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Biological Activity and Improvement Effect on Irritable Bowel Syndrome of Wax Gourd Extract and Probiotic Lactic Acid Bacteria

동아 추출물과 프로바이오틱 유산균의 생리활성 및 과민성대장증후군 개선 효과

Ann, Yong-Geun;Jang, Byeong-Churl;Park, Se-Joon
안용근;장병철;박세준

  • Received : 2013.03.04
  • Accepted : 2013.03.11
  • Published : 2013.03.31

Abstract

Biological activities of wax gourd (Benincase hispida) extract and lactic acid bacteria (Lactobacillus casei and Bifidobacterium bifidum) were investigated in this study. Wax gourd extract reduced the activity of angiotensin-converting enzyme (ACE) by 47.9%, of tyrosinase by 13.2%, and had an anti-oxidant activity of 23.4%. Oral administration of wax gourd extract for 72 hours improved the symptom of loose bowels for 120 patients with its highest improvement rates within 6 to 12 hours. The improvement rates were standardized by the curative state by 80%. Lactic acid bacteria preparations reduced the activity of ACE by 21.49%. Oral administration of lactic acid bacteria preparations for 72 hours improved the symptom of loose bowels for 108 patients with its highest improvement rates after 24 hours. On the basis of these results, the tablets containing both wax gourd extract and lactic acid bacteria preparations for the improvement of irritable bowel syndromes were developed. The tablets reduced the activity of ACE by 27.1% and exhibited an anti-oxidant activity of 20.3%. Treatment of the tablets at 100 ${\mu}g/m{\ell}$ and 250 ${\mu}g/m{\ell}$ for 24 hours inhibited the growth of A549 human lung cancer cells by 67%, which was much higher than that of each wax gourd extract or lactic acid bacteria. In addition, treatment of the tablets at 100 ${\mu}g/m{\ell}$ for 24 hours reduced the growth of HCT-116 human colon cancer cells by 70%. Oral administration of the tablets to the patients with loose bowels led to higher improvement rates and speed than each wax gourd extract or lactic acid bacteria. Oral administration of the tablets to the patients with irritable bowel syndromes of loose bowels, constipation, or general type for 72 hours improved their symptoms by 100% with the highest improvement rates within 3 to 6 hours. Furthermore, the improvement rates and speed by the tablets was much higher than each wax gourd extract or lactic acid bacteria.

Keywords

wax gourd;probiotics;lactic acid bacteria;IBS;biological activity

References

  1. Eli Lilly & Co. 2009. Isoxazole derivatives for the treatment of irritable bowel syndrome. Korea Patent 896497
  2. Gilliland SE. 1990. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol Rev 87:174-188
  3. Hotta M, Sato Y, Iwata S, Yamashita N, Sunakawa K, Oikawa T, Tanaka R, Watanabe K, Takayama H, Yajima M, Sekiguchi S, Arai S, Sakurai T, Mutai M. 1987. Clinical effects of Bifidobacterium preparations on pediatric intractable diarrhea. Keio J Med 36:298-314 https://doi.org/10.2302/kjm.36.298
  4. KFDA. 2009a. 4.2-1. Disintegration test. pp4. Health Functional Food Code
  5. KFDA. 2009b. 3.2. Dietary fiber. pp. 106-118. Health Functional Food Code
  6. KFDA. 2009b. 3.2-51. Probiotics. pp. 124-126. Health Functional Food Code
  7. KFIA. 2000. General composition. pp. 539-577. Food Code
  8. Lee JK, Kang MG, Lee JS. 2011. Nutritional characteristics and physiological functionality of lipase inhibitor-containing Desmodium oxyphyllum DC. extracts. Korean J Food Nutrition 24:153-158 https://doi.org/10.9799/ksfan.2011.24.2.153
  9. Medical today. 2007. 4.3. Domestic sales stop of celance, a Parkinson's disease drug to follow zelmac
  10. News Letter. 2005.4. Constipation prevention and treatment information
  11. Saavedra J, Abi-Hanna MA, Moore N, Yolken RH. 2004. Longterm consumption of infant formulas containing live probiotic bacteria: tolerance and safety. Am J Clin Nutr 79:261-267
  12. Thibault H, Jacquin A, Goulet O. 2004. Effects of long-term consumption of a fermented infant formula (with Bifidobacterium brevis c50 and Streptococcus thermophilus 065) on acute diarrhea in healthy infants. J Pediatr Gastroenterol Nutr 39:147-152 https://doi.org/10.1097/00005176-200408000-00004
  13. 許浚. 1610. 東醫寶鑑. pp. 1112. 南山堂
  14. Allen SJ, Okoko B, Martinez EG, Gregorio GV, Dans LF. 2004. Probiotics for treating infectious diarrhea. Art No CD003048. Cochrane Database of Systematic Reviews
  15. Ann YG. 2001. Studies on wax gourd - ginseng vinegar - 2. Korean J Food Nutrition 14:239-244
  16. Ann YG. 2002a. Process for preparing ginseng vinegar. Korea Patent 100344949
  17. Ann YG. 2002b. Protease in wax gourd. Korean J Food Nutrition 15:131-136
  18. Ann YG. 2011. Probiotic latic acid bacteria. Korean J Food Nutrition 24:817-832 https://doi.org/10.9799/ksfan.2011.24.4.817
  19. Ann YG, Kim SK, Shin CS, Min JH. 2002. Inhibitory effect of wax gourd on melanin formation and acne-forming bacterial growth. Korean J Food Nutrition 15:137-143
  20. Ann YG, Shin SC, Kim SK, Shin CS. 2000. Studies on wax gourd wine. Korean J Food Nutrition 13:578-584
  21. Astellas Pharma Inc. 2007. Treating agent for diarrhea-predominant irritable bowel syndrome. Korea Patent 746444
  22. Byeon YS, Lee JI. 2003. Stress and constipation in female high school students. J Korean Acad Funda Nurs 10:23-29
  23. Napo Pharmaceuticals Inc. Co. 2008. Method for treatment of constipation-predominant irritable bowel syndrome. Korea Patent 7029447

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