DOI QR코드

DOI QR Code

Effect of Oral Administration of Intact Casein on Gastrointestinal Hormone Secretion and Pancreatic α-Amylase Activity in Korean Native Steer

  • Lee, S.B. ;
  • Choi, C.W. ;
  • Jin, Y.C. ;
  • Wang, T. ;
  • Lee, K.H. ;
  • Ku, M.B. ;
  • Hwang, J.H. ;
  • Kim, K.H. ;
  • Vega, R.S.A. ;
  • Lee, H.G.
  • Received : 2012.09.18
  • Accepted : 2012.11.15
  • Published : 2013.05.01

Abstract

Three Korean native steers ($779{\pm}24$ kg) fitted with duodenal cannulas were used in a $3{\times}3$ Latin square design to investigate the influence of oral administration of soluble proteins, intact casein (IC) and acid hydrolyzed casein (AHC), on gastro-intestinal hormone (GIH) secretion in the blood and pancreatic ${\alpha}$-amylase activity in the duodenum. Oral treatment consisted of a basic diet (control), IC (C+100% protein), or AHC (C+80% amino acid, 20% peptide) for 21 d. Blood and duodenum samples were collected for measurement of serum GI hormones, and pancreatic ${\alpha}$-amylase activity was determined at 900, 1030, 1330, 1630, and 1930 h after feeding on d 21 of treatment. The levels of serum cholecystokinin (CCK) and secretin in the IC treatment group were higher compared to the other treatment groups (p<0.05). In addition to the changes in CCK and secretin levels upon IC treatment, the pancreatic ${\alpha}$-amylase activity in the duodenum was higher in the IC group compared to the control diet group (p<0.05). The response of serum ghrelin to IC and AHC treatment was in accordance with the response of serum secretin. The level of peptide fragments flowing in the duodenum was higher in the IC treatment group than the other treatment groups (p<0.05). In conclusion, this study demonstrated that an increase in duodenal CCK and secretin upon IC oral administration increased pancreatic ${\alpha}$-amylase secretion. In addition, ghrelin may be associated with GI hormone secretion in Korean native steers.

Keywords

Secretin;Cholecystokinin;Ghrelin;${\alpha}$-Amylase;Korean Native Steer

References

  1. Rausch, U., P. Vasiloudes, K. Rudiger and H. F. Kern. 1985. In-vivo stimulation of rat pancreatic acinar cells by infusion of secretin. 1. Changes in enzyme content, pancreatic fine structure and total rate of protein synthesis. Cell Tissue Res. 242:633-639. https://doi.org/10.1007/BF00225430
  2. Relling, A. E. and C. K. Reynolds. 2008. Abomasal infusion of casein, starch and soybean oil differentially affects plasma concentrations of gut peptides and feed intake in lactating dairy cows. Domest. Anim. Endocrinol. 35:35-45. https://doi.org/10.1016/j.domaniend.2008.01.005
  3. Richards, C. J., A. F. Branco, D. W. Bohnert, G. B. Huntington, M. Macari and D. L. Harmon. 2002. Intestinal starch disappearance increased in steers abomasally infused with starch and protein. J. Anim. Sci. 80:3361-3368.
  4. Richards, C. J., K. C. Swanson, S. J. Paton, D. L. Harmon and G. B. Huntington. 2003. Pancreatic exocrine secretion in steers infused post-ruminally with casein and corn starch. J. Anim. Sci. 81:1051-1056.
  5. Sun, Y., P. Wang, H. Zheng and R. G. Smith. 2004. Ghrelin stimulation of growth hormone release and appetite is mediated through the growth hormone secretagogue receptor. PNAS. 101:4679-4684. https://doi.org/10.1073/pnas.0305930101
  6. Swanson, K. C., J. A. Benson, J. C. Matthews and D. L. Harmone. 2004. Pancreatic exocrine secretion and plasma concentration of some gastrointestinal hormones in response to abomasal infusion of starch hydrolyzate and/or casein. J. Anim. Sci. 82:1781-1787.
  7. Swanson, K. C., J. C. Matthews, C. A. Woods and D. L. Harmon. 2002. Post-ruminal administration of partially hydrolyzed starch and casein influences pancreatic $\alpha$-amylase expression in calves. J. Nutr. 132:376-381.
  8. Tachibana, S., T. Onaga, H. Mineo and S. Kato. 1995. Role of endogenous CCK in regulation of interdigestive pancreatic exocrine secretion in sheep (Ovis aries). Comp. Biochem. Physiol. A. Physiol. 112:103-109. https://doi.org/10.1016/0300-9629(95)00083-J
  9. Taniguchi, K., G. B. Huntington and B. P. Glenn. 1995. Net nutrient flux by visceral tissues of beef steers given abomasal and ruminal infusions of casein and starch. J. Anim. Sci. 73:236-249.
  10. Than Than, S., H. Zhao, S. Yannaing, T. Ishikawa and H. Kuwayama. 2010. Oxyntomodulin increases the concentrations of insulin and glucose in plasma but does not affect ghrelin secretion in Holstein cattle under normal physiological conditions. Domest. Anim. Endocrinol. 39:163-170. https://doi.org/10.1016/j.domaniend.2010.05.001
  11. Harmon, D. L. 1992. Dietary influences on carbohydrases and small intestinal starch hydrolysis capacity in ruminants. J. Nutr. 122:203-210.
  12. Harmon, D. L. and C. C. Taylor. 2005. Factors influencing assimilation of dietary starch in beef and dairy cattle. Proc. Southwest Nutr. Conf. 55-66.
  13. Hashimoto, N. and H. Hara. 2004. Dietary branched-chain amino acids suppress the expression of pancreatic amylase mRNA in rats. Biosci. Biotechnol. Biochem. 68:1067-1072. https://doi.org/10.1271/bbb.68.1067
  14. Huhtanen, P., P. G. Brotz and L. D. Satter. 1997. Omasal sampling technique for assessing fermentative digestion in the forestomach of dairy cow. J. Anim. Sci. 75:1380-1392.
  15. Itoh, F. A., K. Tokushi, K. Shiro and H. Koichi. 2006. Effects of ghrelin injection on plasma concentrations of glucose, pancreatic hormones and cortisol in Holstein dairy cattle. Comp. Biochem. Physiol. A. Mol. Integr. Physiol. 143:97-102. https://doi.org/10.1016/j.cbpa.2005.11.001
  16. Langlois, A., T. Corring, J. C. Cuber, A. M. Gueugneau, F. Levenez and J. A. Chayvialle. 1989. Effects of Pancreatic polypeptide on the pancreatic exocrine secretion stimulated by secretin and cholecystokinin in the conscious pig. Regul. Pept. 24:55-65. https://doi.org/10.1016/0167-0115(89)90211-5
  17. Lin, T. M., D. C. Evans, R. E. Chance and G. F. Spray. 1977. Bovine pancreatic polypeptide: action on gastric and pancreatic secretion in dogs. Am. J. Physiol. 232:311-315.
  18. Li, Y. and C. Owyang. 1993. Vagal afferent pathway mediates physiological action of cholecystokinin on pancreatic enzyme secretion. J. Clin. Invest. 92:418-424. https://doi.org/10.1172/JCI116583
  19. Manso, M. A., J. I. San Roman, I. de Dios, L. J. Garcia and M. A. Lopez. 1989. Effect of secretin on pancreatic juice proteins in caerulein induced acute pancreatitis in the rat. Peptides. 10:255-260. https://doi.org/10.1016/0196-9781(89)90027-2
  20. Meyer, J. H., L. J. Spingola and M. I. Grossman. 1971. Endogenous cholecystokinin potentiates exogenous secretin on pancreas of dog. Am. J. Physiol. 221:742-747.
  21. National Institute of Animal Science (NIAS). 2007. Korean Feeding Standard for Hanwoo. RDA, Suwon.
  22. Nishi, T., H. Hara and T. Kasai. 1998. Guanidinated casein hydrolysate stimulates pancreatic secretagogue release by direct action to the intestine in rats. Proc. Soc. Exp. Biol. Med. 218:357-364. https://doi.org/10.3181/00379727-218-44304
  23. Choi, C. W., A. Vanhatalo, S. Ahvenjarvi and P. Huhtanen. 2002. Effects of several protein supplements on flow of soluble non-ammonia nitrogen from the forestomach and milk production in dairy cows. Anim. Feed Sci. Technol. 102:15-33. https://doi.org/10.1016/S0377-8401(02)00251-1
  24. Choi, C. W., K. H. Kim, S. S. Chang and N. J. Choi. 2012. Soluble non-ammonia nitrogen in ruminal and omasal digesta of Korean native steers supplemented with soluble proteins. Asian Australas. J. Anim. Sci. 25:1269-1275. https://doi.org/10.5713/ajas.2012.12318
  25. Cordier-Bussat, M., C. Bernard, S. Haouche, C. Roche, J. Abello, J. A. Chayvialle and J. C. Cuber. 1997. Peptones stimulate cholecystokinin secretion and gene transcription in the intestinal cell line STC-1. Endocrinology 138:1137-1144. https://doi.org/10.1210/en.138.3.1137
  26. Croom, W. J. Jr., L. S. Bull and I. L. Taylor. 1992. Regulation of pancreatic exocrine secretion in ruminants: A review. J. Nutr. 122:191-202.
  27. Eastwood, C., K. Maubach, A. J. Kirkup and D. Grundy. 1998. The role of endogenous cholecystokinin in the sensory transduction of luminal nutrient signals in the rat jejunum. Neurosci. Lett. 254:145-148. https://doi.org/10.1016/S0304-3940(98)00666-1
  28. Folsch, U. R., A. Oldendorp, P. G. Lankish and W. Creutzfeldt. 1984. European Journal of Clinical Investigation. 14(Suppl): 45.
  29. Go, V. L., A. F. Hofmann and W. H. Summerskill. 1970. Pancreozymin bioassay in man based on pancreatic enzyme secretion: potency of specific amino acids and other digestive products. J. Clin. Invest. 49:1558-1564. https://doi.org/10.1172/JCI106373
  30. Greenberg, G. R., R. F. McCloy, V. S. Chadwick, T. E. Adrian, J. H. Baron and S. R. Bloom. 1979. Effect of bovine pancreatic polypeptide on basal pancreatic and biliary out puts in man. Dig. Dis. Sci. 24:11-14. https://doi.org/10.1007/BF01297231
  31. Hara, H., S. Ohyama and T. Hira. 2000. Luminal dietary protein, not amino acids, induces pancreatic protease via CCK in pancreaticobiliary-diverted rats. Am. J. Physiol. 278:937-945.
  32. Chance, R. E., M. Gieszkowski, J. Jaworek, S. J. Konturek, J. Swierczek and J. Tasler. 1981. Effect of pancreatic polypeptide and its C-terminal hexapeptide on meal and secredn induced pancreatic accretion in dogs. J. Physiol. 314:1-9. https://doi.org/10.1113/jphysiol.1981.sp013685

Acknowledgement

Supported by : Rural Development Administration