DOI QR코드

DOI QR Code

Effects of Yeast Culture Supplementation on Rice Straw Digestibility and Cellulolytic Bacterial Community in the Rumen

볏짚 조사료에 대한 효모 배양물 첨가가 반추위 소화율 및 섬유소 분해균의 군락 변화에 미치는 영향

  • Sung, Ha Guyn (Dept. of Animal Science & Technology, Sangji University)
  • 성하균 (상지대학교 동물자원과학과)
  • Received : 2013.01.22
  • Accepted : 2013.02.25
  • Published : 2013.02.28

Abstract

In vitro and in situ incubation studies were conducted to determine effects of yeast culture supplements (Saccharomyces cerevisiae) on cellulolytic bacterial function and fiber digestion in rice straw. In vitro dry matter digestibility of rice straw gradually increased according to supplemental levels of yeast culture (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0%). Digestibility of rice straw started to increase apparently when yeast culture was added more than 0.6% level (p<0.05). Also, we reconfirmed that in vitro dry matter digestibility was significantly increased by 0.6% of yeast culture addition in 4% NaOH treated and non-treated rice straws (p<0.05). When in situ dry matter digestibility was tested in Korean native goats fed basal diet or experimental diet which contained 1.0% of yeast culture, the yeast culture feeding improved in situ dry matter digestibility in both 4% NaOH treated and non-treated rice straws (p<0.05). In case of real-time PCR monitoring cellulolytic bacterial function, the bacterial population attached on rice straw showed the increasing trends with higher level of yeast culture spraying on rice straw. F. succinogenes and R. flavefaciens were significantly increased in accordance to spraying levels of yeast culture (0.0, 0.1 and 0.3%) at both 12 and 24 hrs of in situ incubation (p<0.05). R. albus was significantly higher population in yeast culture spraying than non-soraying at 12 hrs of in situ incubation (p<0.05). These bacterial populations were showed the increasing trends with digestibility enhancement of rice straw according to the higher levels of yeast culture supplement. Overall, these results clearly suggest that the presence of yeast culture result in noticeable increase of rice straw digestion, which is modulated via good effect on cellulolytic bacterial attachment to fiber substrates.

Keywords

Rice straw digestion;Saccharomyces cerevisiae;F. succinogenes;R. flavefaciens;R. albus

References

  1. Adams, D. C., Galycan, M. L., Kiesling, H. E., Wallace, J. D. and Finkner, M. D. 1981. Influence of viable yeast culture, sodium bicarbonate and menensin on liquid dilution rate, rumen fermentation and feedlot performance of growing steers and digestibility in lambs. J. Anim. Sci. 53:780-782. https://doi.org/10.2527/jas1981.533780x
  2. Chaucheyras-Durand, F. and Fonty, G. 2001. Establishment of cellulolytic bacteria and development of fermentative activities in the rumen of gnotobiotically-reared lamb receiving the microbial additive Saccharomyces cerevisiae Cncm I-1077. Reprod. Nutr. Dev. 41:57-68. https://doi.org/10.1051/rnd:2001112
  3. Chaucheyras-Durand, F. and Fonty, G. 2002. Influence of a probiotic yeast (Sccharomyces cerevisiae CNCM I-1077) on microbial colonization and fermentation in the rumen of newborn lambs. Microb. Ecol. Health Dis. 14:30-36. https://doi.org/10.1080/089106002760002739
  4. Chevaux, E. and Fabre, M. M. 2007. Probiotic yeast in small ruminants. Feed Mix. 15:2809-2016.
  5. Dann, H. M., Drackely, J. K., McCoy, G. C., Hutjens, M. F. and Garrett, J. E. 2000. Effests of a yeast culture (Saccharomyces cerevisiae) on prepartum intake and postpartum intake and milk production of Jersey cows. J. Dairy Sci. 83:123-127. https://doi.org/10.3168/jds.S0022-0302(00)74863-6
  6. Dawson, K. A. 1987. Mode of action of yeast cultures in rumen-natural fermentation modifiers. Proceedings, Alltech's Third Annual Symposium. Biotechnology in the Feed Industry. Lexington. USA.
  7. Dawson, K. A. 1992. Current and future role of yeast culture in animal production: A review of research over the last six years. In: Supplement to the Proceedings of Alltech's 8th Annual Symposium. Alltech Technical Publication, Nicholasville, KY, USA, pp. 45-46.
  8. Dawson, K. A. and Hopkins, D. M. 1991. Differential effects of live yeast on the cellulolytic activities of anaerobic ruminal bacteria. J. Anim. Sci. 69(Suppl. 1):531. https://doi.org/10.2527/1991.692531x
  9. Dawson, K. A. and Tricarico, J. 2002. The evolution of yeast cultures-20 years of research. In: Navigating from Niche Markets to Mainstream. Proceeding of Alltech's European, Middle Eastern and African Lecture Tour, pp. 26-43.
  10. Dawson, K. A., Neuma, K. E. and Boling, J. A. 1990. Effects of microbial supplements containing yeast and lactobacilli on roughage-fed ruminal microbial activities. J. Anim. Sci. 68:3392-3398. https://doi.org/10.2527/1990.68103392x
  11. Denev, S. A., Peeva, Tz., Radulova, P., Stancheva, N., Staykova, G., Beev, G., Todorova, P. and Tchobanova, S. 2007. Yeast culture in ruminant nutrition. Bulgarian J. of Agricultureal Sci. 13:357-374.
  12. Devandra, C. 1982. Perspectives in the utilization of untreated rice straw by ruminants in Asia. In: The utilization of fibrous agricultural residue as animal feeds. ed. P. T. Doyle. School of Agric. and Foresty. Univ. of Melvourne. Aust.
  13. Duncan, D. B. 1955. Multiple range and multiple F test. Biometrics. 11:1-42. https://doi.org/10.2307/3001478
  14. Fallon, R. J. and Earley, B. 2004. Effects of Yea-Sacc$^{(R)}$ 1026 inclusion on the performance of finishing bulls offered an all concentrate diet. Proceeding of the 20th Annual Symposium "Nutritional Biotechnology in the Feed and Food Industries" (Suppl. 1), Lexington, KY. USA, May 24-26, pp. 75.
  15. Girard, I. D. 1997. Characterization of stimulatory activities of Saccharomyces cerevisiae 1026 on the growth and metabolism of ruminal bacteria. In: Alltech's 13th Annual symposium Biotechnology in the Feed Industry, Lexington, kentucky, USA. pp. 45-47.
  16. Gunter, K. D. 1989. Yeast cultures success under German dairy conditions. In: Biotechnology in the Feed Industry. Vol. 5, Alltech's Technical Publications. Nicholasville, KY. USA. pp. 38.
  17. Harrison, G. A., Henken, R. W., Dawson, K. A., Harmon, R. J. and Barker, K. B. 1988. Influence of addition of yeast culture supplement to diets of lactating cows on ruminal fermentation and microbial populations. J. Dairy Sci. 71:2967-2979. https://doi.org/10.3168/jds.S0022-0302(88)79894-X
  18. Hoyos, G., Garica, L. and Medina, F. 1987. Effects of feeding viable microbial feed additives on performance of lactating cows in a large dairy herd. J. Dairy Sci. 70(Suppl.):217(Abstr.).
  19. Jankson, M. G. 1977. The alkali treatment of straw. Anim. Feed Sci. Technol. 2:105-116. https://doi.org/10.1016/0377-8401(77)90013-X
  20. Jouany, J. P. 1994. Methods of manipulating the microbial metabolism in the rumen. Ann. Zootech. 43:49-62. https://doi.org/10.1051/animres:19940104
  21. Jouany, J. P., Michalet-Doreau, B. and Doreau, M. 2000. Manipulation of the rumen ecosystem to support highperformance beef cattle-review. Asian Aus. J. Anim. Sci. 13:96-114. https://doi.org/10.5713/ajas.2000.96
  22. Jounany, J. P. 2001. 20 years of research and now more relevant than ever- the coming of age of yeast cultures in ruminant diets. In: Respomding to a Changing Agricultural Landscape. Alltech's European, Middle Eastern an African Lecture Tour. pp. 44-69.
  23. Koike, S. and Kobayashi, Y. 2001. Development and use of competitive PCR asays for the rumen cellulolytic bacteria : Fibrobacter succinogens, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiol. Lett. 204. 361-366. https://doi.org/10.1111/j.1574-6968.2001.tb10911.x
  24. Koike, S., Pan, J., Kobayashi, Y. and Tanaka, K. 2003. Kinestics of in sacco fiber-attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR. J. Dairy Sci. 86:1429-1435. https://doi.org/10.3168/jds.S0022-0302(03)73726-6
  25. Kumar, U., Sareen, V. K. and Singh, S. 1997. Effect of yeast culture supplement on ruminal microbial population and metabolism in buffalo calves fed a high roughage diet. J. Dci. Food Agric. 73:231-236. https://doi.org/10.1002/(SICI)1097-0010(199702)73:2<231::AID-JSFA710>3.0.CO;2-D
  26. Lee, S. S., Pack, N. H., Jung, J. K. and Won, Y. S. 1997. Effects of yeast culture supplements on growth and carcass traits in Korean cattle. Korean J. Anim. Sci. 39:391-400.
  27. Lynch, H. A. and Martin, S. A. 2002. Effects of Saccharomyces cerevisiae culture and Saccharomyces cerevisiae live cells on in vitro mixed ruminal microorganism fermentation. J. Dairy Sci. 85:2009-2014. https://doi.org/10.3168/jds.S0022-0302(02)74277-X
  28. Martin, S. A., Nisbet, D. J. and Dean, R. G. 1989. Influence of a commercial yeast supplement on the in vitro ruminal fermentation. Nutr. Rep. Int. 40:395-417.
  29. Mathieu, F., Jouany, J. P., Senaud, J., Bohatier, J., Bertin, G. and Mercier, M. 1996. The effect of Saccharomyces cerevisiae and Aspergillus oryzae on fermentation in the rumen of faunated and defaunated sheep. protozoal and probiotic interactions. Reprod. Nutr. Dev. 36:271-287. https://doi.org/10.1051/rnd:19960305
  30. McAllister, T. A., Bae, H. D., Jones, G. A. and Cheng, K. J. 1994. Microbial attachment and feed digestion in the rumen. J. Anim. Sci. 72:3004-3018. https://doi.org/10.2527/1994.72113004x
  31. McDougall, E. I. 1948. Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochem. J. 43:99-109. https://doi.org/10.1042/bj0430099
  32. Mehrez, A. A. and Orskov, E. I. 1977. A study of the artificial bac technique for determining the digestibility of feeds in the rumen. J. Agr. Sci. 88:645-652. https://doi.org/10.1017/S0021859600037321
  33. Miller-Webster, T., Hoover, W. H., Holt, M. and Nocek, J. E. 2002. Influence of yeast culture on ruminal microbial metabolism in continuous culture. J. Dairy Sci. 85:2009-2014. https://doi.org/10.3168/jds.S0022-0302(02)74277-X
  34. Monsoni, P., Chaucheyras, F., Bera-Maillet, C. and Forano, E. 2007. Quantification by real-time PCR of cellulolytic bacteria in the rumen of sheep after supplementation of a forage diet with readily fermentable carbohtdrates: effects of a yeast additive. J. of Appl. Microbiol. 103:2676-2685. https://doi.org/10.1111/j.1365-2672.2007.03517.x
  35. Mpofu, I. D. T. and Ndlovu, L. R. 1994. The potential of yeast and natural fungi for enhancing fibre digestibility of forage and roughages. Anim. Feed Sci. and Tech. 48:39-47. https://doi.org/10.1016/0377-8401(94)90110-4
  36. Newbold, C. J., Wallace, R. J. and Mclntosh, F. M. 1996. Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants. Brit. J. Nutr., 76:249-256. https://doi.org/10.1079/BJN19960029
  37. Newbold, C. J., Wallace, R. J., Chen, X. B. and Mclntosh, F. M. 1995. Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep. J. Anim. Sci. 73:1811-1818. https://doi.org/10.2527/1995.7361811x
  38. Padel, A. M. A. 2007. Effects of supplemental yeast (Saccharomyces cerevisiae) culture on NDF digestibility and rumen fermentation of foragr sorghum hay in Nubian goat's kids. J. Agric. and Biol. Sci. 3:133-137.
  39. Palata, F. P., Mendoza, G. D., Barcena-Gama, J. R. and Gonzalez, S. M. 1994. effect of a yeast culture (Saccharomyces cerevisiae) on neutral detergent fiber digestion in steers fed oat straw based diets. Anim. Feed Sci. Tech. 49:203-210. https://doi.org/10.1016/0377-8401(94)90046-9
  40. Park, H. S., Yoo, Y. H., Jeon, B. S. and Cha, J. O. 1996. Effect of feeding viable yeast and lactobacillus on milk yield and milk fat content. Korean J. Anim. Sci. 38:77-84.
  41. Paryad, A. and Rashidi, M. 2009. Effect of yeast (Saccharomyces cerevisiae) on apparent digestibility and nitrogen retention of tomato pomace in sheep. Pakistan J. of Nutrition. 8:273-278. https://doi.org/10.3923/pjn.2009.273.278
  42. Phillips, W. A. and VonTungeln, D. L. 1985. The effect of yeast culture on the post stressperformance of feeder calves. Nutr. Rep. Int. 32:287-296.
  43. Purdy, K. J., Embley, T. M., Takii, S. and Nedwell, D. B. 1996. Rapid extraction of DNA and RNA from sediments by novel hydroxyapatite spin-colum method. Appl. Environ. Microbial. 62:3905-3970.
  44. Rose, A. H. 1980. Rent research on industrially important strains of Saccharomyces cerevisae. In: Skinner, F.A., Passmores, S.M. and Danenport, R.R. (ed.) Biology and Activities of Yeasts. The Society for Applied Bacteriology Symposium Series. 9:103. Academic Press. London. UK.
  45. SAS. 1996. SAS/STAT software$^{(R)}$ for PC. SAS Institute Inc., Cary, NC, USA.
  46. Smith, W. A., Harris, B., Van Horn, H. H. and Wilcox, C. J. 1993. Effects of forage type on production of dairy cows supplemented with whole cottonseed, tallow and yeast. J. Daily Sci. 76:205-213. https://doi.org/10.3168/jds.S0022-0302(93)77339-7
  47. Sung, H. G., Kobayashi, Y., Chang, J., Ha, A., Hwang, I. H. and Ha, J. K. 2009. Low ruminal pH reduces dietary fiber digestion via reduced microbial attachment. Asian-Aust. J. Anim, Sci. 20:200-207.
  48. Sung, H. G., Lee, J. K., Seo, S., Lim, D. C. and Kim, J. D. 2010. Mold growth and mycotoxin contamination of forages. J. Kor. Grassl. Forage Sci. 30:77-88. https://doi.org/10.5333/KGFS.2010.30.1.077
  49. Tajima, K., Aminov, R. I., Nagamine, T., Matsui, H., Nakamura, M. and Benno, Y. 2001. Diet-Dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Appl. Environ. Microb. 2766-2744.
  50. Van Keuren, R. W. and Heineman, W. W. 1962. Study of a nylon bag technique for in vitro estimation of forage disappearance rate. J. Anim. Sci. 21:340-345. https://doi.org/10.2527/jas1962.212340x
  51. Wallace, R. J. 1996. The mode of action of yeast culture in modifying rumen fermentation. In: Proceedings of Alltech's 12th Annual Symposium on Biotechnology in the Feed Industry. Nottingham University Press. Loughborough, Leics. UK. pp. 332-338.
  52. Weidmeier, R. D. and Arambel., M. J. 1985. Effects of supplemental Saccharomyces cerevisiae and / or Aspergillus oryzae on rumen fermentation. Proceedings, Conference on Rumen Function, March 23. Chicago. IL. USA.
  53. Weidmeier, R. D., Arambel, M. J. and Walters, J. L. 1987. Effect of yeast culture and Aspergillus oryzae fermentation extract on ruminal characteristics and nutrient digestibility. J. Dairy Sci. 70:2063-2079. https://doi.org/10.3168/jds.S0022-0302(87)80254-0
  54. Williams, P. E. V. and Newbold, C. J. 1990. The effects of novel microorganisms on rumen fermentation and ruminant productivity. In: Recent Advances in Animal Nutrrition 1990 (Ed. by Cole, D.J.A. and W. Haresign). Butterworths, London.
  55. Williams, P. E. V., Tait, C. A. G. Innes, G. M. and Newbold. C. J. 1991. Effects of the inclusion of the yeast culture (Saccharomyces cerevisiae plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers. J. Anim. Sci. 69:3016-3025. https://doi.org/10.2527/1991.6973016x
  56. Dawson, K. A. 1992. Current and future role of yeast culture in animal production: A review of research over the last six years. In: Supplement to the Proceedings of Alltech's 8th Annual Symposium. Alltech Technical Publication, Nicholasville, KY, USA, pp45-56.

Cited by

  1. Study on Roughage Degradation and Adhesion of Rumen Fibrolytic Bacteria by Real-Time PCR vol.34, pp.1, 2014, https://doi.org/10.5333/KGFS.2014.34.1.60