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Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells

  • Lee, Young-Rae (Department of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • Noh, Eun-Mi (Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School) ;
  • Han, Ji-Hey (Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School) ;
  • Kim, Jeong-Mi (Department of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • Hwang, Bo-Mi (Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School) ;
  • Kim, Byeong-Soo (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Sung-Ho (Department of Companion and Laboratory Animal Science, Kongju National University) ;
  • Jung, Sung Hoo (Division of Breast Thyroid Surgery, Department of Surgery, Chonbuk National University Medical School) ;
  • Youn, Hyun Jo (Division of Breast Thyroid Surgery, Department of Surgery, Chonbuk National University Medical School) ;
  • Chung, Eun Yong (Department of Anestheiology and Pain Medicine, St. Mary's Hospital, College of Medicine, The Catholic University of Korea) ;
  • Kim, Jong-Suk (Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University Medical School)
  • Received : 2012.08.02
  • Accepted : 2012.11.30
  • Published : 2013.04.30

Abstract

Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is an isothiocyanate found in some cruciferous vegetables, especially broccoli. Sulforaphane has been shown to display anti-cancer properties against various cancer cell lines. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. In this study, we investigated the effect of sulforaphane on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion in MCF-7 cells. TPA-induced MMP-9 expression and cell invasion were decreased by sulforaphane treatment. TPA substantially increased NF-${\kappa}B$ and AP-1 DNA binding activity. Pre-treatment with sulforaphane inhibited TPA-stimulated NF-${\kappa}B$ binding activity, but not AP-1 binding activity. In addition, we found that sulforaphane suppressed NF-${\kappa}B$ activation, by inhibiting phosphorylation of $I{\kappa}B $ in TPA-treated MCF-7 cells. In this study, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion by sulforaphane was mediated by the suppression of the NF-${\kappa}B$ pathway in MCF-7 cells.

Keywords

Invasion;MCF-7;MMP-9;NF-${\kappa}B$;Sulforaphane

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