Memory Enhancing Effect of Codonopsis lanceolata by High Hydrostatic Pressure Process and Fermentation

초고압 발효 더덕 추출물의 인지능력 개선 효과

  • Weon, Jin Bae (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Bohyoung (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Yun, Bo-Ra (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Jiwoo (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Hyeon Yong (Department of Teaics, Seowon University) ;
  • Park, Dong-Sik (Functional food & Nutrition Division, Department of Agrofood Resources) ;
  • Chung, Hee-Chul (Newtree CO., LTD.) ;
  • Chung, Jae Youn (Newtree CO., LTD.) ;
  • Ma, Choong Je (Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University)
  • 원진배 (강원대학교 의생명소재공학전공) ;
  • 이보형 (강원대학교 의생명소재공학전공) ;
  • 윤보라 (강원대학교 의생명소재공학전공) ;
  • 이지우 (강원대학교 의생명소재공학전공) ;
  • 이현용 (서원대학교 차학과(식품공학과)) ;
  • 박동식 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 정희철 ((주)뉴트리) ;
  • 정재윤 ((주)뉴트리) ;
  • 마충제 (강원대학교 의생명소재공학전공)
  • Received : 2013.01.04
  • Accepted : 2013.03.04
  • Published : 2013.03.31

Abstract

Alzheimer's disease (AD), most common form of dementia is characterized that memory deficit and loss of cognitive function. The Codonopsis lanceolata (C.lanceolata) was treated by high hydrostatic pressure process and fermentation. This study was evaluated cognitive enhancing effect C.lanceolata extract by high hydrostatic pressure process and fermentation and compared with common C.lanceolata extract using Morris water maze and passive avoidance test. And their neuroprotective effect on glutamate induced oxidative stress in HT22 cell was investigated by MTT assay. High hydrostatic pressure process and fermented C.lanceolata extract (HFCE) and common C.lanceolata extract (CCE) (100 and 300 mg/kg) were administered to mice. Results showed HFCE enhanced cognitive function than CCE as shown by decrease in escape latency time. HFCE increased the latency time of the passive avoidance test compared to CCE. Furthermore, HFCE showed significant neuroprotective effect against glutamate cytotoxicity in HT22 cells. These results indicate that high hydrostatic pressure process and fermented more improve spatial cognitive ability of C. laanceolata.

Keywords

Acknowledgement

Supported by : 농촌진흥청

References

  1. Crapper, D. R. and DeBoni, U. (1978) Brain aging and Alzheimer's disease. Can. Psychiatr. Assoc. J. 23: 229-233.
  2. Portelius, E., Zetterberg, H., Andreasson, U., Brinkmalm, G., Andereason, N., Wallin, A., Westman-Brinkmalm, A. and Blennow, K. (2006) An Alzheimer's disease-specific betaamyloid fragment signature in cerebrospinal fulid. Neurosci. Lett. 409: 229-237.
  3. Citron, M. (2002) Alzheimer's disease: treatments in discovery and development. Nat. Neurosci. 5: 1055-1057.
  4. Bartus, R. T., Dean, R. L., Beer, D. and Lipa, A. S. (1982) The cholinergic hypothesis of geriatric memory dysfunction. Science 217: 408-417. https://doi.org/10.1126/science.7046051
  5. Dawson, G. R. and Iversen, S. D. (1993) The effects of novel cholinesterase inhibitors and selective muscarinic receptor agonists in tests of reference and working memory, Behav. Brain Res. 57: 143-153. https://doi.org/10.1016/0166-4328(93)90130-I
  6. Dastmalchi, K., Damien, D. H. J., Vuorela, H. and Hiltunen, R. (2007) Plants as potential sources of drug development against Alzheimer's disease. Int. J. Biomed. Pharmaceut. Sci. 1: 83-104.
  7. Ushijima, M., Komoto, N., Sugizono, Y., Mizuno, I., Sumihiro, M., Ichikawa, M., Hayama, M., Kawahara, N., Nakane, T., Shirota, O., Sekita, S. and Kuroyanagi, M. (2008) Triterpene glycosides from the roots of Codonopsis lanceolata. Chem. Pharm. Bull. 56: 308-314. https://doi.org/10.1248/cpb.56.308
  8. Li, J. P., Liang, Z. M. and Yuan, Z. (2007) Triterpenoid saponins and anti-inflammatory activity of Codonopsis lanceolata. Pharmazie. 62: 463-466.
  9. Wang, L., Xu, M. L., Hu, J. H., Rasmussen, S. K. and Wang, M. H. (2011) Codonopsis lanceolata extract induces G0/G1 arrest and apoptosis in human colon tumor HT-29 cells-- involvement of ROS generation and polyamine depletion. Food Chem. Toxicol. 49: 149-154. https://doi.org/10.1016/j.fct.2010.10.010
  10. Ryu, H.- S. (2009) Effect of Codonopsis lanceolatae Extracts on mouse IL-2, IFN-, IL-10 cytokine production by peritoneal macrophage and the ratio of IFN-, IL-10 cytokine. Korean J. Food Nutr. 22: 69-74.
  11. Han, C., Li, L., Piao, K., Shen, Y. and Piao, Y. (1999) Experimental study on anti-oxygen and promoting intelligence development of Codonopsis lanceolata in old mice. Zhong .Yao. Cai. 22:136-138.
  12. Choi, W.- Y., Lee, C.- G., Seo, Y.- C., Song, C.- H., Lim, H.- W. and Lee, H.- Y. (2012) Effect of high pressure and steaming extraction processes on Ginsenosides Rg3 and Rh2 contents of cultured-root in wild Ginseng (Panax ginseng C. A. Meyer). Korean J. Med. Crop Sci. 20: 270-276. https://doi.org/10.7783/KJMCS.2012.20.4.270
  13. Jeong, M. H., Choi, W. Y., Seo, Y. C., Kang, H. Y. Choi, G. P. and Lee, H. Y. (2010) Anticancer activity of Acer mono Wood extracted by ultra high pressure extraction process. Korean J. Med. Crop Sci. 18: 157-167.
  14. Park, M. R., Yoo, C., Chang, Y. N. and Ahn, B. Y. (2012) Change of total polyphenol content of fermented Gastrodia elata Blume and radical scavenging. Korean J. plant Res. 25: 379-386. https://doi.org/10.7732/kjpr.2012.25.4.379
  15. Yang, H. J., Weon, J. B., Lee, B. and Ma, C. J. (2011) The alteration of components in the fermented Hwangryunhaedok- tang and its neuroprotective activity. Pharmacogn. Mag. 7:207-212. https://doi.org/10.4103/0973-1296.84234
  16. Morris, R. (1984) Developments of water-maze procedure for studying spatial learning in rats. J. Neurosci. Methods 11: 47-60 https://doi.org/10.1016/0165-0270(84)90007-4
  17. Ebert, U. and Kirch, W. (1998) Scopolamine model of dementia: electroencephalogram findings and cognitive performance. Eur. J. Clin. Invest. 28: 944-949. https://doi.org/10.1046/j.1365-2362.1998.00393.x
  18. Blokland, A. (1995) Acetylcholine: a neurotransmitter for learning and memory? Brain Res. Rev. 21: 285-300. https://doi.org/10.1016/0165-0173(95)00016-X
  19. Collerton, D. (1986) Cholinergic function and intellectual decline in Alzheimer's disease. Neurosci. 19: 1-28. https://doi.org/10.1016/0306-4522(86)90002-3
  20. Coyle, J. T. (1993) Oxidative stress, glutamate, and neurodegenerative disorders. Science 262: 689-695. https://doi.org/10.1126/science.7901908
  21. Muphy, T. H., Miyamoto, M., Sastre, A., Schnaar, R. and Coyle, J. T. (1989) Glutamate toxicity in neuronal cell line involves inhibition of cystine transport leading to oxidative stress. Neuron. 2: 1547-1558. https://doi.org/10.1016/0896-6273(89)90043-3
  22. Park, S. J., Park, D. S., Lee, S. B., He, X., Ahn, J. H., Yoon, W. B. and Lee, H. Y. (2010) Enhancement of antioxidant activities of Codonopsis lanceolata and fermented Codonopsis lanceolata by ultra high pressure extraction. J. Korean Soc. Food Sci. Nutr. 39: 1898-1902.