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Molecular Authentication and Phylogenetic Analysis of Plant Species for Breeae and Cirsii Herba based on DNA barcodes

DNA 바코드 분석을 통한 소계(小薊) 및 대계(大薊) 기원식물 감별과 종간 유연관계 분석

  • Moon, Byeong Cheol (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Lee, Young Mi (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Ji, Yunui (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Chun, Jin Mi (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ho Kyoung (Herbal Medicine Resources Group, Korea Institute of Oriental Medicine)
  • 문병철 (한국한의학연구원 한약자원그룹) ;
  • 이영미 (한국한의학연구원 한약자원그룹) ;
  • 지윤의 (한국한의학연구원 한약자원그룹) ;
  • 최고야 (한국한의학연구원 한약자원그룹) ;
  • 천진미 (한국한의학연구원 한약자원그룹) ;
  • 김호경 (한국한의학연구원 한약자원그룹)
  • Published : 2013.05.30

Abstract

Objectives : The origin of Breeae Herba (So-gye) and Cirsii Herba (Dae-gye) is differently prescribed in Korean and Chinese modern pharmacopoeia. Since the similar morphological characteristics and chaotic plant names, moreover, the aerial part of Carduus crispus have been used as the Cirsii Herba. To develop a reliable method for correct identification of these herbal medicines and to evaluate the genetic relationship of these closely related plant species, we analyzed sequences of DNA barcode regions. Methods : Thirty-one samples of 6 medicinal plants (B. segeta, B. setosa, C. japonicum var. maackii, C. setidens, C. chanroenicum, and C. crispus) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL) were analyzed after amplification using appropriate primers reported in previous studies. The nucleotides of species-specific authentic marker and phylogenetic relations were estimated based on the entire sequences of DNA barcodes by the analysis of ClastalW and UPGMA, respectively. Results : In comparative analysis of DNA barcode sequences, we obtained specific nucleotides to discriminate the medicinal plant of Breeae/Cirsii Herba in species level and evaluated the phylogenetic relationship of these species. Futhermore, we identified distinct marker nucleotides enough to authenticate respective species. These sequence differences at corresponding positions were avaliable genetic markers to determine the botanical origins of Breeae Herbal as well as Cirsii Herba. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by providing of definitive information that can identify each plant species and distinguish from unauthentic adulterants and substitutes.

Acknowledgement

Supported by : 한국한의학연구원

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