Microbial Community Structure of Paddy Soil Under Long-term Fertilizer Treatment Using Phospholipid Fatty Acid (PLFA) Analysis

  • Daquiado, Aileen Rose (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Tae Young (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Yong Bok (Institute of Agriculture and Life Science, Gyeongsang National University)
  • Received : 2013.10.21
  • Accepted : 2013.11.18
  • Published : 2013.12.31


Understanding the microbial community structure of agricultural soils is important for better soil management in order to improve soil quality. Phospholipid fatty acid analysis has been popularly used in determining the microbial community structure in different ecosystems. The microbial community structure of paddy soil under long-term fertilizer treatments was investigated after 45 years using PLFA analysis. Treatments were control (no fertilization, Con), compost (COM), NPK, NPK+compost (NPKC), PK, NK, and NP. Soil chemical properties were mainly affected by the addition of compost and inorganic P fertilizer. Total nitrogen and organic matter contents were significantly higher in treatments with compost while available $P_2O_5$ and exchangeable calcium were significantly higher in treatments with added inorganic P fertilizer. It was found that microbial communities were responsive to the different fertilizer treatments. PLFA results showed that the soils were dominated by gram-negative bacteria, followed by the actinomycetes, then gram-positive bacteria, and fungi. Principal component analysis of the soil chemical properties and PLFA composition proved to be a more reliable tool because it was more responsive to the changes in soil chemical properties.


PLFA;Microbial community structure;Long-term fertilization


Supported by : Rural Development Administration


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