Effects of ethanolic extract of Ulmus davidiana Root on Lipid Metabolism in High-Fat Diet Fed Mice

느릅나무 뿌리 에탄올 추출물이 고지방 식이를 섭취한 마우스의 지질대사에 미치는 영향

  • Um, Min Young (Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Choi, Won Hee (Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Ahn, Jiyun (Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Ha, Tae Youl (Division of Metabolism and Functionality Research, Korea Food Research Institute)
  • 엄민영 (한국식품연구원 대사기능연구본부) ;
  • 최원희 (한국식품연구원 대사기능연구본부) ;
  • 안지윤 (한국식품연구원 대사기능연구본부) ;
  • 하태열 (한국식품연구원 대사기능연구본부)
  • Received : 2012.10.23
  • Accepted : 2013.01.24
  • Published : 2013.03.31


This study was performed to investigate the effects of ethanolic extract of Ulmus davidiana root (UE) on lipid metabolism in mice fed a high-fat diet (HF) for 7 weeks. Forty male ICR mice were randomly divided into four groups; normal diet group (N), high-fat diet group (HF), HF with 0.5% UE (HF-L) and 1% UE (HF-H) group. Body weight, body weight gain, and liver weight in the HF group was significantly higher than in the N group, while those of the HF-L and HF-H group were unchanged. UE improved HF-induced dyslipidemia by reducing serum triglyceride, total cholesterol, and the atherogenic index. There was no difference in serum HDL-cholesterol among experimental groups. However, the HDL-cholesterol/total cholesterol ratio was significantly increased in the HF-L and HF-H group. Histological analysis showed that HF-fed mice developed hepatocellular microvesicular vacuolation as a result of fat accumulation. These changes were attenuated by 1% UE supplementation. In addition, hepatic triglyceride and cholesterol levels in the HF-H group significantly reduced. Taken together, these results demonstrated that lipid levels in the blood and liver were reduced by UE, suggesting that it might be beneficial for the prevention and treatment of hyperlipidemia and fatty liver.


Supported by : 지식경제부


  1. Ahn JJ, Park JH. 2010. Antioxidant activity and protective effect on DNA damage of extracts from Ulmus davidiana var. japonica. J Appl Oriental Med 10:9-16
  2. Bose M, Lambert JD, Ju J, Reuhl KR, Shapses SA, Yang CS. 2008. The major green tea polyphenol, (-)-epigallocatechin-3-gallate, inhibits obesity, metabolic syndrome, and fatty liver disease in high-fat-fed mice. J Nutr 138:1677-1683
  3. Castelli WP, Garrison RJ, Wilson PW, Abbott RD, Kalousdian S, Kannel WB. 1986. Incidence of coronary heart disease and lipoprotein cholesterol levels. The Framingham Study. JAMA 256:2835-2838
  4. Cho YS, Shon MY, Lee MK. 2007. Lipid-lowering action of powder and water extract of mulberry leaves in C57BL/6 mice fed high-fat diet. J Korean Soc Food Sci Nutr 36: 405-410
  5. Choi SY, Lee S, Choi WH, Lee Y, Jo YO, Ha TY. 2010. Isolation and anti-inflammatory activity of bakuchiol from Ulmus davidiana var. japonica. J Med Food 13:1019-1023
  6. Choi WH, Oh YS, Ahn JY, Kim S, Ha TY. 2005. Antioxidative and protective effects of Ulmus davidiana var. japonica extracts on glutamate-induced cytotoxicity in PC12 cells. Korean J Food Sci Technol 37:479-483
  7. Folch J, Less M, Stanley GH. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497-569
  8. Guo Y, Wu G, Su X, Yang H, Zhang J. 2009. Antiobesity action of a daidzein derivative on male obese mice induced by a high-fat diet. Nutr Res 29:656-663
  9. Haglund O, Luostarinen R, Wallin R, Wibell L, Saldeen T. 1991. The effects of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in humans supplemented with vitamin E. J Nutr 121:165-169
  10. Hwang YP, Choi JH, Han EH, Kim HG, Wee JH, Jung KO, Jung KH, Kwon KI, Jeong TC, Chung YC, Jeong HG. 2011. Purple sweet potato anthocyanins attenuate hepatic lipid accumulation through activating adenosine monophosphateactivated protein kinase in human HepG2 cells and obese mice. Nutr Res 31:896-906
  11. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. 1993. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 342:1007-1011
  12. Hong ND, Rho YS, Kim NJ, Kim JS. 1990. A studies on the constituents of Ulmus davidiana. Korean J Pharmacogn 21:201-204
  13. Jeong KY, Kim ML. 2012. Physiological activities of Ulmus davidiana L. extracts. Korean J Food Preserv 19:104-109
  14. Jin UH, Lee DY, Kim DS, Lee IS, Kim CH. 2006. Induction of mitochondria-mediated apoptosis by methanol fraction of Ulmus davidiana Planch (Ulmaceae) in U87 glioblastoma cells. Environ Toxicol Pharmacol 22:136-141
  15. Jun CD, Pae HO, Kim YC, Jeong SJ, Yoo JC, Lee EJ, Choi BM, Chae SW, Park RK, Chung HT. 1998. Inhibition of nitric oxide synthesis by butanol fraction of the methanol extract of Ulmus davidiana in murine macrophages. J Ethnopharmacol 62:129-135
  16. Kim HJ, Chio SH, Chun BG, Kim DH. 2011. The role of adipose tissue vasculature in energy balance. J Korean Soc Pediatr Endocrinol 16:139-156
  17. Krenisky JM, Luo J, Reed MJ, Carney JR. 1999. Isolation and antihyperglycemic activity of bakuchiol from Otholobium pubescens (Fabaceae), a Peruvian medicinal plant used for the treatment of diabetes. Biol Pharm Bull 22:1137-1140
  18. Lee JC, Lee KY, Son YO, Choi KC, Kim J, Truong TT, Jang YS. 2005. Plant-originated glycoprotein, G-120, inhibits the growth of MCF-7 cells and induces their apoptosis. Food Chem Toxicol 43:961-968
  19. Lee SE, Kim YS, Kim JE, Bang JK, Seong NS. 2004. Antioxidant activity of Ulmus davidiana var. japonica N. and Hemipteleae davidii P. Koearn J Medicinal Crop Sci 12:321-327
  20. Moon JH, Sung J, Choi IW, Kim YS. 2010. Anti-obesity and hypolipidemic activity of taro powder in mice fed with high fat and cholesterol diets. Korean J Food Sci Technol 42: 620-626
  21. Ohyama K, Furuta C, Nogusa Y, Nomura K, Miwa T, Suzuki K. 2011. Catechin-rich grape seed extract supplementation attenuates diet-induced obesity in C57BL/6J mice. Ann Nutr Metab 58:250-258
  22. Ryu HJ, Um MY, Ahn J, Jung CH, Huh D, Kin TW, Ha TY. 2011. Anti-obesity effect of Hypsizigus marmoreus in high fat-fed mice. J Korean Soc Food Sci Nutr 40:1708-1714
  23. Statistics Korea. 2012. Annual report on the causes of death statistics.
  24. Yang EJ, Cho YS, Choi MS, Woo MN, Kim MJ, Shon MY, Lee MK. 2009. Effect of young barley leaf on lipid contents and hepatic lipid-regulating enzyme activities in mice fed highfat diet. Korean J Nutr 42:14-22
  25. Yang HH, Son JK, Jung B, Zheng M, Kim JR. 2011. Epifriedelanol from the root bark of Ulmus davidiana inhibits cellular senescence in human primary cells. Planta Med 77:441-449
  26. Yugarani T, Tan BK, Teh M, Das NP. 1992. Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. Lipids 27:181-186

Cited by

  1. Effects of Alginate Oligosaccharide on Lipid Metabolism in Mice Fed a High Cholesterol Diet vol.43, pp.4, 2014,
  2. Effect of the supplementation of Coconopsis lanceolata extract on lipid metabolism amelioration in type 2 diabetes mouse model induced by high fat diet vol.21, pp.1, 2014,