Antioxidant Activity of Dietary Fibers from Tubers and Stalks of Sweet Potato and Their Anti-cancer Effect in Human Colon Cancer

고구마 괴근과 잎자루 식이섬유의 항산화 효과 및 인간 대장암세포에 대한 항암작용

  • Jeong, Haeng Soon (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Joo, Hyun (Department of Physiology, College of Medicine, Inje University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
  • 정행순 (신라대학교 의생명과학대 생명공학과) ;
  • 주현 (인제대학교 의과대학 생리학교실) ;
  • 이재화 (신라대학교 의생명과학대 생명공학과)
  • Published : 2013.10.31

Abstract

Contents of the total polyphenols and flavonoids in the dietary fiber from tubers and stalks of domestic sweet potatoes were investigated. In addition, their antioxidant activity as well as the potent anti-cancer effects through the growth inhibition in human colon cancer cells (HT-29) in vitro were tested. The total flavonoids as naringin equivalents in dietary fiber from tubers and stalks of sweet potatoes were $0.5{\pm}0.001$ naringin/g extract and $2.0{\pm}0.008$ mg naringin/g extract dry basis, respectively. The amounts of the total polyphenols as gallic acid equivalents were $2.8{\pm}0.01$ mg gallic acid/g dry basis and $6.3{\pm}0.03$ mg gallic acid/g dry basis, respectively. 1,2-Diphenyi-1-picrylhydrazyl (DPPH) radical-scavenging activity of the dietary fiber from stalks was 2.4 times higher than that of the dietary fiber from tubers. Interestingly, a strong growth inhibition on HT-29 cells was observed in both dietary fibers originated from stalks and tubers of sweet potato in a dose-dependent manner. In addition, we found that the dietary fiber from tubers and stalks of sweet potato increased the gene expression of tumor suppressor p53. The great potential value in the prevention of various diseases including cancer the potential value could be confirmed through effects of the dietary fiber from tubers and stalks of sweet potato on antioxidant activity and anticancer in human colon cancer.

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