- Volume 24 Issue 5
Phosphate Removal Using Recombinant Bacteria with Cytoplasmic Phosphate Binding Protein
Phosphate Binding Protein이 세포질에 발현된 재조합 박테리아를 이용한 인 제거
- Choi, Suk Soon (Department of Biological and Environmental Engineering, Semyung University) ;
- Ha, Jeong Hyub (School of Environmental Science and Technology, Pohang University of Science and Technology) ;
- Cha, Hyung Joon (Department of Chemical Engineering, Pohang University of Science and Technology)
- Published : 2013.10.31
In the present work, we constructed a recombinant Escherichia coli with cytoplasmic-expressed phosphate-binding protein (PBP) and investigated its phosphate removal in water phase. When the recombinant bacteria were cultured for 6 h to treat phosphate, the removal efficiencies were 90, 49, and 41% for the treatment of 1.0, 1.5, and 2.0 mg/L phosphate, respectively, indicating good specific phosphate removal of our developed system. Also, cell densities of 2.5 and 5.0 Optical density resulted in high phosphate removal efficiencies and ~80% of 2.0 mg/L phosphate was efficiently removed. A novel biotechnology developed in this study could be effectively employed for resolving eutrophication problem in water body.
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