Effect of Temperature on Survival of Escherichia coli O157:H7 and Listeria monocytogenes in Livestock Manure Compost

  • Jung, Kyu-Seok (National Academy of Agricultural Science) ;
  • Heu, Sung-Gi (National Academy of Agricultural Science) ;
  • Roh, Eun-Jung (National Academy of Agricultural Science) ;
  • Kim, Min-Ha (National Academy of Agricultural Science) ;
  • Gil, Hyun-Ji (National Academy of Agricultural Science) ;
  • Choi, Na-Young (National Academy of Agricultural Science) ;
  • Lee, Dong-Hwan (National Academy of Agricultural Science) ;
  • Lim, Jeong-A (National Academy of Agricultural Science) ;
  • Ryu, Jae-Gee (National Academy of Agricultural Science) ;
  • Kim, Kye-Hoon (Dept. of Environmental Horticulture, The University of Seoul)
  • Received : 2013.09.25
  • Accepted : 2013.12.09
  • Published : 2013.12.31


Animal manure compost is a commonly used fertilizer in organic vegetable and fruit production in Korea. However, livestock manure compost produced from animal feces can contain a lot of the non-pathogenic and pathogenic bacteria. Of particular concern are bacteria causing human food-borne illness such as Escherichia coli O157:H7 and Listeria monocytogenes. The objective of this study was to investigate effect of temperature on survival of E. coli O157:H7 and L. monocytogenes in livestock manure compost. Commercial livestock manure compost (manure 60%, sawdust 40%) was inoculated with E. coli O157:H7 and L. monocytogenes. Compost was incubated at four different temperatures (10, 25, 35, and $55^{\circ}C$) for 20 weeks. Samples were taken every week during incubation depending on the given conditions. E. coli O157:H7 persisted for up to 1 day in livestock manure compost at $55^{\circ}C$, over 140 days at $10^{\circ}C$, 140 days at $25^{\circ}C$, and 120 days at $35^{\circ}C$, respectively. L. monocytogenes persisted for up to 1 day in livestock manure compost at $55^{\circ}C$ and 140 days at $10^{\circ}C$, 70 days at $25^{\circ}C$, and 40 days at $35^{\circ}C$, respectively. The results indicated that E. coli O157:H7 and L. monocytogenes persisted longer under low temperature condition. E. coli O157:H7 survived longer than L. monocytogenes at three different temperatures (10, 25, and $35^{\circ}C$). The results are being used to develop guidelines on the management of manure to reduce the risks of E. coli O157:H7 and L. monocytogenes transmission to foods produced in the presence of animal waste.


Livestock manure compost;Escherichia coli O157:H7;Listeria monocytogenes;Survival


Supported by : Rural Development Administration,


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