001. Multiplex PCR for simultaneous identification of Staphylococcus aureus and detection of methicillin and mupirocin resistance. J. Clin. Microbiol. 39: 4037-4041. https://doi.org/10.1128/JCM.39.11.4037-4041.2001
- Philip, S. S. and J. W. Costerton. 2001. Antibioric resistance of bacteria in biofilms. J. Lancet. 358: 135-138. https://doi.org/10.1016/S0140-6736(01)05321-1
- Rhee, C. H. and G. J. Woo. 2010. Emergence and characterization of foodborne methicillin-resistant Staphylococcus aureus in Korea. J. Food Prot. 73: 2285-2290.
- Ross, T. L., W. G. Merz, M. Farkosh, and K. C. Carroll. 2005. Comparison of an automated repetitive sequence-based pcr microbial typing system to pulsed-field gel electrophoresis for analysis of outbreaks of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 5642-5647. https://doi.org/10.1128/JCM.43.11.5642-5647.2005
- Shutt, C.K., J. I. Pounder, S. R. Page, B. J. Schaecher, and G. L. Woods. 2005. Clinical evaluation of the diversilab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains. J. Clin. Microbiol. 43: 1187-1192. https://doi.org/10.1128/JCM.43.3.1187-1192.2005
- Timothy, F. J., M. E. Kellum, S. S. Porter, M. Bell, and W. Schaffner. 2002. An Outbreak of community-acquired foodborne illness caused by methicillin-resistant Staphylococcus aureus. J. Emerg. Infect. Dis. 8: 82-84. https://doi.org/10.3201/eid0801.010174
- Vasudevan, P., K. M. N. Manoj, T. Annamalai, and K. S. Venkitanarayanan. 2003. Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Vet. Microbiol. 92: 179-185. https://doi.org/10.1016/S0378-1135(02)00360-7
- Wei, H. L. and C. S. Chiou. 2002. Molecular subtyping of Staphylococcus aureus from an outbreak associated with a food handler. Epidemiol. Infect. 128: 15-20.
- Witta, R., V. Kanhaib, and W. B. Leeuwena. 2009. Comparison of the DiversiLabTM system, pulsed-field gel electrophoresis and multi-locus sequence typing for the characterization of epidemic reference MRSA strains. J. Microbiol. Methods 77: 130-133. https://doi.org/10.1016/j.mimet.2009.01.009