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Construction and Expression of an Eukaryotic Expression Vector Containing the IER3 Gene

  • Wang, Zhen (Department of Oncology, Affiliated Hospital of Medical College, Qingdao University) ;
  • Yu, Hong-Sheng (Department of Oncology, Affiliated Hospital of Medical College, Qingdao University) ;
  • Yao, Ru-Yong (Central Laboratory, Affiliated Hospital of Medical College, Qingdao University) ;
  • Qiu, Wen-Sheng (Department of Oncology, Affiliated Hospital of Medical College, Qingdao University) ;
  • Yue, Lu (Department of Oncology, Affiliated Hospital of Medical College, Qingdao University) ;
  • Sui, Ai-Hua (Central Laboratory, Affiliated Hospital of Medical College, Qingdao University) ;
  • Liu, Xiang-Ping (Central Laboratory, Affiliated Hospital of Medical College, Qingdao University) ;
  • Liu, Shi-Hai (Central Laboratory, Affiliated Hospital of Medical College, Qingdao University)
  • Published : 2013.01.31

Abstract

Background: More and more research indicate that the immediately early response gene 3 (IER3) is involved inmany biological provesses, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesis and tumour progression. Methods: Here we describe the construction of an eukaryotic expression vector containing IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Western-blotting. Results: Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hours post-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in the transfected cells compared with controls. Conclusion: The recombinate plasmid expression vector was constructed successfully, which may provide a basis for further exploration of function of IER3 in lung cancer.

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