GSTT1 Null Genotype Distribution in the Kumaun Region of Northern India

  • Bag, Arundhati (Institute of Allied Health (Paramedical) Services, Education and Training) ;
  • Upadhyay, Saloni (Blood Bank, Susheela Tiwari Govt. Hospital) ;
  • Jeena, Lalit M. (Institute of Allied Health (Paramedical) Services, Education and Training) ;
  • Pundir, Princi (Department of Biotechnology, Graphic Era University) ;
  • Jyala, Narayan S. (Department of Biochemistry, Govt. Medical College)
  • Published : 2013.01.31


Glutathione S-transferases (GSTs) constitute a multigene family of multifunctional phase II metabolic enzymes. GSTT1, an important member of this group has a wide range of substrates including carcinogens. Total homozygous deletion or null genotype resulting in total lack of enzyme activity exists in populations for this enzyme. Since the null genotype may contribute to lower detoxification of carcinogens, this genotype is expected to increase cancer risk. The frequency of the GSTT1 null genotype is known to vary significantly among populations. However, little is known about its distribution in the hilly Kumaun region of northern India. Therefore, in this study, we determined the prevalence of the GSTT1 null polymorphism in the Kumaun popilation by conducting duplex PCR in 365 voluntary healthy individuals. The GSTT1 null genotype was detected in 18.4% of the individuals. Since GSTs play significant role in xenobiotic metabolism, the present data on GSTT1 genotype distribution should contribute in understanding genetic association with cancer risk in this understudied population.


  1. Di Pietro G, Magno LAV, Rios-Santos F (2010). Glutathione S-transferases: an overview in cancer research. Expert Opin Drug Metab Toxicol, 6, 153-70.
  2. Ekhart C, Rodenhuis S, Smits PHM, Beijnen JH, Huitema ADR (2009). An overview of the relations between polymorphisms in drug metabolizing enzymes and drug transporters and survival after cancer drug treatment. Cancer Treat Rev, 35, 18-31.
  3. Garte S, Gaspari L, Alexandrie A-K, et al (2001). Metabolic gene polymorphism frequencies in control populations. Cancer Epidemiol Biomark Prev, 10, 1230-48.
  4. Hayes JD, Pulford DJ (1995). The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol, 30, 445-600.
  5. Kashyap VK, Ashma R, Gaikwad S, Sarkar BN, Trivedi R (2004). Deciphering diversity in populations of various linguistic and ethnic affiliations of different geographical regions of India: analysis based on 15 microsatellite markers. J Genet, 83, 49-63.
  6. Landi S (2000). Mammalian class theta GST and differential susceptibility to carcinogens: a review. Mutat Res, 463, 247-83.
  7. Meyer DJ, Coles B, Pemble SE, et al (1991). Theta, a new class of glutathione transferases purified from rat and man. Biochem J, 274, 409-14.
  8. Mishra DK, Kumar A, Srivastava DSL, Mittal RD (2004). Allelic variation of GSTT1, GSTM1 and GSTP1 genes in North Indian population. Asian Pac J Cancer Prev, 5, 362-5.
  9. Naveen AT, Adithan C, Padmaja N, et al (2004). Glutathione S-transferase M1 and T1 null genotype distribution in South Indians. Eur J Clin Pharmacol, 60, 403-6.
  10. Roy B, Majumder PP, Dey B, et al (2001). Ethnic differences in distributions of GSTM1 and GSTT1 homozygous "null" genotypes in India. Hum Biol, 73, 443-50.
  11. Sambrook J, Fritsch E, Maniatis T (1989). Molecular cloning- a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY
  12. Spurdle AB, Webb PM, Purdie DM, et al (2001). Polymorphisms at the glutathione S- transferase GSTM1, GSTT1 and GSTP1 loci: risk of ovarian cancer by histological subtype. Carcinogenesis, 22, 67-72.
  13. Thoudam RD, Yadav DS, Mishra AK, et al (2010). Distribution of glutathione S- transferase T1 and M1 genes polymorphism in North East Indians: a potential report. Genet Test Mol Biomarkers, 14, 163-9.
  14. Vettriselvi V, Vijayalaksmi K, Paul SFD, Venkatachalam P (2006). Genetic variation of GSTM1, GSTT1 and GSTP1 genes in a South Indian population. Asian Pac J Cancer Prev, 7, 325-8.

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