DOI QR코드

DOI QR Code

Solubilization of Inorganic Phosphates and Plant Growth Promotion by Pantoea Strains

  • Walpola, Buddhi Charana (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2013.07.17
  • Accepted : 2013.11.20
  • Published : 2013.12.31

Abstract

Two phosphate solubilizing Pantoea strains (P. agglomerans and P. rodasii) were employed in elucidating their phosphate solubilizing potential under different carbon and nitrogen sources, pH, temperature and salt conditions. Plant growth promoting characteristics such as ACC deaminase activity, indole acetic acid (IAA), HCN, ammonia, and siderophore production of the two strains were assessed in vitro. Potential applicability of the strains as bio-inoculants was also evaluated in pot experiments conducted under green house conditions. Phosphate solubilization measured as the amount of phosphorous released into the medium was recorded as 810 and $788{\mu}g\;ml^{-1}$ respectively by P. agglomerans and P. rodasii. Glucose at the rate of 2% was found be the best carbon source, while $(NH_4)_2SO_4$ was the best nitrogen source for both strains. Despite a slight decrease in phosphate solubilization observed at higher temperature, pH and salt concentrations, both strains could withstand against a range of temperature ($30-35^{\circ}C$), pH (7-9) and the presence of NaCl (up to 5%) without much compromising the phosphate solubilization. Different plant growth promoting traits (ACC deaminase activity, IAA, HCN, ammonia, and siderophore production) of the strains and their ability to promote the growth of green gram seedlings indicate that both strains possess high potential to be used as bio-inoculants.

Keywords

Pantoea agglomerans;Pantoea rodasii;phosphate solubilization

Acknowledgement

Supported by : National Institute of Horticultural & Herbal Science,

References

  1. Cappucino, J.C. and N. Sherman. 1992. Microbiolgy: A laboratory manual. Benjamin/Cummings Publishing Company, New York, pp. 125-179.
  2. Cimmino, A., A. Andolfi, G. Marchi, G. Surico, and A. Evindente. 2006. Phytohormone production by strain Pantoea agglomerans from knot on olive plants caused by Pseu-domonas savastanoi pv.savastanoi. Phytopathol. Mediterr. 45:247-252.
  3. Collavino, M.M., P.A. Sansberro, L.A. Mroginski, and O.M. Aguilar. 2010. Comparison of in vitro solubilization activity of diverse phosphate-solubilizing bacteria native to acid soil and their ability to promote Phaseolus vulgaris growth. Biol. Fert. Soils. 46:727-738. https://doi.org/10.1007/s00374-010-0480-x
  4. Costa, E., J.Usall, N.Teixido, J. Delgado, and I. Vinas. 2002. Water activity, temperature, and pH effects on growth of the biocontrol agent Pantoea agglomerans CPA-2. Can. J. Microbiol. 48:987-992.
  5. Dastager, S.G., C.K. Deepa1, S.C. Puneet, C.S. Nautiyal, and A. Pandey. 2009. Isolation and characterization of plant growth-promoting strain Pantoea NII-186 from Western Ghat forest soil, India. Lett. Appl. Microbiol. doi:10.1111/j.1472-765X.2009.02616.x https://doi.org/10.1111/j.1472-765X.2009.02616.x
  6. Dave, A. and H.H. Patel. 2003. Impact of different carbon and nitrogen sources on phosphate solubilization by Pseudomonas fluorescens. Indian J. Microbiol. 43:33-36.
  7. Donate-Correa, J., M. Leon-Barrios and R. Perez-Galdona. 2005. Screening for plant growth-promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree-shrub legume endemic to the Canary Islands. Plant Soil. 266:261-272. https://doi.org/10.1007/s11104-005-0754-5
  8. Feng, Y., D. Shen, and W. Song. 2006. Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and effects allocations of host photosynthates. J. App. Microbiol. 100:938-945. https://doi.org/10.1111/j.1365-2672.2006.02843.x
  9. Gutierrez, C.K., G.Y. Matsui, D.E. Lincoln, and C.R. Lovell. 2009. Production of the phytohormone indole-3-acetic acid by the estuarine species of the genus Vibrio. Appl. Environ. Microbiol. 75:2253-2258. https://doi.org/10.1128/AEM.02072-08
  10. Jain, R., J. Saxena, and V. Sharma. 2010. The evaluation of free and encapsulated Aspergillus awamori for phosphate solubilization in fermentation and soil-plant system. Appl. Soil Ecol. 46:90-94. https://doi.org/10.1016/j.apsoil.2010.06.008
  11. Kausar, R. and S.M. Hahzad. 2006. Effect of ACC-deaminase Containing Rhizobacteria on Growth Promotion of Maize under Salinity Stress. J. Agri. Soc. Sci. 2:216-218.
  12. Khalim, K., D.N. Suprapta, and Y. Nitta. 2012. Effect of Pantoea agglomerans on growth promotion and yield of rice. Agric. Sci. Res. J. 2:240-249.
  13. Kumar, S., K. Tamura, I.B. Jakobsen, and M. Nei. 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics. 17:1244-1245. https://doi.org/10.1093/bioinformatics/17.12.1244
  14. Lugo, M.A., M. Ferrero, E. Menoyo, M.C. Estevez, F. Sineriz, and A. Anton. 2008. Arbuscular mycorrhizal fungi and rhizospheric bacteria diversity along a altitudinal gradient in South American Puna grassland. Microb. Ecol. 55:705-713. https://doi.org/10.1007/s00248-007-9313-3
  15. Malboobi, M.A., P. Owlia, M. Behbahani, E. Sarokhani, S. Moradi, B. Yakhchali, A. Deljou, and K.M. Heravi. 2009. Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates. World J. Microbiol. Biotechnol. 25:1471-1477. https://doi.org/10.1007/s11274-009-0037-z
  16. Murphy, J. and J. P. Riley. 1962. A modified single solution method for the determination of phosphate in mineral waters. Anal. Chim. Acta. 27:31-36. https://doi.org/10.1016/S0003-2670(00)88444-5
  17. Nautiyal, C.S. 1999. An efficient microbiological growth medium for screening of phosphate solubilizing microorganisms. FEMS Microbiol. Lett. 170:265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
  18. Nezarat, S. and A. Gholami. 2009. Screening plant growth promoting rhizobacteria for improving seed germination, seedling growth and yield of maize. Pakistan J. Biol. Sci. 12:26-32. https://doi.org/10.3923/pjbs.2009.26.32
  19. Payne, S. M. 1994. Detection, isolation and characterization of siderophores. In:Methods Enzymol. 235:329-344. https://doi.org/10.1016/0076-6879(94)35151-1
  20. Penrose, D.M. and B.R. Glick. 2003. Methods for isolating and characterizing ACC deaminase-containing plant growthpromoting rhizobacteria. Physiol. Plant. 118:10-15. https://doi.org/10.1034/j.1399-3054.2003.00086.x
  21. Rahman, M.M., C.M. Escobedo-Bonilla, M. Corteel, J.J. Dantas-Lima, M. Wille, V. Alday Sanz, M.B. Pensaert, P. Sorgeloos, and H.J. Nauwynck. 2006. Effect of high water temperature ($33^{\circ}C$) on the clinical and virological outcome of experimental infections with white spot syndrome virus (WSSV) in specific pathogen-free (SPF) Litopenaeus vannamei. Aquaculture. 261:842-849. https://doi.org/10.1016/j.aquaculture.2006.09.007
  22. Relwani, L., P. Krishna, and M.S. Reddy. 2008. Effect of carbon and nitrogen sources on phosphate solubilisation by a wild type strain and UV-induced mutants of Aspergillus tubigensis. Curr. Microbiol. 57:401-406. https://doi.org/10.1007/s00284-008-9212-y
  23. Reyes, I., L. Bernier, and H. Antoun. 2002. Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillium rugulosum. Microb. Ecol. 44:39-48. https://doi.org/10.1007/s00248-002-1001-8
  24. Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
  25. Saleena, L.M., S. Rangarajan, and S. Nair. 2002. Diversity of Azospirillum strains isolated from rice plants grown in saline and nonsaline sites of coastal agricultural ecosystem. Microb. Ecol. 44:271-277. https://doi.org/10.1007/s00248-002-2019-7
  26. SAS (1999). SAS/STAT User's Guide Version 8. SAS, Cary, NC.
  27. Scervino, J.M., M.P. Mesa, I.D. Monica, M. Recchi, N.S. Moreno, and A. Godeas. 2010. Soil fungal isolates produce different organic acid patterns involved in phosphate salts solubilization. Biol. Fertil. Soils. 46:755-763. https://doi.org/10.1007/s00374-010-0482-8
  28. Schwyn, R. and J.B. Neilands. 1987. Universal chemical assay for detection and determination of siderophores. Anal. Biochem. 160:47-56. https://doi.org/10.1016/0003-2697(87)90612-9
  29. Silini-Cherif, H., A. Silini, M. Ghoul, and S. Yadav. 2012. Isolation and characterization of plant growth promoting traits of a rhizobacteria: Pantoea agglomerans Ima2. Pakistan J. Biol. Sci. 15:267-276 https://doi.org/10.3923/pjbs.2012.267.276
  30. Son, H.J., G.T. Park, M.S. Cha, and M.S. Heo. 2006. Solubilization of insoluble inorganic phosphates by a novel salt and pH tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Bioresour. Technol. 97:204-210. https://doi.org/10.1016/j.biortech.2005.02.021
  31. Souchie, E.L., O.J. Saggin-Junior, E.M.R. Silva, E.F.C. Campello, R. Azcon and J.M. Barea. 2006. Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi in grass pasture and secondary forest of Paraty, RJ-Brazil. An Acad. Bras. Cienc. 78:1-11. https://doi.org/10.1590/S0001-37652006000100001
  32. Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  33. Viruel, E., M.E. Lucca, and F. Sineriz. 2011. Plant growth promotion traits of phosphobacteria isolated from puna, Argentina. Arch. Microbiol. 193:489-496 https://doi.org/10.1007/s00203-011-0692-y
  34. Viveros, O.M., M.A. Jorquera, D.E. Crowley, G. Gajardo, and M.L. Mora. 2010. Mechanism and practical considerations involved in plant growth promotion by rhizobacteria. J. Soil Sci. plant Nutr. 10:293-319.
  35. Zaidi, A., M.S Khan, and M.D. Amil. 2003. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.). Eur. J. Agron. 19:15-21. https://doi.org/10.1016/S1161-0301(02)00015-1

Cited by

  1. Synergistic effect of co-inoculation with phosphate-solubilizing bacteria vol.43, pp.3, 2016, https://doi.org/10.7744/kjoas.20160043
  2. Application of Immobilization Technology in Solubilization of Rock Phosphate vol.47, pp.4, 2014, https://doi.org/10.7745/KJSSF.2014.47.4.249
  3. Co-culture development and bioformulation efficacy of psychrotrophic PGPRs to promote growth and development of Pea (Pisum sativum) plant pp.1349-8037, 2018, https://doi.org/10.2323/jgam.2018.05.007