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Isolation and Characterization of a Novel Agar Degrading Bacterium, Alteromonas macleodii subsp. GNUM08120, from Red Macroalgae

홍조류로부터 신규 한천분해미생물 Alteromonas macleodii subsp. GNUM08120의 분리 및 동정

  • Chi, Won-Jae (Department of Biological Science, Myongji University) ;
  • Lim, Ju-Hyeon (Department of Biological Science, Myongji University) ;
  • Park, Da Yeon (Department of Biological Science, Myongji University) ;
  • Kim, Mu-Chan (Department of Marine Environmental Engineering, Gyeongsang National University) ;
  • Kim, Chang-Joon (Department of Chemical and Biological Engineering, Gyeongsang National University) ;
  • Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Hong, Soon-Kwang (Department of Biological Science, Myongji University)
  • 지원재 (명지대학교 생명과학정보학부) ;
  • 임주현 (명지대학교 생명과학정보학부) ;
  • 박다연 (명지대학교 생명과학정보학부) ;
  • 김무찬 (경상대학교 생명화학공학과 및 공학연구원) ;
  • 김창준 (경상대학교 해양환경공학과 및 해양산업 연구소) ;
  • 장용근 (한국과학기술원 생명화학공학과) ;
  • 홍순광 (명지대학교 생명과학정보학부)
  • Received : 2012.08.01
  • Accepted : 2012.10.02
  • Published : 2013.03.28

Abstract

An agar-hydrolyzing marine bacterium, strain GNUM08120, was isolated from Sargassum fulvellum collected from Yeongil bay of East Sea of Korea. The isolate was Gram-negative, aerobic, motile with single polar flagellum, and grew at 1-10% NaCl, pH 5.0-8.0, and $15-37^{\circ}C$. G+C content and the predominant respiratory quinone were 46.13 mol% and Q-8, respectively. The major cellular fatty acids were Summed feature 3 (24.5%), $C_{16:0}$ (21.7%), and $C_{18:1}{\omega}7c$ (12.5%). Based on 16S rRNA gene sequence similarity and DNA-DNA hybridization analyses, strain GNUM08120 was identified as a novel subspecies of Alteromonas macleodii, designated Alteromonas macleodii subsp. GNUM08120. Production of agarase by strain GNUM08120 was likely repressed by the effect of carbon catabolite repression caused by glucose. The crude agarase prepared from 12-h culture broth of strain GNUM08120 exhibited an optimum pH and temperature for agarase activity at 7.0 and $40^{\circ}C$, respectively. The crude enzyme produced (neo)agarobiose, (neo)agarotetraose, and (neo)agarohexaose as the hydrolyzed product of agarose.

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