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Microbial Community Structure of Paddy Soil Under Long-term Fertilizer Treatment Using Phospholipid Fatty Acid (PLFA) Analysis

  • Daquiado, Aileen Rose (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Tae Young (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Yong Bok (Institute of Agriculture and Life Science, Gyeongsang National University)
  • Received : 2013.10.21
  • Accepted : 2013.11.18
  • Published : 2013.12.31

Abstract

Understanding the microbial community structure of agricultural soils is important for better soil management in order to improve soil quality. Phospholipid fatty acid analysis has been popularly used in determining the microbial community structure in different ecosystems. The microbial community structure of paddy soil under long-term fertilizer treatments was investigated after 45 years using PLFA analysis. Treatments were control (no fertilization, Con), compost (COM), NPK, NPK+compost (NPKC), PK, NK, and NP. Soil chemical properties were mainly affected by the addition of compost and inorganic P fertilizer. Total nitrogen and organic matter contents were significantly higher in treatments with compost while available $P_2O_5$ and exchangeable calcium were significantly higher in treatments with added inorganic P fertilizer. It was found that microbial communities were responsive to the different fertilizer treatments. PLFA results showed that the soils were dominated by gram-negative bacteria, followed by the actinomycetes, then gram-positive bacteria, and fungi. Principal component analysis of the soil chemical properties and PLFA composition proved to be a more reliable tool because it was more responsive to the changes in soil chemical properties.

Keywords

PLFA;Microbial community structure;Long-term fertilization

Acknowledgement

Supported by : Rural Development Administration

References

  1. Acea, M.J. and T. Carballas. 1988. The influence of cattle slurry on soil microbial population and nitrogen cycle microorganisms. Biological Wastes 23:229-241. https://doi.org/10.1016/0269-7483(88)90037-7
  2. Arslan, E.I., E. Obek, S. Kirbag, U. Ipek, and M. Topal. 2008. Determination of the effect of compost on soil microorganisms. International Journal of Science and Technology 3(20):151-159.
  3. Bolton, J., L.F. Elliot, P.R. Papendick, and D.F. Bezdicek. 1985. Soil microbial biomass and selected soil enzyme activities; effect of fertilization and cropping practices. Soil Biology and Biochemistry 17:297-302. https://doi.org/10.1016/0038-0717(85)90064-1
  4. Bouajila, K. and M. Sanaa. 2011. Effects of organic amendments on soil physic-chemical and biological properties. Journal of Materials and Environmental Science 2(S1):485-490.
  5. Cavigelli, M.A., G.P. Robertson, and M.J. Klug. 1995. Fatty acid methyl ester (FAME) profiles as measures of soil microbial community structure. Plant and Soil 170:99-113. https://doi.org/10.1007/BF02183058
  6. Doran, J.W. 1980. Soil microbial and biochemical changes associated with reduced tillage. Soil Science Society of America Journal 44:764-771.
  7. Environmental Literacy Council. Microorganisms. http://www.enviroliteracy.org/article.Php/ 317 .html.
  8. Ferre, C., S. Zechmeister-Boltenstern, R. Comolli, M. Andersson, and G. Seufert. 2012. Soil microbial community structure in a rice paddy field and its relationships to $CH_4\;and\;N_2O$ fluxes. Nutrient Cycling in Agroecosystems 93:35-50. https://doi.org/10.1007/s10705-012-9497-x
  9. Fraser, D.G., J.W. Doran, W.W. Sahs, and G.W. Lesoin. 1988. Soil microbial populations and activities under conventional and organic management. Journal of Environmental Quality 17:585-590.
  10. Frostegard, A., A. Tunlid, and E. Baath. 1991. Microbial biomass measured as a total lipid phosphate in soils of different organic content. Journal of Microbiological Methods 14:151-163. https://doi.org/10.1016/0167-7012(91)90018-L
  11. Frostegard, A., A. Tunlid, and E. Baath. 2010. Use and misuse of PLFA measurements in sois. Soil Biology and Biochemistry 1-5.
  12. Kaur, A., A. Chaudhary, A. Kaur, R. Choudhary, and R. Kaushik. 2005. Phospholipid fatty acid - a bioindicator of environment monitoring and assessment in soil ecosystem. Current Science 89(7):1103-1112.
  13. Lackzo, E., A. Rudaz, and M. Aragno. 1997. Diversity of antropogenically influenced or disturbed soil microbial communities. p. 54-67. In: Insam, H. and A. Rangger (eds.). Microbial Communities - Functional versus Structural Approaches. Springer Verlag, Heidelberg.
  14. Lechevalier, M. P. 1989. Lipids in bacterial taxonomy. p. 455-561. In: O'Leary, W. M. (ed.). Practical Handbook of Microbiology. CRC, Boca Raton, Florida.
  15. Liu, L., P. Gunderson, T. Zhang, and J. Mo. 2011. Effects of phosphorus addition on soil microbial biomass and community composition in three forest types in tropical China. Soil Biology and Biochemistry 44:31-38.
  16. Mullen, M.D., C.G. Melhorn, D.D. Tyler, and B.N. Duck. 1998. Biological and biochemical soil properties in no-till corn with different cover crops. Journal of Soil and Water Conservation 53(3):219-224.
  17. Powlson, D.S., P.C., Brookes, and B.T. Christensen. 1987. Measurement of soil microbial biomass provides an early indication of changes in total soil organic matter due to straw incorporation. Soil Biology and Biochemistry 19:159-164. https://doi.org/10.1016/0038-0717(87)90076-9
  18. Potthoff, M., K.L. Steenwerth, L.E. Jackson, R.E. Drenovsky, K.M. Scow, and R.G. Joergensen. 2006. Soil microbial community composition as affected by restoration practices in California grassland. Soil Biology and Biochemistry 38:1851-1860. https://doi.org/10.1016/j.soilbio.2005.12.009
  19. RDA (Rural Development Administration, Korea) (1988) Method of Soil Chemical Analysis. National Institute of Agricultural Science and Technology, Suwon, Korea.
  20. Ros, M., S. Klammer, B. Knapp, K. Aichberger, and H. Insam. 2006. Long-term effects of compost amendment of soil on functional and structural diversity and microbial activity. Soil Use and Management 22:209-218. https://doi.org/10.1111/j.1475-2743.2006.00027.x
  21. Sarwar, G., H. Schmeisky, N. Hussain, S. Muhammad, M. Ibrahim, and E. Safdar. 2008. Improvement of soil physical and chemical properties with compost application in rice-wheat cropping system. Pakistan Journal of Botany 40(1):275-282.
  22. Steenwerth, K.L., L.E. Jackson, F.J. Calderon, M.R. Stromberg, and K.M. Scow. 2002. Soil microbial community composition and land use history in cultivated and grassland ecosystems of coastal California. Soil Biology and Biochemistry 34:1599-1611. https://doi.org/10.1016/S0038-0717(02)00144-X
  23. Turk, A. and R. Mihelic. 2013. Wheat straw decomposition, N-mineralization and microbial biomass after 5 years of conservation tillage in Gleysol field. Acta Agriculturae Slovenica 101(1):69-75.
  24. Wandruszka, R. V. 2006. Phosphorus retenction in calcareous soils and the effect of organic matter on its mobility. Geochemical Transactions 7:6. https://doi.org/10.1186/1467-4866-7-6
  25. White, D.C., R.J. Bobbie, J.S. Heron, J.D. King, and S.J. Morrison. 1979. Biochemical measurements of microbial mass and activity from environmental samples. p. 69-81. In: Costerton, J.W. and R.R. Colwell (eds). Native Aquatic Bacteria: Enumeration, Activity, and Ecology, ASTM STP 695. American Society for Testing and Materials, Philadelphia, PA.
  26. Widmer, F., A. Fliessbach, E. Laczko, J. Schulze-Aurich, and J. Zeyer. 2001. Assessing soil biological characteristics: a comparison of bulk soil community DNA-, PLFA-, and Biolog $^{TM}$-analyses. Soil Biology and Biochemistry 33:1029-1036. https://doi.org/10.1016/S0038-0717(01)00006-2
  27. Zelles, L. 1999. Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of microbial communities in soil: a review. Biology and Fertility of Soils 29:111-129. https://doi.org/10.1007/s003740050533
  28. Zelles, L. and Q.Y. Bai. 1993. Fractionation of fatty acids derived from soil lipids by solid phase extraction and their quantitative analysis by GC-MS. Soil Biology and Biochemistry 24:317-323.
  29. Zhong, W., T. Gu, W. Wang, B. Zhang, X. Lin, Q. Huang, and W. Shen. 2010. The effects of mineral fertilizer and organic manure on microbial community and diversity. Plant and Soil 326:511-522. https://doi.org/10.1007/s11104-009-9988-y

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