DOI QR코드

DOI QR Code

Sasa borealis extract exerts an antidiabetic effect via activation of the AMP-activated protein kinase

  • Nam, Jung Soo ;
  • Chung, Hee Jin ;
  • Jang, Min Kyung ;
  • Jung, In Ah ;
  • Park, Seong Ha ;
  • Cho, Su In ;
  • Jung, Myeong Ho
  • Received : 2012.07.16
  • Accepted : 2012.11.08
  • Published : 2013.02.01

Abstract

Leaf of Sasa borealis, a species of bamboo, has been reported to exhibit anti-hyperglycemic effect. However, its antidiabetic mechanism is not fully understood. In this study, we examined whether an extract of S. borealis activates AMP-activated protein kinase (AMPK) and exerts anti-hyperglycemic effects. Treatment with the S. borealis extract increased insulin signaling and phosphorylation of AMPK and stimulated the expression of its downstream targets, including $PPAR{\alpha}$, ACO, and CPT-1 in C2C12 cells and $PPAR{\alpha}$ in HepG2 cells. However, inhibition of AMPK activation attenuated insulin signaling and prevented the stimulation of AMPK target genes. The S. borealis extract increased glucose uptake in C2C12 cells and suppressed expression of the gluconeogenic gene, PEPCK in HepG2 cells. The extract significantly reduced blood glucose and triglyceride levels in STZ-induced diabetic mice. The extract enhanced AMPK phosphorylation and increased Glut-4 expression in the skeletal muscle of the mice. These findings demonstrated that the S. borealis extract exerts its anti-hyperglycemic effect through activation of AMPK and enhancement of insulin signaling.

Keywords

Antidiabetic effect;AMP-activated protein kinase;Sasa borealis;insulin signaling

References

  1. Choi YJ, Choi JS, Shin SY, Bae JY, Kang SW, Kang IJ, Kang YH. Blockade of chronic high glucose-induced endothelial apoptosis by Sasa borealis bamboo extract. Exp Biol Med 2008;233:580-91. https://doi.org/10.3181/0707-RM-205
  2. Hwang JY, Han JS. Inhibitory effects of Sasa borealis leaves extracts on carbohydrate digestive enzymes and postprandial hyperglycemia. J Korean Soc Food Sci Nutr 2007;36:989-94. https://doi.org/10.3746/jkfn.2007.36.8.989
  3. Park EJ, Jhon DY. The antioxidant, angiotensin converting enzyme inhibition activity, and phenolic compounds of bamboo shoot extracts. Lebenson Wiss Technol 2010;43:655-9. https://doi.org/10.1016/j.lwt.2009.11.005
  4. Zhang Y, Yao X, Bao B, Zhang Y. Anti-fatigue activity of a triterpenoid-rich extract from Chinese bamboo shavings (Caulis bamfusae in taeniam). Phytother Res 2006;20:872-6. https://doi.org/10.1002/ptr.1965
  5. Choi DB, Cho KA, Na MS, Choi HS, Kim YO, Lim DH, Cho SJ, Cho H. Effect of bamboo oil on antioxidative activity and nitrite scavenging activity. J Ind Eng Chem 2008;14:765-70. https://doi.org/10.1016/j.jiec.2008.06.005
  6. Yang JH, Lim HS, Heo YR. Sasa borealis leaves extract improves insulin resistance by modulating inflammatory cytokine secretion in high fat diet-induced obese C57/BL6J mice. Nutr Res Pract 2010;4:99-105. https://doi.org/10.4162/nrp.2010.4.2.99
  7. Carling D. The AMP-activated protein kinase cascade--a unifying system for energy control. Trends Biochem Sci 2004;29:18-24. https://doi.org/10.1016/j.tibs.2003.11.005
  8. Hardie DG. Role of AMP-activated protein kinase in the metabolic syndrome and in heart disease. FEBS Lett 2008;582: 81-9. https://doi.org/10.1016/j.febslet.2007.11.018
  9. Lee MJ, Park WH, Song YS, Lee YW, Song YO, Moon GS. Effect of bamboo culm extract on oxidative stress and genetic expression: bamboo culm extract ameliorates cell adhesion molecule expression and NF${\kappa}B$ activity through the suppression of the oxidative stress. Clin Nutr 2008;27:755-63. https://doi.org/10.1016/j.clnu.2008.06.002
  10. Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, Cantley LC. The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress. Proc Natl Acad Sci U S A 2004; 101:3329-35. https://doi.org/10.1073/pnas.0308061100
  11. Hurley RL, Anderson KA, Franzone JM, Kemp BE, Means AR, Witters LA. The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem 2005;280:29060-6. https://doi.org/10.1074/jbc.M503824200
  12. Cartee GD, Wojtaszewski JF. Role of Akt substrate of 160 kDa in insulin-stimulated and contraction-stimulated glucose transport. Appl Physiol Nutr Metab 2007;32:557-66. https://doi.org/10.1139/H07-026
  13. Watson RR. Bamboo extract in the prevention of diabetes and breast cancer. In: Panee J, editor. Complementary and Alternative Therapies and the Aging Population: An Evidence-Based Approach. San Diego: Elsevier; 2009. p.159-77.
  14. Jung HJ, Nam JH, Choi J, Lee KT, Park HJ. $19\alpha$-hydroxyursane- type triterpenoids: antinociceptive anti-inflammatory principles of the roots of Rosa rugosa. Biol Pharm Bull 2005;28:101-4. https://doi.org/10.1248/bpb.28.101
  15. Park HS, Lim JH, Kim HJ, Choi HJ, Lee IS. Antioxidant flavone glycosides from the leaves of Sasa borealis. Arch Pharm Res 2007;30:161-6. https://doi.org/10.1007/BF02977689
  16. Mahato SB, Nandy AK, Roy G. Triterpenoids. Phytochemistry 1992;31:2199-249. https://doi.org/10.1016/0031-9422(92)83257-Y
  17. Cheng AY, Fantus IG. Oral antihyperglycemic therapy for type 2 diabetes mellitus. CMAJ 2005;172:213-26. https://doi.org/10.1503/cmaj.1031414
  18. Colberg SR, Zarrabi L, Bennington L, Nakave A, Thomas Somma C, Swain DP, Sechrist SR. Postprandial walking is better for lowering the glycemic effect of dinner than pre-dinner exercise in type 2 diabetic individuals. J Am Med Dir Assoc 2009;10:394-7. https://doi.org/10.1016/j.jamda.2009.03.015
  19. Yin J, Zhang H, Ye J. Traditional Chinese medicine in treatment of metabolic syndrome. Endocr Metab Immune Disord Drug Targets 2008;8:99-111. https://doi.org/10.2174/187153008784534330
  20. Bailey CJ, Day C. Traditional plant medicines as treatments for diabetes. Diabetes Care 1989;12:553-64. https://doi.org/10.2337/diacare.12.8.553
  21. Lu B, Wu X, Shi J, Dong Y, Zhang Y. Toxicology and safety of antioxidant of bamboo leaves. Part 2: developmental toxicity test in rats with antioxidant of bamboo leaves. Food Chem Toxicol 2006;44:1739-43. https://doi.org/10.1016/j.fct.2006.05.012
  22. Kim EY, Jung EY, Lim HS, Heo YR. The effects of the Sasa borealis leaves extract on plasma adiponectin, resistin, C-reactive protein and homocysteine levels in high fat diet-induced obese C57/BL6J mice. Korean J Nutr 2007;40:303-11.
  23. Ko BS, Jun DW, Jang JS, Kim JH, Park S. Effect of Sasa borealis and white lotus roots and leaves on insulin action and secretion in vitro. Korean J Food Sci Technol 2006;38:114-20.

Cited by

  1. Leaf Extract Retard the Digestion of Starch and Its Working Mechanisms vol.62, pp.31, 2014, https://doi.org/10.1021/jf501931m
  2. Sasa borealis leaf extract-mediated green synthesis of silver–silver chloride nanoparticles and their antibacterial and anticancer activities vol.41, pp.3, 2017, https://doi.org/10.1039/C6NJ03454C
  3. leaf extract-mediated gold nanoparticles pp.2169-141X, 2017, https://doi.org/10.1080/21691401.2017.1293675
  4. Anti-Hypertensive Action of Fenofibrate via UCP2 Upregulation Mediated by PPAR Activation in Baroreflex Afferent Pathway pp.1995-8218, 2018, https://doi.org/10.1007/s12264-018-0271-1

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)