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The antidiabetic effects of an herbal formula composed of Alnus hirsuta, Rosa davurica, Acanthopanax senticosus and Panax schinseng in the streptozotocin-induced diabetic rats

  • Hu, Weicheng ;
  • Yeo, Jin-Hee ;
  • Jiang, Yunyao ;
  • Heo, Seong-Il ;
  • Wang, Myeong-Hyeon
  • Received : 2012.08.09
  • Accepted : 2013.01.04
  • Published : 2013.04.01

Abstract

A folk prescription consisting of Alnus hirsuta, Rosa davurica, Acanthopanax senticosus and Panax schinseng has been used in the treatment of diabetes mellitus. The aim of the present investigation was to evaluate the antidiabetic effects of the herb formula extract (HFE) composed of Alnus hirsuta, Rosa davurica, Acanthopanax senticosus and Panax schinseng in the streptozotocin (STZ)-induced diabetic rats. The HFE was mixed in the food supply of the healthy and STZ-induced diabetic male Sprague-Dawley rats, and its effects on the body weight, water and food intake, hyperglycemia, hypolipidemic and islet structure were studied. The treatment of the rats with STZ for 6 weeks resulted in marasmus, polydipsia, polyphagia, hyperglycemia and hypoinsulinemia. In addition, the diabetic rats showed an apparent decrease in the insulin immunoreactivity and the number of ${\beta}$-cells in the pancreas. The addition of the HFE to the rats' food supply significantly lowered the serum glucose and the serum triglycerides level and preserved the normal histological appearance of the pancreatic islets. These results indicate that the HEF have a strong antidiabetic potential along with the significant hypoglycemic and hypolipidemic effects, which may be applicable in the pharmaceutical industry.

Keywords

Antidiabetic;hyperglycemia;immunohistochemistry;multi-herbal formula;streptozotocin

References

  1. Mentreddy SR. Medicinal plant species with potential antidiabetic properties. J Sci Food Agric 2007;87:743-50. https://doi.org/10.1002/jsfa.2811
  2. Heo SI, Jin YS, Jung MJ, Wang MH. Antidiabetic properties of 2,5-dihydroxy-4,3'-di(beta-D-glucopyranosyloxy)-trans-stilbene from mulberry (Morus bombycis Koidzumi) root in streptozotocininduced diabetic rats. J Med Food 2007;10:602-7. https://doi.org/10.1089/jmf.2006.0241
  3. Lebovitz HE. Etiology and pathogenesis of diabetes mellitus. Pediatr Clin North Am 1984;31:521-30. https://doi.org/10.1016/S0031-3955(16)34604-1
  4. Gurib-Fakim A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med 2006;27:1-93. https://doi.org/10.1016/j.mam.2005.07.008
  5. Dennis JC, Coleman ES, Swyers SE, Moody SW, Wright JC, Judd R, Zhong Q, Morrison EE. Changes in mitotic rate and GFAP expression in the primary olfactory axis of streptozotocininduced diabetic rats. J Neurocytol 2005;34:3-10. https://doi.org/10.1007/s11068-005-5044-x
  6. Celik S, Erdogan S, Tuzcu M. Caffeic acid phenethyl ester (CAPE) exhibits significant potential as an antidiabetic and liverprotective agent in streptozotocin-induced diabetic rats. Pharmacol Res 2009;60:270-6. https://doi.org/10.1016/j.phrs.2009.03.017
  7. Pari L, Saravanan G. Antidiabetic effect of Cogent db, a herbal drug in alloxan-induced diabetes mellitus. Comp Biochem Physiol C Toxicol Pharmacol 2002;131:19-25. https://doi.org/10.1016/S1532-0456(01)00259-9
  8. Stirban AO, Tschoepe D. Cardiovascular complications in diabetes: targets and interventions. Diabetes Care 2008;31 Suppl 2:S215-21. https://doi.org/10.2337/dc08-s257
  9. Despres JP, Lemieux I, Dagenais GR, Cantin B, Lamarche B. HDL-cholesterol as a marker of coronary heart disease risk: the Quebec cardiovascular study. Atherosclerosis 2000;153:263-72. https://doi.org/10.1016/S0021-9150(00)00603-1
  10. Badole SL, Bodhankar SL. Antidiabetic activity of cycloart-23- ene-$3\beta$, 25-diol (B2) isolated from Pongamia pinnata (L. Pierre) in streptozotocin-nicotinamide induced diabetic mice. Eur J Pharmacol 2010;632:103-9. https://doi.org/10.1016/j.ejphar.2010.01.019
  11. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003;79:829-43. https://doi.org/10.1016/S0015-0282(02)04948-8
  12. Kishore A, Nampurath GK, Mathew SP, Zachariah RT, Potu BK, Rao MS, Valiathan M, Chamallamudi MR. Antidiabetic effect through islet cell protection in streptozotocin diabetes: a preliminary assessment of two thiazolidin-4-ones in Swiss albino mice. Chem Biol Interact 2009;177:242-6. https://doi.org/10.1016/j.cbi.2008.10.032
  13. Norquay LD, D'Aquino KE, Opare-Addo LM, Kuznetsova A, Haas M, Bluestone JA, White MF. Insulin receptor substrate-2 in $\beta$-cells decreases diabetes in nonobese diabetic mice. Endocrinology 2009;150:4531-40. https://doi.org/10.1210/en.2009-0395
  14. Singh N, Kamath V, Rajini PS. Attenuation of hyperglycemia and associated biochemical parameters in STZ-induced diabetic rats by dietary supplementation of potato peel powder. Clin Chim Acta 2005;353:165-75. https://doi.org/10.1016/j.cccn.2004.10.016
  15. Hu W, Wang MH. Diarylheotanoid from Alnus hirsuta improves glucose metabolism via insulin signal transduction in human hepatocarcinoma (HepG2) cells. Biotechnol Bioprocess Eng 2011; 16:120-6. https://doi.org/10.1007/s12257-010-0311-9
  16. Xing XH, Zhang ZM, Hu XZ, Wu RQ, Xu C. Antidiabetic effects of Artemisia sphaerocephala Krasch. gum, a novel food additive in China, on streptozotocin-induced type 2 diabetic rats. J Ethnopharmacol 2009;125:410-6. https://doi.org/10.1016/j.jep.2009.07.021
  17. Skelly AH. Type 2 diabetes mellitus. Nurs Clin North Am 2006; 41:531-47. https://doi.org/10.1016/j.cnur.2006.07.011
  18. Bailes BK. Diabetes mellitus and its chronic complications. AORN J 2002;76:266-76, 278-82.
  19. Baudry A, Leroux L, Jackerott M, Joshi RL. Genetic manipulation of insulin signaling, action and secretion in mice. Insights into glucose homeostasis and pathogenesis of type 2 diabetes. EMBO Rep 2002;3:323-8. https://doi.org/10.1093/embo-reports/kvf078
  20. Ceriello A. New insights on oxidative stress and diabetic complications may lead to a "causal" antioxidant therapy. Diabetes Care 2003;26:1589-96. https://doi.org/10.2337/diacare.26.5.1589
  21. Chang MS, Oh MS, Kim DR, Jung KJ, Park S, Choi SB, Ko BS, Park SK. Effects of okchun-san, a herbal formulation, on blood glucose levels and body weight in a model of type 2 diabetes. J Ethnopharmacol 2006;103:491-5. https://doi.org/10.1016/j.jep.2005.08.039
  22. Vetrichelvan T, Jegadeesan M. Anti-diabetic activity of alcoholic extract of Aerva lanata (L.) Juss. ex Schultes in rats. J Ethnopharmacol 2002;80:103-7. https://doi.org/10.1016/S0378-8741(01)00412-3
  23. Westphal SA, Palumbo PJ. Insulin and oral hypoglycemic agents should not be used in combination in the treatment of type 2 diabetes. Arch Intern Med 2003;163:1783-5. https://doi.org/10.1001/archinte.163.15.1783
  24. Chien SC, Young PH, Hsu YJ, Chen CH, Tien YJ, Shiu SY, Li TH, Yang CW, Marimuthu P, Tsai LF, Yang WC. Antidiabetic properties of three common Bidens pilosa variants in Taiwan. Phytochemistry 2009;70:1246-54. https://doi.org/10.1016/j.phytochem.2009.07.011
  25. Modak M, Dixit P, Londhe J, Ghaskadbi S, Paul A Devasagayam T. Indian herbs and herbal drugs used for the treatment of diabetes. J Clin Biochem Nutr 2007;40:163-73. https://doi.org/10.3164/jcbn.40.163

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  2. by HPLC with charged aerosol detection and confirmation by LC-ESI-TOF-MS vol.39, pp.12, 2016, https://doi.org/10.1002/jssc.201501029

Acknowledgement

Supported by : Kangwon National University