Evaluation of Bioethanol Productivity from Sorghum × Sudangrass Hybrid for Cellulosic Feedstocks

셀룰로오스계 원료작물로서 수수-수단그래스 교잡종의 바이오에탄올 생산량 평가

  • 차영록 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 문윤호 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 구본철 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 안종웅 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 윤영미 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 남상식 (농촌진흥청 국립식량과학원 기획조정과) ;
  • 김중곤 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 안기홍 (농촌진흥청 국립식량과학원 바이오에너지작물센터) ;
  • 박광근 (농촌진흥청 국립식량과학원 바이오에너지작물센터)
  • Received : 2012.11.09
  • Accepted : 2013.01.21
  • Published : 2013.03.31


The world demand of renewable bioenergy as an alternative transportation fuel is greatly increasing. Research for bioethanol production is currently being progressed intensively throughout the world. Therefore it will be necessary to develop bioethanol production with cellulosic materials. In this study, the yield of ethanol production was evaluated by simultaneous saccharification and fermentation (SSF) using sodium hydroxide pretreated sorghum ${\times}$ sudangrass hybrids. Composition analysis of 11 varieties of sorghum ${\times}$ sudangrass hybrids was performed for selection of excellent variety to efficiently produce bioethanol. The content of cellulose, hemicellulose, lignin and ash of these varieties were 32~39%, 19~24%, 17~22% and 6~11%, respectively. Among these varieties, 4 varieties of sorghum ${\times}$ sudangrass hybrids were selected for the evaluation of ethanol yield and those were pretreated with 1 M NaOH solution at $150^{\circ}C$ for 30 min using high temperature explosion system. After pretreatment, samples were neutralized with tap water. It contained 52~57% of cellulose. Simultaneous saccharification and fermentation (SSF) was carried out for 48 h at $33^{\circ}C$ by Saccharomyces cerevisiae CHY1011 using Green star variety. The yield of ethanol was 92.4% and the amount of ethanol production was estimated at 6206 L/ha.


  1. Hendriks A. T. W. M., and G. Zeeman. 2009. Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technology 100 : 10-18.
  2. Saha B. C., L. B. Iten, M. A. Cotta, and Y. V. Wu. 2005. Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol. Process biochemistry 40 : 3693-3700.
  3. Wang D. I .C., G. C. Avgerinos, I. Biocic, S. D. Wang, and H. Y. Fang. 1983. Ethanol from cellulosic biomass. Phil. Trans. R. Soc. Lond. B 300 : 323-333.
  4. Alizadeh H., F. Teymouri, T. O. Gilbert, and B. E. Dale. 2005. Pretreatment of Switchgrass by Ammonia Fiber Explosion (AFEX). Applied Biochemistry and Biotechnology 121-124 : 1133-1141.
  5. Yoo H. Y., S. B. Kim, H. S. Choi, and K. Kim. Optimization of Sodium Hydroxide pretreatment of Canola Agricultural Residues for Fermentable Sugar Production using Statistical Method. 2012. International Conference on Future Environment and Energy(IPCBEE) 28 : 175-179.
  6. Karini K., C. Emtiazi, and M. J. Taherzadeh. 2006. Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae. Enzyme and Microbial Technology 40 : 138-144.
  7. Lynd L. R., J. H. Cushman, R. J. Nichols, and C. E. Wyman. 1991. Fuel Ethanol from Cellulosic Biomass. Science 251 : 1318-1323.
  8. Han M. H., Y. Kim, Y. R Kim, B. W. Chungm, and G. W. Choi. 2011. Bioethanol production from optimized pretreatment of cassava stem. Korean J. Chem. Eng. 28(1) : 119-125.
  9. Lau M. W. and B. E. Dale. 2009. Cellulosic ethanol production from AFEX-treated corn stover using Saccaromyces cerevisiae 424A(LNH-ST). PNAS 106(5) : 1368-1373.
  10. Alvira P., E. Tomas-Pejo, M. Ballesteros, and M. J. Negro. 2010. Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis : A review. Bioresource Technology 101 : 4851-4861.
  11. Shao Q., S. PS. Chundawat, C. Krishnan, B. Bals, L. C. Sousa, K. D. Thelen, B. E. Dale, and V. Balan. 2010. Enzymatic digestigility and ethanol fermentability of AFEX-treated starch-rich lignocellulosics such as corn silage and whole corn plant. Biotechnology for Biofuels 3(12) : 1-10.
  12. Haan R. D., S. H. Rose, L. R. Lynd, and W. H. van Zyl. 2007. Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metabolic Engineering 9 : 87-94.
  13. Tew T. L., R.t M. Cobill, and E. P. Richard Jr. 2008. Evaluation of Sweet Sorghum and Sorghum x Sudangrass Hybrids as Feedstocks for Ethanol Production. Bioenerg. Res. 1 : 147-152.
  14. Park Y. C. and J. S. Kim. 2012. Comparison of various alkaline pretreatment methods of lignocellulosic biomass. Energy 47 : 31-35.

Cited by

  1. Enhanced saccharification of reed and rice straws by the addition of β-1,3-1,4-glucanase with broad substrate specificity and calcium ion vol.58, pp.1, 2015,
  2. Crop diversity for mixed first and second generation ethanol production 2016,