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Effects of Kohlrabi (Brassica oleracea var. Gongylodes) on Proliferation and Differentiation of Pig Preadipocytes and 3T3-L1 Cells

콜라비가 돼지 지방전구세포와 3T3-L1 cell의 증식과 분화에 미치는 영향

  • Song, Mi-Yeon (Department of Animal Science, Chungbuk National University) ;
  • Lee, Jae-Joon (Department of Food & Nutrition, Chosun University) ;
  • Cha, Seon-Sook (Department of Food & Nutrition, Chosun University) ;
  • Chung, Chung-Soo (Department of Animal Science, Chungbuk National University)
  • 송미연 (충북대학교 농업생명환경대학 축산학과) ;
  • 이재준 (조선대학교 자연과학대학 식품영양학과) ;
  • 차선숙 (조선대학교 자연과학대학 식품영양학과) ;
  • 정정수 (충북대학교 농업생명환경대학 축산학과)
  • Received : 2013.01.22
  • Accepted : 2013.02.20
  • Published : 2013.02.28

Abstract

The current study was carried out to determine the effects of Kohlrabi (Brassica oleracea var. gongylodes) on proliferation and differentiation of pig preadipocytes and $_3T_3-L_1$ cells. Pig preadipocytes were isolated from the backfat of the new-born pigs. Twenty-four hours after seeding, the cells were washed with DMEM/F-12 (designated day 0). To measure the cell proliferation, the cells were treated with 25 ng/ml and 100 ng/ml ethanol extracts of Kohlrabi (peel and flesh) for two days (day 0 ~ 2). To measure differentiation, the cells were treated with Kohlrabi for two days (day 0 ~ 2) and cell differentiation was measured on day 6. Twenty-five ng/ml and 100 ng/ml of Kohlrabi peel decreased proliferation of pig preadipocytes by 4.59% and 17.7%, respectively, compared with the control and Kohlrabi flesh by 11.4% and 19.2%, respectively. However, Kohlrabi did not inhibit cell differentiation. To measure the effects of Kohlrabi on proliferation and differentiation of $_3T_3-L_1$ cells, the cells were treated with Kohlrabi for two days in culture, like pig preadipocytes. Kohlrabi (both peel and flesh) did not show any effects on cell proliferation and differentiation. In summary, the results of the current study showed that Kohlrabi decreased proliferation of pig preadipocytes, but no inhibitory effects on differentiation of the cells. Kohlrabi had no effects on proliferation and differentiation of $_3T_3-L_1$ cells.

Keywords

Kohlrabi;Pig preadipocytes;$_3T_3-L_1$ cells;Proliferation;Differentiation

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