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The Preparation and Release Property of Alginate Microspheres Coated Gelatin-cinnamic Acid

젤라틴-신남산 접합체가 코팅된 알긴산나트륨 마이크로스피어의 제조 및 방출 특성

  • Lee, Ju Hyup (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Jin Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) ;
  • Kim, Jin-Chul (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 이주협 (강원대학교 의생명과학대학 생명공학연구소) ;
  • 마진열 (한국한의학연구원 신한방제제연구센터) ;
  • 김진철 (강원대학교 의생명과학대학 생명공학연구소)
  • Published : 2013.10.31

Abstract

This study is about photosensitive microspheres prepared by coating alginate microspheres with gelatin-cinnamic acid conjugate. Firstly, alginate microspheres was prepared in water-in-oil (W/O) emulsion and then they were coated with gelatin- cinnamic acid conjugate. Herein, gelatin-cinnamic acid conjugate is obtained by the amidation between an amine group of gelatin and a carboxy group of cinnamic acid. Cinnamic acid is widely used as a photo-responsive material easy to dimerize and dedimeriz under UV irradiation at ${\lambda}$ = 254 nm and ${\lambda}$ = 365 nm, respectively. As shown in SEM-EDS, alginate was successfully coated with gelatin-ciannmic acid. By determining the absorbance of coated microspheres at 270nm, the amount of cinnamic acid per microspheres was 0.13/1. The SEM photos showed the size of coated microspheres is around $10{\mu}m$. And the degrees of dimerization and dedimerization were calculated to be 49% and 23% respectively. Then the release of FITC-dextran from the coated micrspheres was studied and release the degree was 42%. As a result, the coated microspheres have potential to be used as a photo-responsive drug carrier to delivery drugs.

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