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Optimization of Bio-based Succinic Acid Production from Hardwood Using the Two Stage pretreatments

  • Jung, Ji Young (Division of Environmental Forest Science, Gyeongsang National University, Institute of Agriculture & Life Sciences) ;
  • Jo, Jong Soo (Gyeongnam National University of Science and Technology, Department of Interior Materials Engineering) ;
  • Kim, Young Wun (Korea Research Institute of Chemical Technology) ;
  • Yoon, Byeng Tae (Korea Research Institute of Chemical Technology) ;
  • Kim, Choon Gil (SK Energy Institute of Technology) ;
  • Yang, Jae Kyung (Division of Environmental Forest Science, Gyeongsang National University, Institute of Agriculture & Life Sciences)
  • Received : 2012.11.23
  • Accepted : 2013.03.07
  • Published : 2013.03.25

Abstract

The steam explosion-chemical pretreatment is a more effective wood pretreatment technique than the conventional physical pretreatment by accelerating reactions during the pretreatment process. In this paper, two-stage pretreatment processes of hardwood were investigated for its enzymatic hydrolysis and the succinic acid yield from the pretreated solid. The first stage pretreatment was performed under conditions of low severity to optimize the amount of solid recovery. In the second stage pretreatment washed solid material from the first stage pretreatment step was impregnated again with chemical (alkaline or chlorine-based chemicals) to remove a portion of the lignin, and to make the cellulose more accessible to enzymatic attack. The effects of pretreatment were assessed by enzymatic hydrolysis and fermentation, after the two stage pretreatments. Maximum succinic acid yield (16.1 g $L^{-1}$ and 77.5%) was obtained when the two stage pretreatments were performed at steam explosion -3% KOH.

Keywords

Acknowledgement

Supported by : Korea Forest Service, Ministry of Knowledge Economy

References

  1. Badger, P. C. 2002. Ethanol from cellulose: a general review. In: Janick, J., Whipkey, A. (Eds.), Trends in New Crops and New Uses. ASHS Press, Alexandria, VA, 17-21.
  2. Chang, V. S. and M. T. Holtzapple. 2000. Fundamental factors affecting enzymatic reactivity. Applied Biochemistry and Biotechnology 84-86: 5-37. https://doi.org/10.1385/ABAB:84-86:1-9:5
  3. Converti, A. and M. D. Borghi. 1998. Inhibition of the fermentation of oak hemicellulose acid hydrolysate by minor sugars. Journal of Biotechnology 64: 211-218. https://doi.org/10.1016/S0168-1656(98)00109-6
  4. De Bari, I., Viola, E., Barisano, D., Cardinale, M., Nanna, F., Zimbardi, F., Cardinale, G., and G. Braccio. 2002. Ethanol production at flask and pilot scale from concentrated slurries of steamexploded aspen. Industrial & Engineering Chemistry Research 41: 1745-1753. https://doi.org/10.1021/ie010571f
  5. Duff, S. J. B. and W. D. Murray. 1996. Bioconversion of forest products industry waste cellulosic to fuel ethanol: A Review. Bioresource Technology 55: 1-33. https://doi.org/10.1016/0960-8524(95)00122-0
  6. Fan, L. T., Gharpuray, M. M., and Y. H. Lee. 1987. In: Cellulose hydrolysis biotechnology monographs. Springer, Berlin, p. 57.
  7. Feist, W. C., baker A. J., and H. Tarkow. 1970. Alklai requirements for improving digestibility of hardwoods by rumen micro-organisms. Journal of Animal Science 30: 832-836. https://doi.org/10.2527/jas1970.305832x
  8. Felizon, B., Fernandez-Bolanos, J., Heredia, A., and R. Guillen. 2000. Steam explosion pretreatment of olive cake. Journal of the American Oil Chemists' Society 77: 15-22. https://doi.org/10.1007/s11746-000-0003-y
  9. Fernandez-Bolanos, J., Felizon, B., Heredia, A., Guillen, R., and A. Jimenez. 1999. Characterization of the lignin obtained by alkaline delignification and of the cellulose residue from steam exploded olive stones. Bioresource Technology 68: 121-132. https://doi.org/10.1016/S0960-8524(98)00134-5
  10. Hendriks, A. T. W. M. and G. Zeeman. 2009. Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technology 100: 10-18.
  11. Iyer P. V. and Y. Y. Lee.1999. Product inhibition in simultaneous saccharification and fermentation of cellulose into lactic acid. Biotechnology Letters 21: 371-373. https://doi.org/10.1023/A:1005435120978
  12. Jackobsons, J., Hortling, B., Erins, P., and J. Sundquist. 1995. Characterization of alkali soluble fraction of steam exploded birch wood. Holzforschung 49(1): 51-59. https://doi.org/10.1515/hfsg.1995.49.1.51
  13. Jeoh, T. Steam explosion pretreatment of cotton gin waste for fuel ethanol production. Master's thesis, Virginia Tech. University, VA, 1998.
  14. Jorgensen, H., Kristensen, J. B., and C. Felby. 2007. Enzymatic conversion of lignocellulose into fermentable sugars: Challenges and opportunities. Biofuels, Bioproducts and Biorefining 1: 119-134. https://doi.org/10.1002/bbb.4
  15. Kabel, M. A., Bos, G., Zeevalking, J., Voragen, A. G., and H. A. Schols. 2007. Effect of pretreatment severity on xylan solubility and enzymatic breakdown of the remaining cellulose from wheat straw. Bioresource Technology 98: 2034-2042. https://doi.org/10.1016/j.biortech.2006.08.006
  16. Kallavus, U. and Gravitis. J. 1995. A comparative investigation of the ultrastructure of steam exploded wood with light, scanning and transmission electron microscopy. Holzforschung 49(2): 182-188. https://doi.org/10.1515/hfsg.1995.49.2.182
  17. Kim, D. Y., Yim, S. C., Lee, P. C., Lee, W. G., Lee, S. Y., and H. N. Chang. 2004. Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens MBEL55E. Enzyme and Microbial Technology 35: 648-653. https://doi.org/10.1016/j.enzmictec.2004.08.018
  18. Lee, W. G., Lee, J. S., Shin, C. S., Park, S. C., Chang, H. N., andY. K. Chang. 1999. Ethanol production using concentrated oak wood hyrolysates and methods to detoxify. Applied Biochemistry and Biotechnology 77-79: 547-559.
  19. Li, J., Lennholm, H., Henriksson, G., and G. Gellerstedt. 2001. Bio-refinery of lignocellulosic materials for ethanol production. II. Fundaments and strategic design of steam explosion. In: Kyritsis S, Beenackers AACM, Helm P, Grassi A, Chiaramonti D, editors. Proceedings of the firs world conference on biomass for energy and industry 1: 767-770.
  20. Li, J., Henriksson, G., and G. Gellerstedt. 2007. Lignin depolymerization/repolymerization and its critical role for delignification of aspen wood by steam explosion. Bioresource Technology 98: 3061-3068. https://doi.org/10.1016/j.biortech.2006.10.018
  21. Liu, Y. P., Zheng, P., Sun, Z. H., Ni, Y., Dong, J. J., and L. L. Zhu. 2008. Economical succinic acid production from cane molasses by Actinobacillus succinogenes. Bioresource Technology 99: 1736-1742. https://doi.org/10.1016/j.biortech.2007.03.044
  22. Milne, T. A., Chum, H. L., Agblevor, F. A., and D. K. Johnson. 1992. Standardized analytical methods. Biomass and Bioenergy 2: 341-366. https://doi.org/10.1016/0961-9534(92)90109-4
  23. Mosier, N., Wyman, C., Dale, B., Elander, R. Lee, Y. Y., Holtzapple, M., and M. Ladisch. 2005. Features of promising technologies for pretreatment of lignocellulosic biomass, Bioresource Technology 96: 673-686. https://doi.org/10.1016/j.biortech.2004.06.025
  24. Moniruzzaman, M. 1996. Effect of steam explosion on the physicochemical properties and enzymatic saccharification of rice straw. Applied Biochemistry and Biotechnology 59: 283-297. https://doi.org/10.1007/BF02783570
  25. Palmqvist. E. and B. Hahn-Hagerdal. 2000. Fermentation of lignocellulosic hydrolysates. I: Inhibition and detoxification. Bioresource Technology 74: 17-24. https://doi.org/10.1016/S0960-8524(99)00160-1
  26. Ramos, L. P., Breuil, C., Kushner, D. N., and J. N. Saddler. 1992. Steampretreatment conditions for effective enzymatic hydrolysis and recovery yields of Eucalyptus viminalis wood chip. Holzforschung 46: 149-154. https://doi.org/10.1515/hfsg.1992.46.2.149
  27. Soderstrom, J., Pilcher, L., Galbe, M., and G. Zacchi. 2003. Two-step pretreatment of softwood by dilute H2SO4 impregnation for ethanol production. Biomass and Bioenergy 24: 475-486. https://doi.org/10.1016/S0961-9534(02)00148-4
  28. Sun, Y. and J. Cheng. 2002. Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresource Technology 83: 1-11. https://doi.org/10.1016/S0960-8524(01)00212-7
  29. Take, H., Andou, Y., Nakamura, Y., Kobayashi, F., Kurimoto, Y., and M. Kuwahara. 2006. Production of methane gas from Japanese cedar chips pretreated by various delignification methods. Biochemical Engineering Journal 28: 30-35. https://doi.org/10.1016/j.bej.2005.08.036
  30. Tarkow, H. and W. C. Feist. 1969. In: A Mechanism for Improving the Digestibility of Lignocellulosic Materials with Dilute Alkali and Liquid NH3 Advance Chemistry Series 95. American Chemical Society, Washington, DC, 197-218.
  31. Taylor, J. D. and E. K. C. Yu. 1995. Continuous Steam Explosion. Chemtech-American Chernical Society 2: 38-41.
  32. Tengborg, C., Galbe, M., and G. Zacchi. 2001. Reduced inhibition of enzymatic hydrolysis of steam-pretreated softwood. Enzyme and Microbial Technology 28: 835-844. https://doi.org/10.1016/S0141-0229(01)00342-8
  33. Zeikus, J. G., Jain, M. K., and P. Elankovan. 1999. Biotechnology of succinic acid production and markets for derived industrial products. Applied Microbiology and Biotechnology 51: 545-552 https://doi.org/10.1007/s002530051431
  34. Zheng, P., Dong, J. J., Sun, Z. H., Ni, Y., and L. Fang. 2009. Fermentative production of succinic acid from straw hydrolysate by Actinobacillus succinogenes. Bioresource Technology 100: 2425-2429. https://doi.org/10.1016/j.biortech.2008.11.043
  35. Zheng, P., Fang, L., Xu, Y., Dong, J. J., Ni, Y., and Z. H. Sun. 2010. Succinic acid production from corn stover by simultaneous saccharification and fermentation using Actinobacillus succinogenes. Bioresource Technology 101: 7889-7894. https://doi.org/10.1016/j.biortech.2010.05.016
  36. Zhu, J. Y. and X. J. Pan. 2010. Woody biomass pretreatment for cellulosic ethanol production: technology and energyconsumption evaluation. Bioresource Technology 101: 4992-5002. https://doi.org/10.1016/j.biortech.2009.11.007
  37. Ucar, G. 1990. Pretreatment of poplar by acid and alkali for enzymatic hydrolysis. Wood Science and Technology 24: 171-180.