Effect of Dispersed and Proximate Inoculation Methods of Glomus etunicatum on Root Colonization of Sorghum-Sudangrass Hybrid

  • Lee, Seonmi (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Selvakumar, Gopal (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Krishnamoorthy, Ramasamy (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Kim, Kiyoon (Department of Agricultural Chemistry, Chungbuk National University) ;
  • Choi, Joonho (Department of Food Science and Biotechnology, College of Life Science and Natural Resources, Wonkwang University) ;
  • Sa, Tongmin (Department of Agricultural Chemistry, Chungbuk National University)
  • Received : 2013.08.30
  • Accepted : 2013.09.27
  • Published : 2013.10.31


Information on the effective application method of arbuscular mycorrhizal fungi (AMF) inoculum is still inadequate. This work was performed to assess two AMF inoculation methods (dispersed and proximate) on root colonization of sorghum-sudangrass hybrid (Sorghum bicolor L.). In dispersed inoculation method, spores were inoculated in 2 kg pots of soil in which 5 day-old seedlings were transplanted and maintained for 50 days. In the proximate inoculation method, spores were first introduced in 500 mL pots where seeds were sown. After 10 days, the seedlings with the 500 mL soil were transferred to 2 kg pots without disturbing the contents. After 50 days of growth, root colonization and arbuscule abundance significantly increased (over 100%) in proximate method of inoculation. Moreover, sorghum-sudangrass hybrid had higher shoot growth (182.5 cm) and Glomalin related soil protein (GRSP) production in proximate method. Nutrient accumulation, particularly total nitrogen (82.61 mg $plant^{-1}$), was also found to be higher in proximate method of inoculation. Our results demonstrate that the proximate method of inoculation may improve the early stage mycorrhizal symbiosis and inoculum performance in Saemangeum reclaimed soil.


Grant : Cooperative Research Program for Agriculture Science & Technology Development

Supported by : Rural Development Administration


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