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Effects of Mustard Seeds and Powder on In vitro Ruminal Fermentation Characteristics and Methane Production

겨자종자와 겨자분의 첨가가 반추위 발효성상과 메탄생성에 미치는 영향

  • 이강연 (농촌진흥청 국립축산과학원) ;
  • 김경훈 (농촌진흥청 국립축산과학원) ;
  • 백열창 (농촌진흥청 국립축산과학원) ;
  • 옥지운 (농촌진흥청 국립축산과학원) ;
  • 설용주 (농촌진흥청 국립축산과학원) ;
  • 한기준 (농촌진흥청 국립축산과학원) ;
  • 박근규 (건국대학교 동물자원센터) ;
  • 류호태 (자연과기술(주)) ;
  • 이상석 (순천대학교 동물자원과학과) ;
  • 전체옥 (중앙대학교 자연과학대학) ;
  • 오영균 (농촌진흥청 국립축산과학원)
  • Received : 2012.04.30
  • Accepted : 2013.02.26
  • Published : 2013.02.28

Abstract

The purpose of this study was to investigate the effects of mustard, which contains allyl isothiocyanate, on ruminal fermentation and methane emission in vitro. To this end, diluted ruminal fluid(30ml) was incubated anaerobically at $39^{\circ}C$ or 6, 12, and 24 h with or without seeds or powdered mustard. Either mustard seed or powdered mustard was weighed and serially (0, 3.33, 5.00, 6.67, and 8.33 g/L) mixed with ruminal fluid. Ammonia-N was increased (P < 0.05) by mustard treatment in a dose dependent manner. Regardless of concentration or form, mustard increased (P < 0.05) total VFA content but decreased (P < 0.01) pH compared to control group. Molar proportion of acetate (A) was decreased (P < 0.05) whereas propionate (P) was increased (P < 0.05) by mustard treatment, thereby A:P ratio was decreased (P < 0.05) compared to control group. Total gas production was increased (P < 0.01) in a linear manner by mustard treatment compared to control group. There was no effect of mustard powder, except 8.33 g/L level at 6 h, on methane emission. However, at 24 h, methane emission was reduced (P < 0.05) by 4.77% and 11.54% with 6.67 g/L and 8.33 g/L of mustard seeds supplementation, respectively. Altogether, these results suggest that mustard seeds containing allyl isothiocyanate may reduce methane production without disturbing ruminal fermentation.

Keywords

Mustard;Methane;Ruminal fermentation

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