Phosphate Removal Using Recombinant Bacteria with Cytoplasmic Phosphate Binding Protein

Phosphate Binding Protein이 세포질에 발현된 재조합 박테리아를 이용한 인 제거

  • Choi, Suk Soon (Department of Biological and Environmental Engineering, Semyung University) ;
  • Ha, Jeong Hyub (School of Environmental Science and Technology, Pohang University of Science and Technology) ;
  • Cha, Hyung Joon (Department of Chemical Engineering, Pohang University of Science and Technology)
  • 최석순 (세명대학교 바이오환경공학과) ;
  • 하정협 (포항공과대학교 환경공학부) ;
  • 차형준 (포항공과대학교 화학공학과)
  • Published : 2013.10.31

Abstract

In the present work, we constructed a recombinant Escherichia coli with cytoplasmic-expressed phosphate-binding protein (PBP) and investigated its phosphate removal in water phase. When the recombinant bacteria were cultured for 6 h to treat phosphate, the removal efficiencies were 90, 49, and 41% for the treatment of 1.0, 1.5, and 2.0 mg/L phosphate, respectively, indicating good specific phosphate removal of our developed system. Also, cell densities of 2.5 and 5.0 Optical density resulted in high phosphate removal efficiencies and ~80% of 2.0 mg/L phosphate was efficiently removed. A novel biotechnology developed in this study could be effectively employed for resolving eutrophication problem in water body.

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