The Preparation and Release Property of Alginate Microspheres Coated Gelatin-cinnamic Acid

젤라틴-신남산 접합체가 코팅된 알긴산나트륨 마이크로스피어의 제조 및 방출 특성

  • Lee, Ju Hyup (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Jin Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Kim, Jin-Chul (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 이주협 (강원대학교 의생명과학대학 생명공학연구소) ;
  • 마진열 (한국한의학연구원 신한방제제연구센터) ;
  • 김진철 (강원대학교 의생명과학대학 생명공학연구소)
  • Published : 2013.10.31


This study is about photosensitive microspheres prepared by coating alginate microspheres with gelatin-cinnamic acid conjugate. Firstly, alginate microspheres was prepared in water-in-oil (W/O) emulsion and then they were coated with gelatin- cinnamic acid conjugate. Herein, gelatin-cinnamic acid conjugate is obtained by the amidation between an amine group of gelatin and a carboxy group of cinnamic acid. Cinnamic acid is widely used as a photo-responsive material easy to dimerize and dedimeriz under UV irradiation at ${\lambda}$ = 254 nm and ${\lambda}$ = 365 nm, respectively. As shown in SEM-EDS, alginate was successfully coated with gelatin-ciannmic acid. By determining the absorbance of coated microspheres at 270nm, the amount of cinnamic acid per microspheres was 0.13/1. The SEM photos showed the size of coated microspheres is around $10{\mu}m$. And the degrees of dimerization and dedimerization were calculated to be 49% and 23% respectively. Then the release of FITC-dextran from the coated micrspheres was studied and release the degree was 42%. As a result, the coated microspheres have potential to be used as a photo-responsive drug carrier to delivery drugs.


  1. J. M. Russell, S. H. Allan, A. P. Pauli, S. B. Lisa, and R. G. Wayne, Calcium-alginate beads for the oral delivery of transforming growth factor-${\beta}1$ (TGF-${\beta}1$) : stabilization of TGF-${\beta}1$ by the addition of polyacrylic acid within acid-treated beads, Journal of Controlled Release, 30, 3 (1994).
  2. N. M. Velings and M. M. Mestdagh, Physico-chemical properties of alginate gel beads, Polymer Gels and Networks, 3, 311 (1995).
  3. S. M. Jay and W. M. Saltman, Controlled delivery of VEGF via modulation of alginate microparticle ionic crosslinking, Journal of Controlled Release, 134, 26 (2009).
  4. M. G. Neumann, C. C. Schmitt, and E. T, Lamazaki, A fluorescence study of the interactions between sodium alginate and surfactants, Carbohydrate Research, 338, 1109 (2003).
  5. M. George and T. E. Abraham, Polyionic hydrocolloids for the intestinal delivery of protein drugs: Alginate and chitosan - a review, Journal of Controlled Release, 114, 1 (2006).
  6. M. K. Kang, J. Dai, and J.-C. Kim, Ethylcellulosemicroparticles containing chitosan and gelatin: pH-dependent release caused by complex coacervation, Journal of Industrial and Engineering Chemistary, 18, 355 (2012).
  7. A. Bigia, G. Cojazzib, S. Panzavoltaa, K. Rubinia, and N. Roveria, Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking, Biomaterials, 22, 763 (2001).
  8. K. Ulubayrama, A. N. Cakarb, P. Korkuguzb, C. Ertanc, N. Hagirci, and N. Hagirci, EGF containing gelatin-based wound dressings, Biomaterials, 22, 1345 (2001).
  9. U. G. Spizzirri, F. Iemma, F. Puoci, G. Cirillo, M. Curcio, O. I. Parigi, and N. Picci, Synthesis of Antioxidant Polymers by Grafting of Gallic Acid and Catechin on Gelatin, Biomacromolecules, 10, 1923 (2009).
  10. H. C. Liang, W. H. Chang, K. J. Lin, and H. W. Sung, Genipincrosslinked gelatin microspheres as a drug carrier for intramuscular administration: In vitro and in vivo studies, Journal of Biomedical Materials Research Part A, 65, 271 (2003).
  11. M. Gudmundsson and H. Hafsteinsson, Gelatin from cod skins as affected by chemical treatments, Journal of Food Science, 62, 37 (1997).
  12. H. L. Bruno, Investigation of viscosity and gelation properties of different mammalian and fish gelatins, Food Hydrocolloids, 5, 353 (1991).
  13. Y. Qiu and K. Park, Environment-sensitive hydrogels for drug delivery, Advanced Drug Delivery Reviews, 64, 49 (2012).
  14. E. Y. Mok, H. J. Cha, and J. C. Kim, Preparation and Characterization of Complex Composed of ${\beta}$-Cyclodextrin Polymer/Cinnamic Acid, Appl. Chem. Eng, 23, 462 (2012).
  15. J. H. Ko, H. Y. Yin, J. A, and D. J. Chung, Characterization of cross-linked gelatin nanofibers through electrospinning, Macromolecular Research, 18, 137 (2010).
  16. A. J. Ribeiro, R. J. Neufeld, P. Amaud, and J. C. Chaumeil, Microencapsulation of lipophilic drugs in chitosan-coated alginate microspheres, International Journal of Pharmaceutics, 187, 115 (1999).
  17. E. B. Denkbas and M. Odabasi, Chitosan microspheres and sponges: Preparation and characterization, Journal of Applied Polymer Science, 76, 1637 (2000).<1637::AID-APP4>3.0.CO;2-Q
  18. X. Z. Shu and K. J. Zhu, A novel approach to prepare tripolyphosphate/ chitosan complex beads for controlled release drug delivery, International Journal of Pharmaceutics, 201, 51 (2000).