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Investigation of MC1R SNPs and Their Relationships with Plumage Colors in Korean Native Chicken

  • Hoque, M.R. ;
  • Jin, S. ;
  • Heo, K.N. ;
  • Kang, B.S. ;
  • Jo, C. ;
  • Lee, J.H.
  • Received : 2012.10.19
  • Accepted : 2013.01.11
  • Published : 2013.05.01

Abstract

The melanocortin 1 receptor (MC1R) gene is related to the plumage color variations in chicken. Initially, the MC1R gene from 30 individuals was sequenced and nine polymorphisms were obtained. Of these, three and six single nucleotide polymorphisms (SNPs) were confirmed as synonymous and nonsynonymous mutations, respectively. Among these, three selected SNPs were genotyped using the restriction fragment length polymorphism (RFLP) method in 150 individuals from five chicken breeds, which identified the plumage color responding alleles. The neighbor-joining phylogenetic tree using MC1R gene sequences indicated three well-differentiated different plumage pigmentations (eumelanin, pheomelanin and albino). Also, the genotype analyses indicated that the TT, AA and GG genotypes corresponded to the eumelanin, pheomelanin and albino plumage pigmentations at nucleotide positions 69, 376 and 427, respectively. In contrast, high allele frequencies with T, A and G alleles corresponded to black, red/yellow and white plumage color in 69, 376 and 427 nucleotide positions, respectively. Also, amino acids changes at position Asn23Asn, Val126Ile and Thr143Ala were observed in melanin synthesis with identified possible alleles, respectively. In addition, high haplotype frequencies in TGA, CGG and CAA haplotypes were well discriminated based on the plumage pigmentation in chicken breeds. The results obtained in this study can be used for designing proper breeding and conservation strategies for the Korean native chicken breeds, as well as for the developing breed identification markers in chicken.

Keywords

Korean Native Chicken;MC1R;Plumage Color;SNPs

References

  1. Thompson, J. D., D. G. Higgins, T. J. Gibson and W. Clustal. 1994. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680. https://doi.org/10.1093/nar/22.22.4673
  2. Tixier-Biochard, M. 2011. What can genomics tell us about chicken domestication and selection? 9th A. P. P. C. 32-41.
  3. Vage, D. I., H. Klungland, D. Lu and R. D. Cone. 1999. Molecular and pharmacological characterization of dominant black coat color in sheep. Mamm. Genome 10:39-43. https://doi.org/10.1007/s003359900939
  4. Vage, D. I., D. Lu, H. Klungland, S. Lien, S. Adalsteinsson and R. D. Cone. 1997. A nonepistatic interaction of agouti and extension in the fox, Vulpes vulpes. Nat. Genet. 15:311-315. https://doi.org/10.1038/ng0397-311
  5. Vidal, O., R. M. Araguas, R. Fernandez, S. Heras, N. Sanz and C. Pla. 2010. Melanism in guinea fowl (Numida meleagris) is associated with a deletion of Phenylalanine-256 in the MC1R gene. Anim. Genet. 41:656-658. https://doi.org/10.1111/j.1365-2052.2010.02056.x
  6. Hoekstra, H. E. 2006. Genetics, development and evolution of adaptive pigmentation in vertebrates. Heredity 97:222-234. https://doi.org/10.1038/sj.hdy.6800861
  7. Jackson, I. J. 1997. Homologous pigmentation mutations in human, mouse and other model organisms. Hum. Mol. Genet. 6:1613-1624. https://doi.org/10.1093/hmg/6.10.1613
  8. Jackson, I. J., P. Budd, J. M. Horn, R. Johnson, S. Raymond and K. Steel. 1994. Genetics and molecular biology of mouse pigmentation. Pigment Cell Res. 7:73-80. https://doi.org/10.1111/j.1600-0749.1994.tb00024.x
  9. Kerje, S., J. Lind, K. Schutz, P. Jensen and L. Andersson. 2003. Melanocortin 1-receptor (MC1R) mutations are associated with plumage colour in chicken. Anim. Genet. 34:241-248. https://doi.org/10.1046/j.1365-2052.2003.00991.x
  10. Kijas, J. M. H., M. Moller, G. Plastow and L. Andersson. 2001. A frameshift mutation in MC1R and a high frequency of somatic reversions cause black spotting in pigs. Genetics 158:779-785.
  11. Kijas, J. M. H., R. Wales, A. Tornsten, P. Chardon, M. Moller and L. Andersson. 1998. Melanocortin receptor 1 (MC1R) mutations and coat color in pigs. Genetics 150:1177-1185.
  12. Klungland, H., D. I. Vage, L. Gomez-Raya, S. Adalsteinsson and S. Lien. 1995. The role of melanocyte-stimulating hormone (MSH) receptor in bovine coat color determination. Mamm. Genome 6:636-639. https://doi.org/10.1007/BF00352371
  13. Kumar, S., M. Nei, J. Dudley and K. Tamura. 2008. MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform. 9:299-306. https://doi.org/10.1093/bib/bbn017
  14. Ling, M. K., M. C. Lagerstrom, R. Fredriksson, R. Okimoto, N. I. Mundy, S. Takeuchi and H. B. Schioth. 2003. Association of feather colour with constitutively active melanocortin 1 receptors in chicken. Eur. J. Biochem. 270:1441-1449. https://doi.org/10.1046/j.1432-1033.2003.03506.x
  15. Marklund, L., M. J. Moller, K. Sandberg and L. Andersson. 1996. A missense mutation in the gene for melanocyte-stimulating hormone receptor (MC1R) is associated with the chestnut coat color in horses. Mamm. Genome 7:895-899. https://doi.org/10.1007/s003359900264
  16. Nadeau, N. J., F. Minvielle and N. I. Mundy. 2006. Association of a Glu92Lys substitution in MC1R with extended brown in Japanese quail (Coturnix japonica). Anim. Genet. 37:287-289. https://doi.org/10.1111/j.1365-2052.2006.01442.x
  17. Nadeau, N. J., T. Burke and N. I. Mundy. 2007. Evolution of an avian pigmentation gene correlates with a measure of sexual selection. Proc. Biol. Sci. 274:1807-1813. https://doi.org/10.1098/rspb.2007.0174
  18. Newton, J. M., A. L. Wilkie, L. He, S. A. Jordan, D. L. Metallinos, N. G. Holmes, I. J. Jackson and G. S. Barsh. 2000. Melanocortin 1 receptor variation in the domestic dog. Mamm. Genome 11:24-30. https://doi.org/10.1007/s003350010005
  19. Robbins, L. S., J. H. Nadeau, K. R. Johnson, M. A. Kelly, L. Roselli-Rehfuss, E. Baack, K. G. Mountjoy and R. D. Cone. 1993. Pigmentation phenotypes of variant extension locus alleles results from point mutations that alter MSH receptor function. Cell 72:827-834. https://doi.org/10.1016/0092-8674(93)90572-8
  20. Takeuchi, S., H. Suzuki, M. Yabuuchi and S. Takahashi. 1996. A possible involvement of melanocortin 1-receptor in regulating feather color pigmentation in the chicken. Biochim. Biophys. Acta 1308:164-168. https://doi.org/10.1016/0167-4781(96)00100-5
  21. Guo, X. L., X. L. Li, Y. Li, Z. L. Gu, C. S. Zheng, Z. H. Wei, J. S. Wang, R. Y. Zhou, L. H. Li and H. Q. Zheng. 2010. Genetic variation of chicken MC1R gene in different plumage colour populations. Br. Poult. Sci. 51:734-739. https://doi.org/10.1080/00071668.2010.518408
  22. Heo, K. N., H. J. Choo, B. Y. Seo, M. N. Park, K. C. Jung, J. Hwangbo, H. K. Kim, E. C. Hong, O. S. Seo and B. S. Kang. 2011. Investigation of TYR and MC1R polymorphisms in Korean chickens and the commercial chickens. CNU J. Agric. Sci. 38:465-471.

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