Genetic Diversity of Taenia asiatica from Thailand and Other Geographical Locations as Revealed by Cytochrome c Oxidase Subunit 1 Sequences

  • Anantaphruti, Malinee Thairungroj (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Thaenkham, Urusa (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Watthanakulpanich, Dorn (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Phuphisut, Orawan (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Maipanich, Wanna (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Yoonuan, Tippayarat (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Nuamtanong, Supaporn (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Pubampen, Somjit (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University) ;
  • Sanguankiat, Surapol (Department of Helminthology, Faculty of Tropical Medicine, Mahidol University)
  • Received : 2012.03.26
  • Accepted : 2012.12.27
  • Published : 2013.03.15


Twelve 924 bp cytochrome c oxidase subunit 1 (cox1) mitochondrial DNA sequences from Taenia asiatica isolates from Thailand were aligned and compared with multiple sequence isolates from Thailand and 6 other countries from the GenBank database. The genetic divergence of T. asiatica was also compared with Taenia saginata database sequences from 6 different countries in Asia, including Thailand, and 3 countries from other continents. The results showed that there were minor genetic variations within T. asiatica species, while high intraspecies variation was found in T. saginata. There were only 2 haplotypes and 1 polymorphic site found in T. asiatica, but 8 haplotypes and 9 polymorphic sites in T.saginata. Haplotype diversity was very low, 0.067, in T. asiatica and high, 0.700, in T. saginata. The very low genetic diversity suggested that T. asiatica may be at a risk due to the loss of potential adaptive alleles, resulting in reduced viability and decreased responses to environmental changes, which may endanger the species.


  1. Fan PC. Taiwan Taenia and taeniasis. Parasitol Today 1988; 4: 86-88.
  2. Eom KS, Jeon HK, Rim HJ. Geographical distribution of Taenia asiatica and related species. Korean J Parasitol 2009; 47: S115-S124.
  3. Eom KS, Rim HJ. Morphologic descriptions of Taenia asiatica sp. n. Korean J Parasitol 1993; 31: 1-6.
  4. Fan PC, Lin CY, Chen CC, Chung WC. Morphologic description of Taenia saginata asiatica (Cyclophyllidea: Taeniidae) from man in Asia. J Helminthol 1995; 69: 299-303.
  5. Eom KS. What is Asian Taenia? Parasitol Int 2006; 55: S137-S141.
  6. Zarlenga DS, McManus DP, Fan PC, Cross JH. Characterization and detection of a newly described Asian taeniid using cloned ribosomal DNA fragments and sequence amplification by the polymerase chain reaction. Exp Parasitol 1991; 72: 174-183.
  7. Bowles J, McManus DP. Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. Am J Trop Med Hyg 1994; 50: 33-44.
  8. Jeon HK, Eom KS. Taenia asiatica and Taenia saginata: Genetic divergence estimated from their mitochondrial genomes. Exp Parasitol 2006; 113: 58-61.
  9. Hoberg EP, Alkire NL, Queiroz AD, Jones A. Out of Africa: origins of the Taenia tapeworms in humans. Proc R Soc Lond 2001; 268: 781-787.
  10. Eom KS, Rim HJ. Natural infection of Asian Taenia saginata metacestodes in the livers of Korean domestic pigs. Korean J Parasitol 1992; 30: 15-20.
  11. Eom KS, Rim HJ, Geerts S. Experimental infection of pigs and cattle with eggs of Asian Taenia saginata with special reference to its extrahepatic viscerotropism. Korean J Parasitol 1992; 30: 269-275.
  12. Zhang L, Tao H, Zhang B, Wang H, Wang Y, Li Z, Yang J, Yang B, Li Y, Pang Y, Zhang H, Li Y, Wu Y. First discovery of Taenia saginata asiatica infection in Yunnan province. Chinese J Parasitol Parasit Dis 1999; 17: 95-96 (in Chinese).
  13. De NV, Hoa LT, Doanh NQ, Ngoc NB, Cong LD. Report on a new species of Taenia (Taenia asiatica) in Hanoi, Vietnam. J Malaria Parasit Dis Control 2001; 3: 80-85.
  14. Wandra T, Depary AA, Sutisna P, Margono SS, Suroso T, Okamoto M, Craig PS, Ito A. Taeniasis and cysticercosis in Bali and North Sumatra, Indonesia. Parasitol Int 2006; 55: S155- S160.
  15. Anantaphruti MT, Yamasaki H, Nakao M, Waikagul J, Watthanakulpanich D, Nuamtanong S, Maipanich W, Pubampen S, Sanguankiat S, Muennoo C, Nakaya K, Sato MO, Sako Y, Okamoto M, Ito A. Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand. Emerg Infect Dis 2007; 13: 1413-1416.
  16. Markell EK, Voge M, John DT. Medical Parasitology, 7th ed. Philadelphia, USA. WB Saunders Company. 1992, p 242-244.
  17. Anantaphruti MT, Okamoto M, Yoonuan T, Saguankiat S, Kusolsuk T, Sato M, Sato MO, Sako Y, Waikagul J, Ito A. Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand. Parasitol Int 2010; 59: 326-330.
  18. Anantaphruti MT. Current status of taeniasis in Thailand. Korean J Parasitol 2013; 51: 37-42.
  19. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25: 4876-4885.
  20. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetic analysis using maximum likelihood, evolutionary distance, maximum parsimony methods. Mol Biol Evol 2011; 28: 2731-2739.
  21. Rozas J, Sanchez-DelBarrio JC, Messeguer X, Rozas R. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 2003; 19: 2496-2497.
  22. Matocq MD, Villablanca FX. Low genetic diversity in an endangered species: recent or historic pattern? Biol Conserv 2001; 98: 61-68.