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Isolation, Identification and Mutant Development of Butanol Tolerance Bacterium

부탄올 내성 미생물의 분리, 동정 및 변이주의 개발

  • Jung, Hyesook (Department of Food Science & Biotechnology, Kyungsung University) ;
  • Lee, Jinho (Department of Food Science & Biotechnology, Kyungsung University)
  • 정혜숙 (경성대학교 식품생명공학과) ;
  • 이진호 (경성대학교 식품생명공학과)
  • Received : 2012.10.23
  • Accepted : 2012.12.05
  • Published : 2013.03.28

Abstract

Butanol-resistant bacteria were isolated from butanol solvent. The cell growth of isolated strains declined with increasing concentrations of butanol, and isolated strain BRS02 displayed more resistance to 12.5 g/L of butanol than other isolated strains. In addition, strain BRS251, which was resistant to even higher concentrations of butanol, was developed by the mutation of BRS02 using UV. BRS251 could grow in LB medium containing up to 17.5 g/L of butanol, 32.5 g/L of propanol, or 6 g/L of pentanol, whereas the control strain Escherichia coli was found to be tolerant to 7.5 g/L of butanol, 20 g/L of propanol, or 2 g/L of pentanol. The isolated BRS02, a Gram(+) bacterium seen to have a cocci form under the microscope, grew in 6.5% NaCl. According to biochemical tests, BRS02 can metabolize and produce acid with D-galactose, D-maltose, D-mannitol, D-mannose, methyl-${\beta}$-Dglucopyranoside, D-ribose, sucrose, or D-trehalose, as carbon sources. Also, this strain showed resistance to bacitracin, vibriostatic agent O/129, and optochin, alongside positive activities for arginine dihydrolase, ${\alpha}$-glucosidase, and urease. The BRS02 strain was identified as Staphylococcus sp. by analyses of the 16S rRNA gene, phylogenetic tree, and biochemical tests.

Keywords

Butanol;tolerance;Staphylococcus;mutagenesis

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